Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof

A technology of Paecilomyces lilacinus and Verticillium chlamydoides, which is applied in the field of nematode-killing compositions and preparations using nematode parasites, which can solve the problems of pesticide varieties losing effectiveness, long development cycle of new pesticides, and lack of support, etc. problems, to overcome the long cycle of new drug research and development, promote growth, and have good effect on killing nematodes

Active Publication Date: 2012-09-12
GUANGDONG ZHENGE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to long-term, single use or abuse of pesticides in agricultural production, pests have developed resistance to pesticides, and even developed cross-resistance and multi-resistance, ma

Method used

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  • Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof
  • Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof
  • Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof

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Effect test

preparation example Construction

[0039] A preparation method of a nematicidal composition containing Verticillium chlamydia and Paecilomyces lilacinus combined, comprising the following steps:

[0040] (1) Evenly disperse the parent powder of Verticillium chlamydia and Paecilomyces lilacinus in a homogenizer equipped with solvents and additives to obtain a mixture of Verticillium chlamydia and Paecilomyces lilacinus with uniform viscosity bacterial liquid;

[0041] (2) Transfer the prepared mixed bacterial solution to a high-level container;

[0042] (3) Put the carrier into the mixer;

[0043] (4) Start the agitator, open the drain valve of the high-level container, and add the mixed bacteria solution until the bacteria solution is added;

[0044] (5) Check the effect of making granules by the adsorption method, and check the technical indicators of the products.

Embodiment 1

[0045] The research of embodiment 1 synergism

[0046] In order to overcome and delay the pesticide resistance of pests and prolong the service life of old pesticide varieties, we used Verticillium chlamydia and Paecilomyces lilacinus to compound pesticides to study the synergistic effect on root-knot nematode incognita.

[0047] Pick tomato roots artificially inoculated with root-knot nematode incognita, crush them with a tissue masher, pass through 200-mesh, 325-mesh, and 500-mesh nematode standard sieves in turn, wash the eggs on the 500-mesh sieve with sterile water, and prepare eggs Suspension (100 eggs / mL). Another part of the eggs were hatched in sterile water, and the live larvae (2nd instar larvae) hatched on the second day were taken for testing. The fresh Paecilomyces lilacinus fermented liquid was prepared into a certain concentration of mother liquid, and then mixed with Verticillium chlamydoides according to (6:1, 4:1, 2:1, 1:1 and 1:2), the test Suspension of ...

Embodiment 2

[0048] The preparation of embodiment 2 compositions

[0049] The present invention provides a case of a nematicide granule containing a combination of Verticillium chlamydia and Paecilomyces lilacinus and its preparation method:

[0050] Each component and content in the granule can be according to 1) 50 grams of living mother powder containing 2.5 billion / gram Verticillium chlamydia, 50 grams of mother powder containing 20 billion / gram Paecilomyces lilacinus, 50 grams of stabilizer soybean oil gram, solvent mineral oil 100 grams, carrier kaolin 750 grams. 2) Containing 2.5 billion / g Verticillium chlamydia live mother powder 50 grams, containing 20 billion / g Paecilomyces lilacinus mother powder 50 grams, solvent methyl oleate 50 grams, stabilizer castor oil 50 grams, carrier About 800 grams of kaolin.

[0051] The nematicide granules of Verticillium chlamydia and Paecilomyces lilacinus are prepared through the following steps: (1) Mixing the parent powder of Verticillium chl...

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Abstract

The invention relates to a nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and a preparation method thereof. The nematicidal composition consists of the verticillium chlamydosporium and the Paecilomyces lilacinus, and also comprises a solvent, an aid and a stabilizer. The nematicidal composition comprises the following components in percentage by weight: 0.1 to 10 percent of verticillium chlamydosporium, 0.1 to 20 percent of Paecilomyces lilacinus, 1 to 15 percent of stabilizer, 5 to 15 percent of solvent, 1 to 10 percent of aid and 60 to 90 percent of carrier. The verticillium chlamydosporium and the Paecilomyces lilacinus are combined to achieve a synergistic effect, and a nematicidal effect is better; the verticillium chlamydosporium and the Paecilomyces lilacinus are bacterial parasites for nematode, a biocontrol technique is used, and is environment-friendly and safe, and problems that the nematode has resistance to pesticides, a development cycle for a new pesticide is long and the like are solved; and the verticillium chlamydosporium and the Paecilomyces lilacinus can promote the growth of plants to achieve effects of increasing production and value.

Description

technical field [0001] The present invention relates to a nematode-killing composition, in particular to a nematode-killing composition with a nematode parasite and a preparation method thereof. Background technique [0002] Root-knot nematodes mainly damage the roots of various vegetables, showing more lateral roots and fibrous roots than normal, and forming spherical or conical white nodules of various sizes on the fibrous roots of young roots, some of which are bead-shaped. The aboveground part of the damaged plant grows short and slow, with abnormal leaf color, few fruits, low yield, and even premature death of the plant. The nematodes currently known to damage vegetables mainly include root-knot nematode high pedicle, root-knot nematode peanut, root-knot nematode northern and root-knot nematode southern. Nematodes have a wide range of hosts and often damage more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, carrots, lettuce, and cabba...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/08A01P5/00
CPCY02A40/10
Inventor 赵勇王泊理高英超张天成
Owner GUANGDONG ZHENGE BIOLOGICAL TECH CO LTD
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