106 results about "Hair Papilla" patented technology

The invention relates to a preparation method of tissue engineering skin containing hair follicles. In the invention, tissue engineering hair follicles are evenly inoculated in a tissue engineering dermal layer formed by compounding a bracket material and cells, then the tissue engineering dermal layer is covered by epidermal cells, and the tissue engineering skin containing the hair follicles is formed after culture; the invention overcomes the defects of little number of the hair follicles in the skin, no uniformity and uncontrollable quantity of the hair follicles caused by mixed injection formerly, and the prepared tissue engineering skin contains an epidermis, dermis and hair follicle type tissue structure, wherein the number of the hair follicles approaches to the natural number, the hair follicles are evenly distributed in artificial pores and develop continuously after in-vitro culture and transplantation, a multi-layer concentric circle structure is formed, an inner layer is hair papilla cells wrapt by micro-capsule membranes, and an outer layer is epithelium class cells; and the skin cultured in vitro or transplanted in vivo finally forms functional skin containing a hair follicle accessory structure, and the regeneration of the hair follicles after skin transplantation can be realized.

A method for the preparation of dermal papilla tissue comprising the step of culturing mesenchymal stem cells in a medium having a specific composition is provided. The method makes it possible to form in vitro a quantity of dermal papilla tissues having hair follicle inducting ability and, accordingly, it can be effectively used for the treatment of alopecia through cell transplantation.

The invention relates to Chinese medicine hair nourishing shampoo which is capable of prompting hair papilla compendency growth, and a preparation method of the Chinese medicine hair nourishing shampoo. The Chinese medicine hair nourishing shampoo comprises the following functional components in parts by weight: 10-50 parts of polygonum cuspidatum, 0.5-20 parts of pepper, 0.5-20 parts of dry ginger, 0.1-10 parts of semen plantaginis and 0.5-6 parts of chili. A great deal of experiment shows that the extract of the Chinese medicine composition is capable of stimulating growth activity of hair papilla cells, so that the effects of preventing alopecia and nourishing hair are achieved, and moreover the Chinese medicine extract can be prepared into hair shampoo which is convenient to use by patients.

The invention relates to a preparation method of tissue engineering skin containing appendant organs. In the invention, the preparation method comprises the following steps of: inoculating amniotic mesenchyme stem cells, amniotic mesenchyme stem cells induced to the directions of hair papillae, amniotic epithelial cells and amniotic epithelial cells induced to the directions of the epithelia of the sweat gland into a gel solution, then inoculating the amniotic epithelial cells and the amniotic epithelial cells induced by keratinocyte in a warp direction on the surface of a structure of the hair follicle and the sweat gland after culturing by a dermal layer by induced culture forming the structure of the hair follicle and the sweat gland, and obtaining the tissue engineering skin with the structure of the hair follicle and the sweat gland after culturing. The skin has elasticity, toughness, vigorous cellular metabolism, strong multiplication capacity, sufficient secretion of extracellular matrices, tight linkage of the cuticular layer, the inophragma and the cells of the dermal layer and little possibility of falling off, enhances the success rate of transplantation, can promote the healing of a wound surface, enhances the effects of restoration and replacement and has the function of regulating the immunological rejection of a receptor; by the contained structure of the hair follicle and the sweat gland, the function of the skin is more complete; the adopted amniotic tissues are postpartum wastes and have extensive source, strong cell multiplication capability and multiplepassage frequency; and the invention is beneficial to the mass production of seed cells and lowers the industrialization cost.

The present invention relates to a preparation method of artificial skin containing hair follicle. Said method includes the following steps: separating mouse palp hair follicle, adding resolvase to make digestion, centrifugalizing to obtain hair follicle epithet lial cell, taking out mouse palp hair follicle whose hair shaft is removed, using collagenase D to make digestion, centrifugalizing, using DMEM culture medium to make dermal sheath cell culture and make hair papilla cell culture; making the subcultured cell be respectively mixed with hair follicle epithelial cell according to the ratio of 5-1:1-5, and transplanting on collage / chtosan porous support, adding DMEM culture medium to make culture so as to obtain the invented product.

The invention relates to a combination for promoting eyelash growth. The combination comprises myristoyl pentapeptide-4, palmitoyl tripeptide-1 and tripeptide-1 copper. The invention further provides an application of the combination. The combination has the advantages of capabilities of activating keratin gene expression of human bodies to generate more keratin, and promoting eyelash growth. Eyelash growth solution prepared by the combination can promote eyelashes to be long and dense and is mild and non-irritating, functional components can rapidly enter hair follicle and skins and reach skin bottoms to activate dermal papilla cells.

A process for delivering hair removal fluids to hair ducts. In a preferred embodiment the fluid is a vaccine for slowing or stopping hair growth and a process for applying the vaccine or other hair growth vaccines to the skin region having unwanted hair and a method of delivering the vaccine to skin regions of unwanted hair. The preferred vaccine consists of an antigen cocktail of weakened or essentially dead elements of hair follicles for stimulating antibody responses. The preferred vaccine is prepared from patient's own hair papilla or bulge area stem cells. In other embodiments vaccines are made from extracts of papilla cells cultivated in a laboratory. The vaccines produce antibodies and these antibodies attack and destroy hair papilla and block bulge area cells that would otherwise produce or stimulate a growth of new papilla cells. Even if any new papilla cells appear, the circulating antibodies of the vaccine-activated immune system attack and destroy them also. Other hair removal fluids that can be applied with the process of this invention are also described.

The invention provides a hair restorer. The hair restorer comprises an adipose tissue-derived stromal cell conditioned medium, a fibroblast exosome, mannitol, trehalose and dextran 40, wherein adiposetissue-derived stromal cells are irradiated according to the UVB irradiation dosage of 20-100 mJ / cm<2>, the irradiated adipose tissue-derived stromal cells secrete various types of bioactive factors,hair papilla can be stimulated, hair follicle stem cells can be activated, and the function of hair restoration from inside to outside is realized. The invention further provides a hair restoration composition with a nutritional agent and the hair restorer. The hair restoration composition has a remarkable treatment effect on androgenic alopecia, alopecia seborrhoeica and alopecia areata and is easy to use and popularize.

The invention discloses an external composition for preventing alopecia and promoting hair growth and a preparation method thereof. The method comprises the following raw materials including adenosine, glycyrrhetinic acid, apple polyphenol, baicalin, alcohol and water. The method has the advantages that since hair papilla cells positioned at the central bottoms of hair follicles generate and release proliferation factors, the proliferation and differentiation of hair follicle epithelial stem cells are influenced, and therefore the hair cycle is controlled; the baicalin can maintain the derivatization ability of the hair follicles, the adenosine can improve the proliferation of the hair papilla cells, the glycyrrhetinic acid can prevent the apoptosis of the hair papilla cells, and the applepolyphenol, especially dimers thereof, can promote the differentiation and growth of keratinocytes and protein synthesis. All raw materials of the external composition for preventing alopecia and promoting hair growth are organically combined together, correspondingly the number of the hair follicles can be effectively increased, the vitality of the hair papilla cells in the hair follicles can bemaintained, and the effects of integrally promoting the hair growth and reducing the hair loss are thus achieved.

The invention relates to a vegetal anti-alopecia hair-renewal nutrient solution. The vegetal anti-alopecia hair-renewal nutrient solution is prepared from, by weight, 80-90 parts of plant extracting solutions, 0.1-0.3 part of lycium ruthenicum murr anthocyanin, 8-12 parts of ferment, 0.1-0.3 part of panaxoside, 7-11 parts of butanediol and 0.1-0.3 part of salicylic acid. The vegetal anti-alopecia hair-renewal nutrient solution can balance scalp grease secretion, enhance circulation of micro-thin blood vessels, promote blood flow, activate atrophic and dormant hair follicle cells to participate into blood-oxygen exchange with the organism, push follicle cells to vigorously divide, cultivate hair papilla once again and renew hair and meanwhile has the effects of resisting to bacteria, diminishing inflammation, removing scurf, relieving itching and enabling the hair to be black and glossy.

The invention provides a compound external preparation for preventing and treating alopecia and promoting hair growth. The compound external preparation comprises the following specific components andformula weight proportions: every 100 ml of a solvent contains 1 to 50 g of metformin, 0.1 to 10 mg of finasteride, 0.1 to 50 g of minoxidil, 0.1 to 50 mg of vitamin B1 and 0.1 to 50 mg of vitamin B6; and the solvent is water or ethanol. Compared with single minoxidil, metformin, vitamins B1+B6 or combination of two drugs (combination of oral administration of the finasteride and external application of the minoxidil, combination of the minoxidil and the vitamin B1), the compound external preparation has the advantages that the hair follicle stem cells can be activated, and more hair folliclecells enter the growth period after the rest period is ended; new hair follicles in the growing period can be longer, and hair papillae are larger in size; and the component medicines in a formula can achieve a synergistic effect, and the compound external preparation has remarkable effects in the aspects of treating the alopecia and promoting the hair growth.

The invention discloses application of a hair follicle stem cell-derived exosome in promoting hair follicle stem cell proliferation and differentiation into hair follicle cells. It is found that the exosome derived from the hair follicle stem cells can enter recipient cells, and can promote proliferation of the hair follicle stem cells and dermal papillae cells, wherein the hair follicle stem cell-derived exosome stimulated by melatonin has a more obvious effect of promoting hair follicle stem cell proliferation. It is further found that the hair follicle stem cell-derived exosome can promotemigration of hair follicle stem cells and dermal papillae cells, and the migration promoting effect of the melatonin-stimulated hair follicle stem cell-derived exosome is more obvious. The hair follicle stem cell-derived exosome can promote the expression of hair follicle development-related genes of hair follicle stem cells and dermal papillae cells so as to promote the differentiation of the hair follicle stem cells into the hair follicle cells, and the melatonin-stimulated hair follicle stem cell-derived exosome has a more obvious effect of promoting the proliferation and differentiation ofthe hair follicle stem cells.

The invention belongs to the field of cosmetics and relates to novel application of leech, i.e. application of the leech to nursing of hair or scalp of a head, a composition which is prepared by using the leech as the main raw material and is used for nursing the hair or the scalp of the head, and a preparation method of the composition. For the novel application of the leech, the effects of hirudin on activating blood, dissolving stasis, promoting blood circulation and removing obstruction in channels are utilized for nursing the hair or the scalp of the head, so that blood circulation of the head is strengthened, the blood flow is enhanced, the scalp is nourished, capillary waste of the scalp is removed, melanin is timely conveyed for hair papilla and the hair is blackened and brightened. A research process also shows that the hirudin has capabilities of promoting the metabolism of the scalp, activating hair follicle tissues and strengthening hair roots so as to achieve the effects on hair promotion, hair nourishment and alopecia prevention, fundamentally improve the physiological status of the hair, promote the hair growth and integrate three effects on alopecia prevention, alopecia treatment and hair growth into a whole; and the hirudin also has an effect of repairing damaged hair, so that the hair becomes smooth and tenacious.

Disclosed in the present specification are: a composition for preventing hair loss or promoting hair growth, containing ginseng-derived exosome-like vesicles; and a method for preparing the composition. The ginseng-derived exosome-like vesicles can have a diameter of 20-500 nm and can be isolated from ginseng root. The composition contains ginseng-derived exosome-like vesicles as active ingredients, thereby having an effect of inducing hair growth by activating dermal papilla cells (DPCs).

The invention discloses traditional Chinese medicine cream for preventing hair loss, relates to a hair loss preventer and in particular relates to a traditional Chinese medicine hair loss preventer. The traditional Chinese medicine cream is characterized by consisting of a cream shampoo part and a medicinal cream part, wherein the cream shampoo and the medicinal cream are simultaneously used, and the medicinal cream is applied to important acupuncture points such as Baihui acupoint, temple, Yuzhen and Fengchi according to actual situation. According to thermal moxibustion and multiple drug stimulation massage, blood circulation of local hair follicles is accelerated, so that hair papilla can obtain sufficient blood supply, and hair growth is promoted. Moreover, due to nourishing of traditional Chinese medicines, the cream has obvious effects of preventing and treating alopecia seborrhoeica and psychogenic alopecia syndromes, also has the effects of enhancing hair toughness and elasticity and repairing damaged hair and does not have any toxic or side effect.

A process for preparing the medicine to treating alopecia areata includes such steps as preparing the basic culture medium from human AB-type serum, low-sugar culture medium (DMEM), Hepes, sodium bicarbonate and L-glutamine proportionally, culturing the low-passage human hair papilla cells, taking the supernatant and freeze-drying to obtain powder.

The invention discloses a method for restoring the dryness or proliferative ability of hair follicle-derived cells, comprising the following steps: cutting umbilical cord with removed arteries and veins, culturing by adding a low-sugar DMEM culture solution containing bovine serum and penicillin-streptomycin, and carrying out subculture by trypsinization when mesenchymal stem cell fusion reaches 70-80%; continuously culturing by replacing a medium without exosome serum when the cells are passaged to the 3rd to 5th generation and cell fusion reaches 60-80%; extracting by a ultracentrifugation method to obtain exosomes of mesenchymal stem cells; carrying out isolated culture on dermal papillae cells by an improved two-step enzymatic method; and culturing the obtained dermal papillae cells ina DEME basal medium, which contains PDGF, KGF, VEGF and exosomes of mesenchymal stem cells. The method of the invention can effectively restore the dryness of hair follicle-derived cells, and regulates the normal physiological metabolism of hair follicle cells by secreting various regulatory factors, thus solving the problem of alopecia from the perspective of hair follicle physiology.

A method for photothermolysis that includes applying galvanic current energy to skin at an area of a hair follicle and applying pulsed optical energy to the skin at the area of the hair follicle at an energy level and duration so as to cause thermal destruction of a hair papilla.

The present invention relates to gene engineering, and is one kind of expression vector of human hair papilla cell HSPC016 gene. The vector is plasmid pET-22b(+) containing the functional sequence of HSPC016 gene, and the HSPC016 coded sequence is located between Nde I and Xho I cleavage site of pET-22b(+) vector. The present invention also relates to one kind of gene engineering bacterium transformed with the said vector and provides process of preparing human hair papilla cell HSPC016 target protein. The HSPC016 target protein is expressed through inducing colibacillus pET-22b(+) / HSPC016 / BL21 with IPTG. The present invention has the advantages of high target protein expressing amount, expression rate over 28 %, addition of 6xHis purification label for easy protein purification, high bioactivity of the target protein, etc.

The present invention provides an agent for activating proliferation of follicle dermal papilla cells comprising an acidic saccharide alkaline earth metal salt, wherein the acidic saccharide is selected from the group consisting of a phosphorylated saccharide and lactobionic acid. The acidic saccharide is preferably a phosphorylated saccharide. The alkaline earth metal salt is preferably a calcium salt or a magnesium salt. In accordance with the present invention, the proliferation of follicle dermal papilla cells is promoted. Thus, the agent for activating proliferation of follicle dermal papilla cells is expected to produce hair-restoration and hair-growth effects such as treatment of hair thinning, prevention of hair loss, promotion of hair restoration, and promotion of hair growth.

This invention provides an experimental animal which carries hair follicles which have been reconstituted with use of hair papilla cells, epidermal cells and melanocytes which are extraneous to these cells. Such an experimental animal is usable for evaluating factors which influence hair restoration and the depth of hair color.

The invention relates to hair restoring liquid containing a dermal papilla cell derived exosome and collagen and a preparation method thereof. The hair restoring liquid comprises an exosome composition and accessories; and the exosome composition comprises the dermal papilla cell derived exosome and the collagen coated in the dermal papilla cell derived exosome. Main functional components of the hair restoring liquid to which the invention relates are the dermal papilla cell derived exosome and the collagen coated in the dermal papilla cell derived exosome; the dermal papilla cell derived exosome and the collagen supplement each other and take synergetic effects; proliferation activity and migratory activity of hair matrix cells can be promoted; hair oiliness and the baldness phenomenon ofa patient suffering from serious baldness can be obviously improved; and growth of restored hair is promoted.

The present invention relates to a composition containing sophorolipid as an active ingredient for preventing hair loss or promoting hair growth. According to the present invention, sophorolipid, which is a non-toxic and biodegradable natural surfactant derived from microbes, is environmentally friendly, and sophorolipid, when treated on hair papilla cells, exhibits a growth promotion effect of the hair papilla cells, remarkably decreases the number of shed hairs, and shows a hair growth promotion effect, and thus can be favorably used as a cosmetic agent or externally-applied dermal preparation for preventing hair loss, relieving hair loss, treating hair loss, or promoting hair growth.

An artificial hair papilla for treating the hypotrichosis and alopecia is composed of the hair papilla cells and a three-layer hollow microbag consisting of sodium alginate, polylsine and sodium alginate layers for containing said hair papilla cells in it.

The invention relates to a hair growth liquid containing a dermal papilla cell-derived exosome and a preparation method thereof. The hair growth liquid comprises dermal papilla cell-derived exosome nanoparticles, a stabilizer, a humectant, a scalp absorption promoter, an antioxidant and water. According to the hair growth liquid, the exosome nanoparticles derived from the dermal papilla cells areused as main functional components, and a stabilizer, a humectant, a scalp absorption promoter, an antioxidant and the like are matched, so that the efficacy of the exosome nanoparticles derived fromthe dermal papilla cells can be maximized. According to the invention, the exosome is freeze-dried into powder, and then the powder is made into nano-particles to be applied to the hair growth liquid;according to the invention, high transdermal rate, good stability and good technical effect can be obtained through normal use; and the stabilizer and the scalp absorption promoter are added so as toprovide the synergistic effect with the exosome nanoparticles derived from the dermal papilla cells in increasing the transdermal rate and the stability. Compared with the common drug minoxidil in the prior art, the hair growth liquid has equivalent or better effects and high bio-safety.

The invention relates to an external use coating agent for treating alopecia. Blood supply of the hair papilla can be increased; the local nutrition is improved; the dual functions of treatment and prevention on hair caring, hair development and the like are accordingly achieved; the external use coating agent has the functions of nourishing blood and essences, tonifying kidney and benefiting liver; the external use coating agent has the unique treatment effect on alopecia caused by essence and blood depletion; factors of the external use coating agent directly act on the corium layer of the scalp; hair follicle regeneration is activated; metabolism of the scalp is improved; sebum secretion is adjusted; the nutrition needed by hairs is supplemented; and the treatment effect on hair solidification, hair development and hair blackening are accordingly achieved. The external use coating agent comprises the following traditional Chinese medicines including folium mori, hemp fimble leaves, cacumen biotae, sesame peduncle, glossy privet fruits, polygonum multiflorum, rhizoma zingiberis and broadleaf holly leaves.

The present invention relates to a composition for preventing hair loss and promoting hair regrowth, the composition comprising an ingredient for inducing anagen hair growth and hair growth promotionto promote the growth of hairs. The composition for preventing hair loss and promoting hair regrowth according to the present invention promotes hair regrowth by shortening the period of transition from the telogen stage to the anagen stage and delays the transition to catagen stage in the hair growth cycle, thereby exhibiting an excellent effect of preventing hair loss and promoting hair regrowth. In addition, the composition of the present invention is safe to the human body, with no side effects thereto, promotes the proliferation of dermal papilla cells, and has an excellent effect of increasing hair elasticity.

With an object of discovering a substance having a testosterone 5α-reductase inhibiting action, a hair papilla cell growth promoting action, a fibroblast growth factor-7 production promoting action, a vascular endothelial growth factor production promoting action or a bone morphogenetic protein-2 production promoting action, and of providing a hair care product having this substance blended therein, and a hair growth tonic, a testosterone 5α-reductase inhibitor, a hair papilla cell growth promoter, a fibroblast growth factor-7 production promoter, a vascular endothelial growth factor production promoter and a bone morphogenetic protein-2 production promoter each having this substance as an active ingredient thereof, a hair care product of the present invention is made to contain corosolic acid therein, and each of a hair growth tonic, a testosterone 5α-reductase inhibitor, a hair papilla cell growth promoter, a fibroblast growth factor-7 production promoter, a vascular endothelial growth factor production promoter and a bone morphogenetic protein-2 production promoter of the present invention is made to contain corosolic acid as an active ingredient thereof.

The invention discloses a novel externally applied traditional Chinese medicine composition for treating alopecia areata. The traditional Chinese medicine comprises the following raw materials in portion by weight: 2 to 5 portions of full cantharis, 8 to 12 portions of Davallia mariesii and 45 to 55 portions of distilled spirit. As proved by clinical observation results, the novel externally applied traditional Chinese medicine composition has obvious effect of treating alopecia areata caused by malnutrition of hair papilla, local deficiency of nutritive elements, and the like and alopecia caused by traumas such as scars, sores, scalds and acupuncture. The novel externally applied traditional Chinese medicine composition has the advantages of simple structure, low cost, safety and reliability, convenient use, obvious effect and disease eradication, is accepted by patients, and is one hot spot of the current alopecia areata treatment. Moreover, the cure rate is approximately 95 percent after application treatment of 120 alopecia areata patients for more than three years.