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Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer

A microbial fertilizer, microorganism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of defective cultivation methods, high prices, and low yields

Inactive Publication Date: 2016-01-27
北京鑫鑫盛达生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The Chinese patent discloses a microbial strain with a preservation number of CGMCCNo.5090, but it is found through experiments that the cultivation method has defects, low yield and high price. In order to increase the yield, the inventor improved the cultivation method, unexpectedly Obtaining a high-yield preservation number of microbial strain cultures of CGMCCNo.5090 greatly reduces the price of fertilizers and is conducive to large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Culture method of the present invention

[0048]Step 1. First, culture and activate the bacteria on a slant, and then rinse the slant with 5ml sterile water to form a bacterial suspension.

[0049] Step 2. Put the bacterial suspension into a 500ml Erlenmeyer flask, which contains 100ml No. 1 medium, and cultivate it on a shaker for 8 hours. The culture conditions are 37°C, 180-200rpm,

[0050] Step 3. Transfer 105ml of the bacterial solution in the 500ml Erlenmeyer flask into a 5000ml Erlenmeyer flask, which contains 2100ml of No. 2 medium, and cultivate it on a shaker for 8 hours at 37°C, 180-200rpm;

[0051] Step 4. Inject 2205ml of bacterial solution in the 5000ml triangular flask into 60m 3 In the fermenter, the fermenter contains 44.1 L of medium No. 3, and the fermentation conditions are: temperature 36-38° C., tank pressure 0.5 kg / cm, ventilation rate 20 m3 / h, and culture for 8 hours; the culture is obtained.

[0052] Detect the bacterial cell concentration, th...

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Abstract

The invention relates to microbial fertilizer prepared by bacillus laterosporus and a preparation method of the microbial fertilizer. The fertilizer is prepared by culturing a microbial strain with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 5090 to form a culture, mixing the culture and grass carbon powder to form a microbial strain agent and mixing the microbial strain agent and conventional fertilizer. A culture method of the microbial strain culture comprises the steps of: 1) inoculating a volume of strain suspension to 20 volumes of No. 1 culture medium for aerobic culture for 8h at 30-38 DEG C, 2) adding a culture solution obtained in Step 1) into about 20 volumes of No. 2 culture medium for aerobic culture for 8h at 30-38 DEG C, and 3) adding a culture solution obtained in Step 2) into about 20 volumes of No. 3 culture medium for culture for 8h at 35-38 DEG C to produce the culture.

Description

technical field [0001] The invention relates to a microbial fertilizer prepared from novel Bacillus lateralis and a preparation method thereof, belonging to the field of microbial fertilizers. Background technique [0002] my country has a large population but little arable land, and the arable land decreases year by year with the growth of population and the development of industry and cities. Therefore, the only feasible way to increase the output of agricultural products in our country is to increase the output per unit area. In order to meet the demand for agricultural products from industrial development and population increase, chemical fertilizers have been widely used logically. The long-term unreasonable use of chemical fertilizers has brought [0003] The problems of soil compaction, soil fertility decline, ecological damage, environmental pollution and the decline in the quality of agricultural and sideline products have been highly valued by relevant state depa...

Claims

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Application Information

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IPC IPC(8): C05F11/08C12N1/20C12R1/07
Inventor 麻林涛王莉
Owner 北京鑫鑫盛达生物工程技术有限公司
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