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Method for preparing fuel of ethanol from immobilized mixed strain fermented cellulosic hydrolysate

A technology of cellulose hydrolyzate and mixed bacterial species, applied in biochemical equipment and methods, microorganism-based methods, fixed on/in organic carriers, etc., can solve problems such as shortening the fermentation cycle, and shorten the fermentation cycle. , Eliminate the effect of substrate inhibition and enhanced antibacterial ability

Inactive Publication Date: 2009-10-14
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to make full use of the hexose and xylose in the cellulose hydrolyzate, the co-fermentation of immobilized mixed strains is used to produce fuel ethanol. In order to solve the problems existing in the fermentation of immobilized mixed strains, a new fermentation strain using xylose Co-immobilization with Saccharomyces cerevisiae, using fed-batch fermentation to ferment to increase ethanol concentration, shorten the fermentation cycle, and improve the fermentation capacity of the strain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The suspensions of Pachysomyces tannophilus and Saccharomyces cerevisiae that were domesticated and cultivated were added to 3% sodium alginate solution at a ratio of 1:1, and injected with 2% CaCl 2 In the aqueous solution, solidify for 4 hours, wash the sodium alginate gel balls (diameter 2-3mm) with sterile water, transfer to 1% aluminum sulfate solution, and cure at 0-4°C for 12-24 hours to obtain gel balls with immobilized mixed strains . The immobilized gel balls were placed in the proliferation medium, and cultured for 48 hours at 30°C and 100 r / min. The concentration is 143.41g / L, the pH value is adjusted to 5.6, the volume of the fermentation broth and the volume of the gel balls are 3:2, fermented on a constant temperature shaker at 30°C and 100r / min, fermented for 12h, and the alcohol concentration is determined to be 11.42g / L, the alcohol yield is 53.6%.

Embodiment 2

[0018] Others are the same as in Example 1, the immobilized mixed bacterial strains that have proliferated are moved into the cellulose hydrolyzate fermentation medium, the initial sugar concentration is 88.428g / L, the pH value is adjusted to 5.7, and the volume of the fermentation broth and the accumulation volume of the gel balls are 3:2, fermented on a constant temperature shaker at 30°C and 100r / min, supplemented with fresh hydrolyzate after an interval of 12 hours, and fermented for 24 hours, the alcohol concentration was determined to be 15.09g / L, and the alcohol yield was 78.5%.

Embodiment 3

[0020] Others are the same as in Example 1, the immobilized mixed bacterial strains that have proliferated are moved into the cellulose hydrolyzate fermentation medium, the initial sugar concentration is 118.42g / L, the pH value is adjusted to 6.0, and the bulk volume of the fermented liquid volume and the gel ball is 3:2, fermented on a constant temperature shaker at 32°C and 110r / min, supplemented with fresh hydrolyzate after an interval of 12 hours, fermented for 24 hours, the alcohol concentration was 19.84g / L, and the alcohol yield was 83.13%.

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Abstract

The invention relates to a method for preparing fuel of ethanol from immobilized mixed strain fermented cellulosic hydrolysate, which is characterized by utilizing mixed strains of P. tannophilus and S. cerevisilae to graft into diluted acid hydrolysate and fermenting the mixed solution by a supplemented material batch fermentation mode. The method is realized by the following steps: (1) domestication of the P. tannophilus and the S. cerevisilae; (2) immobilization of the mixed strains by calcium alginate; (3) multiplication culture of the immobilized mixed strains; and (4) supplemented material batch fermentation of the immobilized mixed strains. The immobilized mixed strains can be fermented while using xylems and glucose in the hydrolysate for fermentation, thereby the fermentation period is shortened, the utilization ratio of the xylose and the glucose is improved and the ethanol concentration is increased; the immobilized mixed strains can be repeatedly used and have strong anti-hybrid bacteria capability; the supplemented material batch fermentation mode is applied to hydrolysate with cellulose raw material for producing the fuel of ethanol, thereby reducing inhibiting factors in the hydrolysate; and the method is beneficial to thoroughly eliminating the inhibition on substrates, diluting poisonous metabolite, reducing contaminated bacterial, controlling the fermentation time and preventing the generation of side products.

Description

technical field [0001] The invention relates to a method for preparing fuel ethanol with immobilized mixed strains, in particular, it is prepared by fermenting lignocellulose hydrolyzate with immobilized mixed strains of Pachysolen tannophilus and Saccharomyces cerevisiae Process for the production of fuel ethanol. Background technique [0002] Ethanol is an important industrial raw material, which is widely used in the fields of chemical industry, food, military industry, daily chemical industry, medicine and health, and can also be used as the basic raw material and fuel of the energy industry. Today's world is facing the challenges of environment and development, and the call for using unleaded gasoline is growing louder. The ethanol is further dehydrated and then added an appropriate amount of gasoline to form deformed fuel ethanol. Abroad, ethanol is regarded as the best fuel to replace and save gasoline. As a kind of bioenergy, ethanol is expected to replace the dwind...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P39/00C12N11/04C12R1/865C12R1/645
CPCY02E50/16Y02E50/10
Inventor 魏刚李艳华魏云鹏张雯房彩琴熊蓉春
Owner BEIJING UNIV OF CHEM TECH
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