55 results about "Mucor circinelloides" patented technology

The invention discloses a fluorescence quantitative PCR primer, a probe and a kit for detecting ordinary pathogenic fungi. A specific primer and a TaqMan probe are independently designed, and a fluorescence PCR detection method which can be used for simultaneously detecting 15 clinically ordinary pathogenic fungi, including 8 candida mycoderma bacteria (candida albicans, candida glabrata, candida parapsilosis, candida kefyr, candida sake, candida kruse, candida guilliermind and candida tropicalis), four aspergilli (aspergillus niger, aspergillus flavus, aspergillus terreus and aspergillus fumigates), cryptococcus, rhizopus oryzae and mucor circinelloides, is established. The method is relatively high in sensitivity and specificity, and has significant meanings for early diagnosis and treatment on invasive infections with fungi.

The present invention relates to a microbial agent for treating heavy metal polluted soil, a preparation method and applications thereof. The microbial agent comprises the following raw materials: rice husk, pig manure, chicken manure, soybean powder, single cell protein, a Mortierella alpina fermentation broth, a Trichoderma asperellum fermentation broth, and a Mucor circinelloides fermentation broth. According to the present invention, the microbial agent prepared by mixing Mortierella alpina, Trichoderma asperellum and Mucor circinelloides according to a certain ratio is firstly used, can be used in the complex and changing environment, and can provide effects in the poor soil environment, wherein the functional microbes provide high tolerance to heavy metals, and can maintain activity and perform efficient repair in the heavily heavy metal polluted soil.

The invention relates to a Mucor circinelloides strain with high grease yield and application thereof. The invention discloses a Mucor circinelloides strain WJ11 and application thereof (a method for producing grease by using the strain). The method comprises the following steps: activating the strain to obtain an activated strain solution, carrying out seed culture under sterile conditions to obtain a seed culture solution, carrying out fermentation culture to obtain a fermentation culture solution, filtering, carrying out freeze-drying to constant weight to obtain a grease-containing Mucor circinelloides WJ11 dry strain, grinding, and extracting grease by a chloroform-methanol process. The Mucor circinelloides strain WJ11 has the advantages of high growth rate and high fatty acid content (up to 30-40% of biomass); the fatty acid content is 2-3 times of the control strain; and the biochemical analysis result shows that the Mucor circinelloides strain WJ11 has obviously higher enzyme activity related to fatty acid accumulation than the control group. The Mucor circinelloides strain has very wide application prospects when being used in microbial grease-producing industry and microbial grease accumulation analysis.

The invention relates to a method for preparing laccase by using a mucor circinelloides DK1 strain. The method comprises the following steps: (1) transferring the mucor circinelloides DK1 strain from a PDA slant culture medium into a triangular flask filled with a basic liquid medium, carrying out shaking culture, filtering the fermentation liquor to obtain mycelium, washing the mycelium by using sterile water, scattering, collecting the mycelium in the sterile water to prepare mycelium suspension, and preserving the suspension for later use; (2) putting an enzyme production culture medium into the triangular flask, inoculating the mycelium suspension, carrying out shaking culture, periodically extracting the fermentation liquor in the culture process, centrifuging, and taking the supernatant, thereby obtaining the crude enzyme liquor; and (3) centrifuging the crude enzyme liquor, and taking the supernatant, thereby obtaining the laccase. The method disclosed by the invention is simple in process, is suitable for large-scale industrial production and the like, and the prepared laccase can effectively remove non-cellulose colloid in flax, jute, kenaf or bamboo fibers, has the advantages of high degumming efficiency, short degumming period, high fiber dispersion rate, high refined dried-jute quality and the like and has good application prospects.

The invention discloses an efficient oil-producing filamentous fungus mucor circinelloides Q-531 and an application thereof. The efficient oil-producing filamentous fungus is classified and named as mucor circinelloides Q-531 and is preserved in China Center for Type Culture Collection; the preservation number is CCTCC M2016364; the preservation date is on July 4th, 2016; the preservation address is at Wuhan University of China. According to the invention, a solid state fermentation process is adopted for directly converting mulberry branches into microbial oil; the highest oil yield can reach up to 42.43+ / -4.01mg / gds; the highest cellulase enzyme activity can reach up to 1.39+ / -0.09 FPU / gds. Besides, the highest oil content in fungus cell is 28.8+ / -2.85%. According to the invention, the mucor circinelloides research is utilized to produce oil by fermenting forestry and agricultural residues (mulberry branches) under solid state, so that the waste resources is reasonably utilized, the oil yield is increased, the production cost is reduced and the industrialization is benefited.

The invention discloses an application of Mucor circinelloides in degradation of lambda-cyhalothrin. The invention aims to solve the technical problem that the application of the Mucor circinelloidesin degradation of the lambda-cyhalothrin is not recorded in the prior art. A liquid seed is prepared by using separated Mucor circinelloides, a lambda-cyhalothrin mother liquor is added under a sterile condition to prepare an isolation medium, expanded culture is carried out, and fermentation culture is carried out at a degradation fermentation temperature of 30 DEG C at a rotating speed of 120 rpm or above for 3-5 d. The Mucor circinelloides has a high lambda-cyhalothrin degrading characteristic and has the advantages of simple culture conditions, and rapidness in propagation, the lambda-cyhalothrin degrading rate is 79.7%, the lambda-cyhalothrin degrading capacity of the Mucor circinelloides is used to decompose pesticide residues, and the application has wide application values in the technical field of application of microbial strains.

The invention provides application of mucor circinelloides in gossypol degradation. Aiming at the technical status that in the prior art, there are no records about the application of the mucor circinelloides in the gossypol degradation, and the current status that cottonseed meal is used as the second largest plant protein resource of China, and in order to provide high-degradation-rate and stable-fermentation-property strains for the biological detoxification technology of cottonseed meal, the application of the mucor circinelloides in the gossypol degradation is provided. The mucor circinelloides has the advantages of being high in gossypol degradation rate, simple in culture condition and high in reproduction speed, the gossypol degradation rate is 67.5%, the cottonseed meal is detoxified by using the degradation ability of the strains to gossypol, and the application has a wide application value in the technical field of microbial strain application.

The present invention relates to a microbial agent for treating heavy metal polluted soil, a preparation method and applications thereof. The microbial agent comprises the following raw materials: rice husk, pig manure, chicken manure, soybean powder, single cell protein, a Mortierella alpina fermentation broth, a Trichoderma asperellum fermentation broth, and a Mucor circinelloides fermentation broth. According to the present invention, the microbial agent prepared by mixing Mortierella alpina, Trichoderma asperellum and Mucor circinelloides according to a certain ratio is firstly used, can be used in the complex and changing environment, and can provide effects in the poor soil environment, wherein the functional microbes provide high tolerance to heavy metals, and can maintain activity and perform efficient repair in the heavily heavy metal polluted soil.