Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer
A microbial fertilizer, microorganism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of defective cultivation methods, high prices, and low yields
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[0047] Culture method of the present invention
[0048]Step 1. First, culture and activate the bacteria on a slant, and then rinse the slant with 5ml sterile water to form a bacterial suspension.
[0049] Step 2. Put the bacterial suspension into a 500ml Erlenmeyer flask, which contains 100ml No. 1 medium, and cultivate it on a shaker for 8 hours. The culture conditions are 37°C, 180-200rpm,
[0050] Step 3. Transfer 105ml of the bacterial solution in the 500ml Erlenmeyer flask into a 5000ml Erlenmeyer flask, which contains 2100ml of No. 2 medium, and cultivate it on a shaker for 8 hours at 37°C, 180-200rpm;
[0051] Step 4. Inject 2205ml of bacterial solution in the 5000ml triangular flask into 60m 3 In the fermenter, the fermenter contains 44.1 L of medium No. 3, and the fermentation conditions are: temperature 36-38° C., tank pressure 0.5 kg / cm, ventilation rate 20 m3 / h, and culture for 8 hours; the culture is obtained.
[0052] Detect the bacterial cell concentration, th...
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