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Method for preparing artificial skin containing hair follicle

A technology of artificial skin and hair follicles, applied in the fields of biomedicine and artificial skin preparation, can solve the problems of no epidermis, easy infection, small culture area, etc., and achieve the effects of promoting normal healing, easy large-scale production, and convenient operation

Inactive Publication Date: 2005-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the artificial skin that is clinically suitable for large-area wound coverage is mainly based on natural materials such as collagen as the scaffold. This type of artificial skin has the disadvantages of small culture area, low yield, easy shrinkage, and low success rate of transplantation; Synthetic materials are scaffolds. Although they are easy to produce, they are prone to infection, and the success rate of transplantation is low. There is no epidermis, which is not conducive to cell growth. The research of artificial skin focuses on the improvement of matrix and anti-infection ability. There are few researches on ingredients. Although artificial skin containing dermis and epidermis has been successfully developed at home and abroad in recent years, and has certain anti-infection ability, so far there has been no report of artificial skin containing hair follicles.

Method used

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  • Method for preparing artificial skin containing hair follicle
  • Method for preparing artificial skin containing hair follicle
  • Method for preparing artificial skin containing hair follicle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Use microsurgical scissors and microsurgical photography to separate a number of mouse vibrissae hair follicles (>100) under a dissecting microscope, add 0.5% dissociating enzyme, digest for 16-18 hours at 4°C, and transfer the hair follicles into D-Hanks solution, centrifuged to remove the isolated enzyme. Squeeze out the hair shaft from the hair follicle, digest the hair follicle epithelial cells with 0.125% trypsin plus 0.02% EDTA, stop the digestion with 5% D-Hanks solution of newborn bovine serum, blow the cells, sieve, count, and centrifuge.

[0018] (2) Digest rat vibrissa follicles with 0.3% collagenase D at 37°C for 4 to 6 hours after removal of the hair shaft, and stop when the dermal sheath surrounding the hair follicles has been digested into single cells and the dermal papilla inside has not yet begun to digest Digest, wash with D-Hanks solution, centrifuge to remove collagenase D three times, centrifuge at low speed (300rpm) for 5 minutes, collect supe...

Embodiment 2

[0021] The preparation method refers to Example 1, and the subcultured dermal papilla cells (4 / ml. A small amount of hair follicle-like structures were observed after 8 weeks of culture.

Embodiment 3

[0023] The preparation method refers to Example 1, and the subcultured dermal papilla cells (5 / ml. A small amount of hair follicle-like structures were observed after 8 weeks of culture.

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Abstract

The present invention relates to a preparation method of artificial skin containing hair follicle. Said method includes the following steps: separating mouse palp hair follicle, adding resolvase to make digestion, centrifugalizing to obtain hair follicle epithet lial cell, taking out mouse palp hair follicle whose hair shaft is removed, using collagenase D to make digestion, centrifugalizing, using DMEM culture medium to make dermal sheath cell culture and make hair papilla cell culture; making the subcultured cell be respectively mixed with hair follicle epithelial cell according to the ratio of 5-1:1-5, and transplanting on collage / chtosan porous support, adding DMEM culture medium to make culture so as to obtain the invented product.

Description

technical field [0001] The invention belongs to biomedicine and relates to a preparation method of artificial skin, in particular to a preparation method of artificial skin containing hair follicles. Background technique [0002] Recently, artificial skins containing dermis and epidermis have been successfully developed at home and abroad, but these artificial skins have no other cell components, such as no sebaceous gland cells, and the product secreted by sebaceous glands is called sebum, which has the effect of lubricating the skin and forms on the skin surface Fatty membrane, which forms part of the skin barrier. The free fatty acids in the lipid film can inhibit the growth of some pathogenic microorganisms; the fibroblasts in the dermal tissue around the hair follicles can produce natural antibiotics and have antibacterial defense capabilities. The outer hair root sheath in the hair follicle is equivalent to the spinous layer and basal layer cells of the epidermis, whi...

Claims

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Application Information

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IPC IPC(8): A61L27/38
CPCA61L27/60A61L2430/18
Inventor 吕中法郑敏蔡绥勍马列吴贤杰高长友
Owner ZHEJIANG UNIV
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