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Rice zinc finger protein gene for negative regulating plant apoptosis and promoting growth and differentiation of transgene callus

A zinc finger protein and gene technology, applied in the field of plant molecular biology, can solve the problems of long time-consuming, only observation of specific developmental stages of cells, cumbersome experimental steps, etc.

Inactive Publication Date: 2003-10-29
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has disadvantages such as cumbersome experimental steps, long time-consuming, difficult operation, and can only observe specific developmental stages of cells.

Method used

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  • Rice zinc finger protein gene for negative regulating plant apoptosis and promoting growth and differentiation of transgene callus
  • Rice zinc finger protein gene for negative regulating plant apoptosis and promoting growth and differentiation of transgene callus
  • Rice zinc finger protein gene for negative regulating plant apoptosis and promoting growth and differentiation of transgene callus

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Experimental program
Comparison scheme
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Embodiment Construction

[0042] The present invention will be described in further detail below through specific embodiments and in conjunction with the accompanying drawings.

[0043] NB 0

N 6 lots of salt, B 5 trace salt and B 5 Organic, 300mg / L casein hydrolyzate,

500mg / L proline, 30g / L sucrose, 3g / L phytagel, pH5.8

NBD

NB 0 Medium, 2.0mg / L 2.4-D, pH5.8

AAM

N 6 Medium, 2mg / L 2.4-D, 1g / L casein hydrolyzate, 30g / L cane

Sugar, 10g / L glucose, 100μM acetosyringone, pH5.2

NA

N 6 Medium, 2mg / L 2.4-D, 1g / L casein hydrolyzate, 30g / L cane

Sugar, 10g / L glucose, 100μM acetosyringone, pH5.2

NBDH

NBD, 50mg / L hygromycin, 250mg / L cephalosporin, 200mg / L

Ampicillin, pH 5.8

NBP

NB 0 Medium, 1.0mg / L 6-BA, 2.0mg / L NAA, 5.0mg / L ABA,

50mg / L hygromycin, pH5.8

NBR

NB 0 Medium, 2.0mg / L 6-BA, 1.0mg / L NAA, 1.0mg / L KT, 1.0

mg / L IAA, 50mg / L hygromycin, pH5.8

1 / 2MS

2.15g / L MS (GIBCO), B ...

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Abstract

A zinc finger protein gene OsLSD1 of paddy rice contains 3 zinc finger structure domains and can code a protein containing 143 amino acids. Its expression is fully suppressed by rice blast bacteria. Said OsLSD1 protein is a negative regulatory factor of programmed cell death and can promote the growth differentiation of transgenic celli of rice. The transgenic tobacco expressing OsLSD1 has high tolerance to cancerogenic microbial toxin fumonisin B1 (FB1).

Description

technical field [0001] The invention belongs to the field of plant molecular biology. Specifically, the present invention relates to obtaining a rice cDNA homologous to the Arabidopsis thaliana LSD1 (lesions simulating disease resistance 1) gene by sequencing and analyzing the rice cDNA library, named OsLSD1 (Oryza sativahomolog of LSD1). The cDNA contains a 432bp open reading frame encoding 143 amino acids. The protein contains three zinc finger domains, similar to the Arabidopsis LSD1 protein. The results of Northern hybridization showed that the expression of the gene was completely inhibited by Magnaporthe grisea. Three plant expression vectors pCOsLSD1-S, pCOsLSD1-A, pCOsLSD1-R were constructed, and the transgenic rice results showed that OsLSD1 gene is a negative regulator of plant programmed cell death and can promote the growth and differentiation of transgenic callus. Transgenic tobacco expressing OsLSD1 has increased tolerance to the oncogenic microbial toxin fumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H5/00C07K14/415C12N15/29C12N15/63C12N15/70C12N15/74
Inventor 何朝族王丽娟裴忠友
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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