53 results about "Microbial toxins" patented technology

Disposable absorbent articles comprising an absorbent material and a degradable thermoplastic polymer composition comprising an aliphatic polyester and an antimicrobial composition. The antimicrobial composition includes an antimicrobial component and an enhancer component. The aliphatic polyester and antimicrobial composition are formed into webs by melt extrusion, such as nonwovens and films, that are incorporated into disposable absorbent articles, such as disposable infant diapers, adult incontinence articles, feminine hygiene articles such as sanitary napkins, panty liners and tampons, personal care wipes and household wipes to provide odor control, control of microbial growth, and control of microbial toxin production.

The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.

Three choline utilizing strains of microorganisms isolated from the anthers of wheat, Aureobasidium pullulans strain AS 55.2, Arthrobacter species strain OH 221.3, and Pseudomonas species strain AS 64.4, are superior antagonists of F. graminearum. These microorganisms are effective for suppression and control of FHB in cereals, particularly in wheat and barley.

The invention relates to a barton fermentation bed complex microbial inoculant and a preparation method thereof. The invention is characterized in that the complex microbial inoculant comprises the following six beneficial microorganisms in percentage by weight: 15-30 percent of Trichoderma koningii, 10-25 percent of geotrichum candidum, 10-25 percent of Candida utilis, 10-30 percent of lactobacillus plantarum, 10-25 percent of bacillus subtilis and 5-15 percent of bacillus subtilis natto. The complex microbial inoculant is prepared by a fermentation process, and the total number of fermentation viable bacteria in finished products is 2-5*10<9>cfu/ml. The microbial strains adopted by the complex microbial inoculant are safe and reliable, the finished viable bacteria content is high and the bioactivity is high; 1L/m<3> complex microbial inoculant is used on a barton fermentation bed, thus obviously reducing the odor in a barton, decreasing the breeding of mosquitoes and flies, having stronger degradation capability on chicken excrement, achieving the ecologically breeding purposes of no pollution and zero emission, enhancing the digestive absorption function of chicken, improving the chicken immunity, enhancing the broiler quality, increasing the quality of eggs, and ensuring the color of yolks to be brightly red.

The invention provides a microbial deodorant and strain for eliminating waste odor of municipal landfill and waste transfer station, and the preparation and application method thereof. The preparation method comprises the following steps: culturing Saccharomyces cerevisiae with the conservation number of CGMCC No.5183 in a PDA culture medium, and culturing Lactobacillus casei (CGMCC No.5182) in an MRS culture medium to obtain the seed liquid; and fermenting in a fermentation tank to obtain the microbial deodorant. The microbial deodorant is prepared by a mixed culture manner so as to lower the fermentation cost, reduce the generation of mixed bacteria and stabilize the product quality. The microbial deodorant prepared by the method can effectively eliminate the waste odor of the municipallandfill so as to achieve good deodorant effect.

The invention provides a method for culturing a microbial strain for degrading industrial waste water, which belongs to the field of biotechnology. The method can solve the problems that the existing strain for degrading the industrial waste water has single degradation role and can not degrade compound pollutants. The method for culturing the microbial strain for degrading the industrial waste water comprises the following steps: preparing liquid culture media containing different concentration gradients of target compounds; obtaining a plasmid-containing strain which takes an aniline compound or a nitrobenzene compound as a sole carbon and nitrogen source and takes phenol as a sole carbon source respectively; carrying out acclimatization, culture and identification of plasmid and degradation capability; and purifying for obtaining the plasmid-containing microbial strain for degrading the industrial waste water. The culture method has the advantages of simple process and low cost of the used raw materials, and the cultured strain has the capability of degrading the compound pollutants and has higher efficiency for degrading aniline, phenol and nitrobenzene compounds.

The invention discloses a microbial strain and an application thereof, particularly relates to a tobacco nicotine degrading microbacterium GYC29 (Microbacterium sp.GYC29) with CCTCC of NO:M2010311 and an application of the microbacterium in tobacco. The strain disclosed herein can decompose and utilize nicotine in the growth process. According to the invention, by adding the ferment liquor of the strain or thallus with the additional amount of 1-5 % of the weight of the tobacco leaves in the tobacco with the water content of 10-50 % and fermenting for 6-72 h, the nicotine content of the tobacco leaves is reduced by 2-33%, the irritation of the tobacco leaves is obviously reduced, the miscellaneous gases are reduced, the smoke becomes pure and mild, and the sensory quality is obviously improved; the degradation of tobacco nicotine is realized by using microbes, the nicotine content of tobacco raw materials can be regulated properly, and the applicability of the tobacco leaves is raised.

The invention relates to microbial fertilizer prepared by bacillus laterosporus and a preparation method of the microbial fertilizer. The fertilizer is prepared by culturing a microbial strain with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 5090 to form a culture, mixing the culture and grass carbon powder to form a microbial strain agent and mixing the microbial strain agent and conventional fertilizer. A culture method of the microbial strain culture comprises the steps of: 1) inoculating a volume of strain suspension to 20 volumes of No. 1 culture medium for aerobic culture for 8h at 30-38 DEG C, 2) adding a culture solution obtained in Step 1) into about 20 volumes of No. 2 culture medium for aerobic culture for 8h at 30-38 DEG C, and 3) adding a culture solution obtained in Step 2) into about 20 volumes of No. 3 culture medium for culture for 8h at 35-38 DEG C to produce the culture.

The present invention is related to compositions and methods to treat, ameliorate and/or prevent morbidity and/or mortality from microbial infections. In particular, bacterial infections that are associated with the production and release of bacterial toxins. For example, many Clostridia bacteria, such as Clostridium difficile, release toxins resulting in tissue and organ damage and death, even after antibiotic therapy that either reduces or eliminates the bacteria. In particular, various peptides, polypeptides, and proteins are disclosed herein that either inactivate Clostridium difficile toxin and/or reduce Clostridium difficile toxin production.

The invention provides a screening method for a feather degradation strain. The specific steps include: soil sample taking from a chicken farm site for accumulating chickens died of illness; enrichment; preliminary screening; secondary screening; separation and purification: observing the growth condition of microbial strains on a solid screening plate, selecting a dominant strain growing best, and selecting single colony for plate streaking; conducting streak culture to obtain the single colony, i.e. feather degradation strain ABTNL-4. The invention also provides an application method of the feather degradation strain, and the method includes: taking feather powder as the only carbon source and nitrogen source to prepare a fermentation medium, adding the feather degradation strain ABTNL-4 into the medium, and performing culture at 37DEG C, thus realizing feather degradation. The method provided by the invention can achieve a feather degradation rate up to 95%, compared with the physical-chemical process for feather degradation, the microbial method for feather degradation has the advantages of mild reaction conditions, little energy consumption, economical efficiency and environmental protection, at the same time avoids the loss of most amino acids, and improves the absorbability and utilizability feather degradation products.

The invention discloses anti-acarus and anti-microbial non-woven fabric containing a natural plant extract. The non-woven fabric comprises three layers, wherein the upper layer is an anti-acarus non-woven fabric layer, the lower layer is an anti-microbial non-woven fabric layer, and the middle layer is a bonding layer; the anti-acarus non-woven fabric layer is obtained through a hydroentanglementprocess by adopting chitin fiber, and the anti-microbial non-woven fabric layer is boned with the anti-acarus non-woven fabric layer through the bonding layer. A preparation method of the anti-microbial non-woven fabric layer comprises the following steps: (1) adding Houttuynia cordata, honeysuckle, radix bupleuri, Astragalus membranaceus, Coptis chinensis, camphor, mint, radix sileris and Poria cocos into water for boiling for 2 to 3 h, filtering to obtain a filter residue for standby use, and thus obtaining a medicine liquor A; (2) adding the filter residue in the step (1) into water for boiling for 2 to 3 h, filtering, and abandoning the filter residue to obtain a medicine liquor B; (3) merging the medicine liquor A and the medicine liquor B in the step (1) and the step (2), and then adding water for dilution to obtain a traditional Chinese medicine solution; and (4) soaking the non-woven fabric into the traditional Chinese medicine solution in the step (3) for 20 to 24 h, and carrying out low temperature drying to obtain the anti-microbial non-woven fabric.

The present invention relates to the Rhodosporidium toruloides CECT 13085 strain, as well as to uses thereof for obtaining microbial biomass rich in triglycerides and for producing oils of a microbial origin in the presence of lignocellulosic biomass hydrolysates.

The invention belongs to the field of molecular detection for microbial toxin in food science and technology, in particular to rapid detection and parting of 11 serotypes of staphylococcal enterotoxin in food by applying the flows such as total DNA component extraction and SYBRGreen fluorescent quantitative PCR (polymerase chain reaction) rapid detection. Results show that the staphylococcal enterotoxin in food can be effectively typed according to the situation whether marked amplification curves appear in 35 PCR cycles by utilizing the method, thus the method has the characteristics of specificity aiming at enterotoxin genes of different serotypes, sensitivity, accuracy, rapidness and convenience and the like; and compared with the existing method, the method provided by the invention has the advantages of greatly improving the detection and parting efficiency and providing a new way for risk analysis of food pollution caused by the staphylococcal enterotoxin of different types.

A composition of chemically purified soluble mannans from legumes' seeds (e.g. Ceratonia siliqua, Caesalpinia spinosa Trigonelle foenum-graecum, and Cyamopsis tetragonolobus) and their use in the assembly of palatable dietary supplements is disclosed herein. The fractionation process provides high-quality physiologically soluble, chemically modified and purified homogeneous size polysaccharide fibers, devoid of natural impurities, for example proteins, alkaloids, glycoalkaloids, and/or environmental impurities including heavy metals, agricultural residues and microbial toxins. This process provides hypoallergenic dietary fibers devoid of any potential allergens, cytotoxins, and gastrointestinal toxins. A sequential process for assembly of the soluble fibers with plurality of molecular weights to create a time controlled dissolution of the functional high and low molecular weight fibers for improving solubility and palatability with improved dietary performance in the oral and gastro-intestinal system is also disclosed herein.

The invention relates to bacillus velezensis BL-14 which is subjected to mutagenesis screening and a liquid mixed bacterium solution of the bacillus velezensis BL-14, wherein the liquid mixed bacterium solution is used for efficiently degrading deoxynivalenol (DON), aflatoxin (AFB1) and zearalenone (ZEN) in feed raw materials. The strain BL-14 (CGMCC No.22083) has a rich hydrolase system, can produce amylase, protease, cellulase and the like, can be combined with lactobacillus plantarum 1.2158 (CGMCC) and bacillus coagulans 1.6565 (CGMCC) to form a mixed bacterial agent for feed raw material fermentation, can degrade 83.25% of vomitoxin, 90.09% of aflatoxin (AFB1) and 80.76% of zearalenone in the raw materials, has the lactic acid content of 36.5 g/Kg in the fermented feed, and has the advantages that the lactic acid content of the fermented feed reaches 36.5 g/Kg; the feed quality is excellent.

A wine making process adding microbial strains includes: selecting glutinous hard red granular sorghum rice, high-quality mature full rice, and full-grain sun-dried waxy corn; crushing the sorghum rice into sorghum particles, the rice into rice particles and the waxy corn into waxy corn particles; mixing 35-50 Kg of the sorghum particles, 30-40 Kg of the rice particles and 8-15 Kg of the waxy corn, and soaking; steaming the soaked materials; adding 0.5-0.8 Kg of Rhizopus and 0.6-1 Kg of Monascus into the steamed mixed cereals for primary fermentation; after primary fermentation, placing in a cellar, and adding 0.2-0.4 Kg of Schizosaccharomyces, 0.4-0.6 Kg of aroma-producing yeast and 0.6-0.8 Kg of Lactobacillus for cellar fermentation. The wine making process adding microbial strains provides reasonable burdening of wine-making materials, wine-making steps are effectively controlled, and the different microbial strains are added, providing palatability.

Described herein is a microbial composition used to enhance anti-cancer drugs. Specifically, microbial compositions that comprise a part of or an entire microorganism having surface lectins specific to carbohydrate moieties on tumor surface combined with an oncolytic agent.

The invention provides a preparation method of a composite strain bio-organic fertilizer. The preparation method comprises the following steps: (1) screening and separating composite microbial strain;(2) key process technology research on inoculation between the composite microbial agent and an organic fertilizer; (3) formulating a product quality standard of the bio-organic fertilizer; and (4) performing industrialization production of the bio-organic fertilizer. By selecting appropriate microbes, the special microbial strain special for the organic fertilizer is screened, the high-efficiency and environmental-friendly bio-organic fertilizer is further cultured, the microbial strain for organic matter fermentation is provided for microbial fertilizer production enterprises, agriculturalplanting cooperation and vast peasants in China, waste materials are changed into things of value, sustainable development of agriculture in China is realized, the quality and safety of agricultural products are improved, the planting environment is improved, environmental benefits are realized, and huge effects are achieved. The project accords with goals of national 'circular economy' and 'low carbon economy'. The project implementation has obvious economic benefits, social benefits and ecological benefits.