Method for extracting liquiritigenin from liquorice

A technology of glycyrrhizin and licorice, applied in the direction of organic chemistry, can solve the problems of poor efficiency and low content

Active Publication Date: 2012-03-28
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is already one patent for the extraction of glycyrrhizin. Chinese patent CN102112142A is used to increase the content of glycyrrhizin in licorice or licorice extract. It is first extracted with water or organic solvent, then acid hydrolyzed, and does not use resin column. poor, low content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The method for extracting glycyrrhizin from licorice, comprises the steps:

[0020] (1) Grind licorice into 60-mesh powder, extract with hydrochloric acid solution with a concentration of 1mol / L for 120 minutes at 80°C and a mass ratio of solid to liquid of 1:5, filter with four layers of gauze, and wait for the filtrate to cool to room temperature , adding a 1mol / L sodium hydroxide solution to adjust to neutrality to obtain a crude extract of glycyrrhizin;

[0021] (2) Put the crude extract of liquiritigenin on the mixed resin column, the mixed resin is composed of polyamide resin 30-60 mesh and macroporous resin HPD-100 with a mass ratio of 4:6, and use 5 times the column volume of deionized water , the volume percent concentration of 5 times the column volume is 20% ethanol aqueous solution, and the volume percent concentration of 4 times the column volume is 70% ethanol aqueous solution for elution, and the collected volume percent concentration is 70% ethanol aqueo...

Embodiment 2

[0023] The method for extracting glycyrrhizin from licorice, comprises the steps:

[0024] (1) Crush licorice into 60-mesh powder, extract with hydrochloric acid solution with a concentration of 1mol / L for 120 minutes at 80°C and a mass ratio of solid to liquid of 1:7, filter with four layers of gauze, and wait for the filtrate to cool to room temperature , adding a 1mol / L sodium hydroxide solution to adjust to neutrality to obtain a crude extract of glycyrrhizin;

[0025] (2) Put the crude glycyrrhizin extract on the mixed resin column, the mixed resin is composed of polyamide resin 60-100 mesh and macroporous resin D101B with a mass ratio of 4:6, and use 6 times the column volume of deionized water, 5 The volume percent concentration of 3 times column volume is 30% ethanol aqueous solution, and the volume percent concentration of 4 times column volume is 70% ethanol aqueous solution for elution, and the collected volume percent concentration is 70% ethanol aqueous solution e...

Embodiment 3

[0027] The method for extracting glycyrrhizin from licorice, comprises the steps:

[0028] (1) Grind licorice into 60 mesh powder, extract with hydrochloric acid solution with a concentration of 1mol / L for 120 minutes at 80°C and a mass ratio of solid to liquid of 1:8, filter with four layers of gauze, and wait for the filtrate to cool to room temperature , adding a 1mol / L sodium hydroxide solution to adjust to neutrality to obtain a crude extract of glycyrrhizin;

[0029] (2) Put the crude extract of liquiritigenin on the mixed resin column, the mixed resin is composed of polyamide resin 60-100 mesh and macroporous resin HP-30 with a mass ratio of 4:6, and use 7 times the column volume of deionized water , the volume percentage concentration of 5 times the column volume is 40% ethanol water solution, and the volume percentage concentration of 4 times the column volume is 70% ethanol water solution for elution, and the collection volume percentage concentration is 70% ethanol ...

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PUM

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Abstract

The invention discloses a method for extracting liquiritigenin from liquorice. The method comprises the following steps of: (1) crushing the liquorice, extracting the powder by using 1 mol/L hydrochloric acid solution, filtering, regulating the filtrate to be neutral by using a 1 mol/L sodium hydroxide solution, and obtaining a crude liquiritigenin extract; and (2) injecting the crude liquiritigenin extract into a mixed resin column, and performing elution, separation, purification and re-crystallization. The liquiritigenin extracted by the method is a natural extract, and is high in purity, low in cost, high in production efficiency and stable and controllable in quality.

Description

technical field [0001] The invention belongs to a method for extracting and purifying liquiritigenin, in particular to a method for extracting liquiritigenin from licorice. Background technique [0002] Licorice is the dried roots and rhizomes of Glycyrrhiza uralensis Fisch., G. inflata Bata1., and G. glabra L. of the leguminous plants Glycyrrhiza uralensis Fisch. Clearing heat and detoxifying, eliminating phlegm and relieving cough, relieving spasm and relieving pain, and harmonizing the effects of various medicines. The flavonoids in licorice have a variety of biological activities. In addition to anti-inflammatory, antibacterial, and anti-allergic effects, they also have obvious effects on anti-oxidation, anti-cancer, anti-cancer, and anti-tumor. Modern pharmacological studies have shown that liquiritin, isoliquiritin, liquiritigenin and isoliquiritigenin are the main active ingredients of licorice flavonoids. [0003] Liquiritigenin is an isomer of isoliquiritigenin, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32C07D311/40
Inventor 吴巍
Owner 天津市尖峰天然产物研究开发有限公司
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