The invention discloses a novel
klebsiella pneumoniae strain as well as an isolation method and application thereof. The novel strain is isolated and screened from
activated sludge in an
aeration tankof a
papermaking sewage treatment
plant and is collected in China General
Microbiological Culture Collection Center, the collection name is ZS-01, the collection number is 16041, and the collection date is June 30, 2018. The strain has the characteristics and performance as follows: (1), the characteristics are: colonies formed on a
phenol MedA
solid culture medium are relatively short and thickbacilli, have the sizes of (0.5-0.8)*(1-2)[mu]m, are arranged separately or in pairs, and are pale yellow, uniform in texture and opaque; relatively large gray white sticky colonies are formed on an MPYE
solid culture medium, are flagella-free, have capsules, belong to
enterobacteriaceae and are
Gram-negative short and thick
bacilli; (2), the performance is: the strain has certain
phenol degradingcapability and has relatively high tolerance to
phenol. The strain has the advantages as follows: the strain can degrade the phenol with relatively
high concentration, and provides a new bacterial source for effectively treating phenol-containing
wastewater with relatively
high concentration.