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Hair restoring liquid containing dermal papilla cell derived exosome and collagen and preparation method thereof

A dermal papilla cell and collagen technology, which is applied in the field of hair growth liquid containing dermal papilla cell-derived exosomes and collagen and its preparation field, can solve the problem of inability to treat both the symptoms and root causes, difficulty in lifting finasteride, poor effect, etc. It can improve the structural stability and long-term efficacy, promote proliferation activity and migration activity, and improve the effect of oily scalp and hair loss.

Pending Publication Date: 2020-09-04
南京元荟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are limitations in traditional hair loss treatment methods: for example, drug treatment is not effective for some people, and finasteride may cause side effects such as difficulty in lifting and impotence; surgical treatment is limited to the follicular unit of the donor area, and it is not suitable for end-stage hair loss. helpless
[0007] Although the hair care products disclosed in the prior art have a certain effect on activating hair follicles and promoting hair growth, the effect is still very limited, and the effect of treating both symptoms and root causes cannot be achieved.

Method used

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  • Hair restoring liquid containing dermal papilla cell derived exosome and collagen and preparation method thereof
  • Hair restoring liquid containing dermal papilla cell derived exosome and collagen and preparation method thereof
  • Hair restoring liquid containing dermal papilla cell derived exosome and collagen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0071] Isolation and culture of human scalp dermal papilla cells:

[0072] (1) The discarded human scalp hair follicles after hair transplantation (with the patient's signature and consent), were immediately placed in a sterile centrifuge tube filled with Bingsheng Ringer's solution, and quickly transferred to the laboratory. Human scalp tissue needs to separate the hair follicles under a microscope, cut the hair bulbs with micro scissors and move them to a new PBS petri dish.

[0073] (2) Collect all excised dermal papillae and wash them three times with fresh PBS. Use a syringe to carefully aspirate the PBS washing solution under the microscope, taking care not to aspirate the dermal papilla. Digest the dermal papilla with 0.2% type I collagenase, and digest it in a cell culture incubator at 37°C for 1.5 hours. Under the microscope, it can be seen that some black dermal papilla cells are separated from the white dermal sheath. At this time, pipette Pipette with a gun and c...

preparation example 2

[0077] Extraction of exosomes from low-generation human dermal papilla cells:

[0078] When the 3rd or 5th generation human scalp dermal papilla cells are within 25cm 2When the growth in the culture bottle reaches 90% confluence, replace the culture medium with 5 mL low-sugar DMEM medium containing no fetal bovine serum and culture for 3 days, then transfer it to a special centrifuge tube of 50 mL high-speed centrifuge, centrifuge at 2000 g at 4 °C for 20 min. Remove the supernatant and transfer it to a polyethylene glycol tube suitable for ultracentrifugation rotors. Mark one side of each ultracentrifuge tube with a waterproof marker, place the tube on the rotor with the marked side up, and centrifuge at 10,000 g, 4 °C for 30 min, then transfer the supernatant to the same size polyethylene glycol as above centrifuge at 4°C for 1 h at 100,000 g, remove the supernatant thoroughly, and resuspend the pellet in each tube in 1 mL of PBS using a micropipette.

[0079] The exosomes...

Embodiment 1

[0081] This embodiment provides a hair tonic, which includes an exosome composition and auxiliary materials. The exosome composition is dermal papilla cell-derived exosomes and collagen coated therein at a mass ratio of 7:1; the auxiliary material and hair papilla The mass ratio of cell-derived exosomes is 20:1; the excipients are 2 parts of stabilizer (1 part of sodium hyaluronate, 1 part of hydroxyethyl starch), 3 parts of humectant (propylene glycol), and 2 parts of scalp absorption promoter ( 1 part of dimethyl sulfoxide, 1 part of menthol), 1 part of antioxidant (citric acid) and 100 parts of water.

[0082] The preparation method of this hair tonic is as follows:

[0083] (1) Mix the third-generation dermal papilla cell-derived exosomes prepared in Preparation Example 2 with the electrotransfection buffer to prepare the first solution;

[0084] (2) mixing the first solution obtained in step (1) with collagen, and performing electroporation transduction to obtain a secon...

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Abstract

The invention relates to hair restoring liquid containing a dermal papilla cell derived exosome and collagen and a preparation method thereof. The hair restoring liquid comprises an exosome composition and accessories; and the exosome composition comprises the dermal papilla cell derived exosome and the collagen coated in the dermal papilla cell derived exosome. Main functional components of the hair restoring liquid to which the invention relates are the dermal papilla cell derived exosome and the collagen coated in the dermal papilla cell derived exosome; the dermal papilla cell derived exosome and the collagen supplement each other and take synergetic effects; proliferation activity and migratory activity of hair matrix cells can be promoted; hair oiliness and the baldness phenomenon ofa patient suffering from serious baldness can be obviously improved; and growth of restored hair is promoted.

Description

technical field [0001] The invention belongs to the technical field of biological cells, and relates to a hair tonic and a preparation method thereof, in particular to a hair tonic containing dermal papilla cell-derived exosomes and collagen and a preparation method thereof. Background technique [0002] Hair follicle is a kind of complex tiny organ. The formation and occurrence of hair follicle is the result of the interaction between hair follicle epithelial and dermal components. The growth and regeneration of hair follicle is regulated by its own stem cells. Stem cells in hair follicles mainly include: hair follicle stem cells (HFSCs), dermal papilla cells (DPCs) and melanocyte stem cells (McSCs). Among them, DPCs and HFSCs play an important role in hair follicle morphogenesis. [0003] Exosomes are cell secretion products, which are tiny vesicles with a lipid bilayer membrane with a diameter of about 50-15nm surrounded by regulatory proteins, mRNA, miRNA and other stru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/39A61K9/50A61K47/46A61P17/14A61P17/00A61K35/36
CPCA61K38/39A61K9/5068A61K35/36A61P17/14A61P17/00A61K2300/00
Inventor 卢俊锋游同钊
Owner 南京元荟生物科技有限公司
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