Preparation method of paecilomyces lilacinus spore powder

A technology of Paecilomyces lilacinus and spore powder, which is applied in the field of production of Paecilomyces lilacinus spore powder, can solve the problems of low recovery rate and low number of live spores, achieve low water content, high spore recovery rate, and improve Effects on Yield and Product Quality

Active Publication Date: 2013-02-13
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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AI Technical Summary

Problems solved by technology

[0005] The quality of Paecilomyces lilacinus biopesticide inoculum is mainly based on the number of spores produced. The existing preparation methods of Paecilomyces lilacinus spore powders all have low recovery rate and low number of viable spores to varying degrees. Other defects need to be improved

Method used

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  • Preparation method of paecilomyces lilacinus spore powder
  • Preparation method of paecilomyces lilacinus spore powder
  • Preparation method of paecilomyces lilacinus spore powder

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Embodiment 1

[0029] The preparation of embodiment 1 Paecilomyces lilacinus (P.lilacinus) fermented liquid

[0030] 1. After activating the Paecilomyces lilacinus species, carry out shake flask seed culture;

[0031] The composition of shake flask seed culture medium (by mass percentage):

[0032]

[0033] Liquid fermentation conditions: put 200ml of seed medium into a 500ml triangular flask, inoculate Paecilomyces lilacinus spore suspension after autoclaving, the inoculation amount is 1.5-3%, place at 24-28°C, shaker 150-200r / min, cultivated for 18-36h.

[0034] 2. Secondary liquid expansion culture:

[0035] The medium composition (by mass percentage) of secondary liquid expanded culture:

[0036]

[0037] In-situ sterilization of the above-mentioned secondary liquid expansion medium fermenter;

[0038] Liquid fermentation conditions: fermentation temperature 25-28°C, tank pressure 0.6-0.8bar, oxygen flow rate 100-300L / h, rotation speed 150-250rpm, initial pH value 5.5-6.0.

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Abstract

The invention discloses a preparation method of paecilomyces lilacinus spore powder. The method comprises the following steps of: (1) preparing a paecilomyces lilacinus liquid fermentation broth; (2) recovering conidia from the paecilomyces lilacinus liquid fermentation broth; and (3) drying the recovered paecilomyces lilacinus conidia to obtain the paecilomyces lilacinus spore powder. According to the invention, the technological parameters for preparing paecilomyces lilacinus spore powder are screened and optimized to obtain optimal technological parameters for preparation, thus the yield and product quality of paecilomyces lilacinus spores are effectively improved. The preparation method of paecilomyces lilacinus spore powder, disclosed by the invention, has the advantages of high recovery rate, high spore content, high live spore rate, low water content and the like, and conforms to the requirements of large-scale industrial production.

Description

technical field [0001] The invention relates to a preparation method of fungal spore powder, in particular to a preparation method of Paecilomyces. lilacinus spore powder, and belongs to the production field of Paecilomyces. lilacinus spore powder. Background technique [0002] Paecilomyces lilacinus (P.lilacinus) is an endoparasitic fungus and is an important natural enemy of some plant parasitic nematodes. It can parasitize eggs and infect larvae and females. Cyst nematodes, stem nematodes and other plant nematodes. [0003] Plant parasitic nematodes are the second largest group of crop pathogens after fungi. They are widely distributed and can parasitize and harm a variety of crops. Root-knot nematode is a kind of obligate plant parasitic pathogen. Most of the nematicides used in production are highly toxic chemical pesticides with high toxicity, which not only affects human health, but also poses a potential threat to the environment. Compared with the traditional use ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/79
Inventor 汪来发王曦茁郭志斌林彩丽田国忠马建伟
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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