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Hair growth liquid containing dermal papilla cell-derived exosome and preparation method thereof

A technology of dermal papilla cells and exosomes, applied in the field of biological cells, can solve the problems of finasteride lifting difficulty, poor effect, and limited surgical treatment, so as to promote proliferation activity and migration activity, and improve scalp oily feeling And hair loss phenomenon, the effect of high biosafety

Pending Publication Date: 2020-08-18
南京元荟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are limitations in traditional hair loss treatment methods: for example, drug treatment is not effective for some people, and finasteride may cause side effects such as difficulty in lifting and impotence; surgical treatment is limited to the follicular unit of the donor area, and it is not suitable for end-stage hair loss. helpless
Although, in other fields, the therapeutic effect of exosomes derived from stem cells has been widely reported, in the field of skin, this research is still relatively lacking.

Method used

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  • Hair growth liquid containing dermal papilla cell-derived exosome and preparation method thereof
  • Hair growth liquid containing dermal papilla cell-derived exosome and preparation method thereof
  • Hair growth liquid containing dermal papilla cell-derived exosome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0058] Isolation and culture of human scalp dermal papilla cells:

[0059] (1) The discarded human scalp hair follicles after hair transplantation (with the patient's signature and consent), were immediately placed in a sterile centrifuge tube filled with Bingsheng Ringer's solution, and quickly transferred to the laboratory. Human scalp tissue needs to separate the hair follicles under a microscope, cut the hair bulbs with micro scissors and move them to a new PBS petri dish.

[0060] (2) Collect all excised dermal papillae and wash them three times with fresh PBS. Use a syringe to carefully aspirate the PBS washing solution under the microscope, taking care not to aspirate the dermal papilla. Digest the dermal papilla with 0.2% type I collagenase, and digest it in a cell culture incubator at 37°C for 1.5 hours. Under the microscope, it can be seen that some black dermal papilla cells are separated from the white dermal sheath. At this time, pipette Pipette with a gun and c...

preparation example 2

[0064] Extraction of exosomes from low-generation human dermal papilla cells:

[0065] When the 3rd or 5th generation human scalp dermal papilla cells are within 25cm 2 When the growth in the culture bottle reaches 90% confluence, replace the culture medium with 5 mL low-sugar DMEM medium containing no fetal bovine serum and culture for 3 days, then transfer it to a special centrifuge tube of 50 mL high-speed centrifuge, centrifuge at 2000 g at 4 °C for 20 min. Remove the supernatant and transfer it to a polyethylene glycol tube suitable for ultracentrifugation rotors. Mark one side of each ultracentrifuge tube with a waterproof marker, place the tube on the rotor with the marked side up, and centrifuge at 10,000 g, 4 °C for 30 min, then transfer the supernatant to the same size polyethylene glycol as above centrifuge at 4°C for 1 h at 100,000 g, remove the supernatant thoroughly, and resuspend the pellet in each tube in 1 mL of PBS using a micropipette.

[0066] The exosome...

Embodiment 1

[0068] This embodiment provides a hair tonic, which includes 5 parts of low-generation dermal papilla cell-derived exosome nanoparticles, 2 parts of stabilizer (1 part of sodium hyaluronate, 1 part of carboxymethyl chitosan), moisturizer ( Propylene glycol) 3 parts, scalp absorption accelerator 2 parts (dimethyl sulfoxide 1 part, menthol 1 part), antioxidant 1 part (citric acid) and water 100 parts.

[0069] Among them, the preparation method of low-generation dermal papilla cell-derived exosome nanoparticles is as follows:

[0070] (1) Freeze-drying the third-generation dermal papilla cell-derived exosomes prepared in Preparation Example 2 to make dermal papilla cell-derived exosome powder;

[0071] (2) Mix the dermal papilla cell-derived exosome powder obtained in step (1) with dioctyl carbonate, and the concentration of the dermal papilla cell-derived exosome powder in dioctyl carbonate is 25 mg / mL to prepare oil phase solution;

[0072] (3) Glycyrrhizin, cocamidopropyl b...

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Abstract

The invention relates to a hair growth liquid containing a dermal papilla cell-derived exosome and a preparation method thereof. The hair growth liquid comprises dermal papilla cell-derived exosome nanoparticles, a stabilizer, a humectant, a scalp absorption promoter, an antioxidant and water. According to the hair growth liquid, the exosome nanoparticles derived from the dermal papilla cells areused as main functional components, and a stabilizer, a humectant, a scalp absorption promoter, an antioxidant and the like are matched, so that the efficacy of the exosome nanoparticles derived fromthe dermal papilla cells can be maximized. According to the invention, the exosome is freeze-dried into powder, and then the powder is made into nano-particles to be applied to the hair growth liquid;according to the invention, high transdermal rate, good stability and good technical effect can be obtained through normal use; and the stabilizer and the scalp absorption promoter are added so as toprovide the synergistic effect with the exosome nanoparticles derived from the dermal papilla cells in increasing the transdermal rate and the stability. Compared with the common drug minoxidil in the prior art, the hair growth liquid has equivalent or better effects and high bio-safety.

Description

technical field [0001] The invention belongs to the technical field of biological cells, and relates to a hair tonic and a preparation method thereof, in particular to a hair tonic containing exosomes derived from dermal papilla cells and a preparation method thereof. Background technique [0002] Hair is a symbol of personal beauty and plays a vital role in everyday life. However, with the increasing pressure of modern life, study and work, more and more people suffer from hair loss. Among all the causes of hair loss, androgenic alopecia (AGA) is the most common. At present, the means for the treatment of hair loss mainly include drug therapy and surgical treatment. Among them, the FDA-approved drugs for the treatment of androgenetic alopecia are oral finasteride and topical minoxidil. Surgical treatment is hair transplantation, which can be divided into follicular unit transplantation and follicular unit extraction transplantation according to the operation method. Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/73A61K8/49A61K8/19A61K8/34A61K8/365A61K8/67A61K8/44A61K8/63A61Q7/00A61P17/14
CPCA61K8/99A61K8/735A61K8/736A61K8/732A61K8/4906A61K8/19A61K8/34A61K8/345A61K8/365A61K8/676A61K8/442A61K8/63A61Q7/00A61P17/14A61K2800/84A61K2800/592A61K2800/522A61K2800/52
Inventor 卢俊锋游同钊
Owner 南京元荟生物科技有限公司
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