Mucor circinelloides capable of promoting root development of salvia miltiorrhiza and tanshinone synthesis and application of mucor circinelloides

A technology of Mucor crimperides and tanshinone, applied in the fields of application, microbe-based methods, chemicals for biological control, etc., can solve the problems of lack of drug sources, ecological damage, slow growth of medicinal plants, etc., and achieve large applications value, promote biosynthesis and accumulation, and promote the effect of Danshen root development

Active Publication Date: 2021-03-30
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on endophytic fungi in medicinal plants is still in its infancy. So far, there are few reports on the effects of endophytic fungal elicitors that can produce plant-derived bioactive compounds on the secondary metabolism of host plants. Isolation of endophytic fungi from medicinal plants bacteria and use them for ferme

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  • Mucor circinelloides capable of promoting root development of salvia miltiorrhiza and tanshinone synthesis and application of mucor circinelloides
  • Mucor circinelloides capable of promoting root development of salvia miltiorrhiza and tanshinone synthesis and application of mucor circinelloides
  • Mucor circinelloides capable of promoting root development of salvia miltiorrhiza and tanshinone synthesis and application of mucor circinelloides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Isolation, cultivation, identification and preservation of Mucor circinelloides (M.circinelloides) DF20:

[0053] Endophytic fungal strain DF20 of the present invention is obtained by the following steps of isolation:

[0054] 1) Select Salvia miltiorrhiza (S. miltiorrhiza) plants of suitable size and healthy growth indoors, cut off the root of Salvia miltiorrhiza with a length of about 5 cm, and remove the soil attached to the root surface as much as possible.

[0055] 2) Rinse the intercepted root segment of Salvia miltiorrhiza with sterilized PBS solution for 2 times and then with sterile water for 2 times. Then soak the root section of Salvia miltiorrhiza in 200mL of sterilized PBS solution, and vibrate ultrasonically for 10min.

[0056] PBS solution (Sangon Biology) formula: NaCl 136.89mM; KCl 2.67mM; NaCl 2 HPO 4 8.1mM; KH 2 PO 4 1.76mM; 121°C, 15min, sterilized.

[0057] 3) Take out the root of Salvia miltiorrhiza, wash it with sterile water (50...

Embodiment 2

[0065] Embodiment 2: the acquisition of the aseptic vaccine of Salvia miltiorrhiza

[0066] The aseptic vaccine of Salvia miltiorrhiza used was obtained through the following steps:

[0067] 1) Select plump seeds of Salvia miltiorrhiza, rinse with running water, treat with 75% alcohol, rinse with sterile water, treat with 5% NaClO solution, rinse with sterile water, absorb excess water with sterile filter paper, and place in a tissue culture room at 28°C Germination culture, the medium is MS medium (pH 5.8-6.0), the culture conditions are temperature 25-28°C, light intensity 2800-3000lx, and light time 14h / d.

Embodiment 3

[0069] Embodiment 3: binary co-cultivation of endophytic fungi and sterile vaccines of Salvia miltiorrhiza

[0070] 1) Get the Mucor circinelloides (M.circinelloides) DF20 gained in Example 1, under aseptic conditions, pick the mycelium of an inoculation loop, inoculate it in 100ml sterile PDA liquid medium, place it at 28°C, 150rpm shaker culture for 3-5 days, take an appropriate amount of bacterial solution and dilute it with sterile PDA medium to form a bacterial suspension (10 6 ~10 7 CFU / mL).

[0071] 2) Take the aseptic seedlings of 3-4 leaf age Salvia miltiorrhiza obtained in Example 2, and transplant them into the aseptic culture medium (peat: vermiculite: perlite=3:1:1) equipped with 100ml MS medium, Cultivate in a tissue culture room at 25°C for 10-15 days, take 1ml of the DF20 bacterial suspension obtained in step 1) and inoculate the roots of Salvia miltiorrhiza seedlings, and inoculate 1ml of sterile PDA liquid culture medium grown on a shaker under the same con...

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Abstract

The invention discloses a mucor circinelloides DF20, and the preservation number of the mucor circinelloides DF20 is CGMCC NO. 20742. The invention also discloses an application of the mucor circinelloides DF20, and the mucor circinelloides DF20 can be used for promoting the root development of salvia miltiorrhiza and the synthesis of tanshinone components. The strain DF20 an be used for effectively promoting the root system development of salvia miltiorrhiza and effectively promoting the biosynthesis and accumulation of tanshinone components through co-culture with salvia miltiorrhiza plants,and has a relatively high application value.

Description

technical field [0001] The invention relates to an endophytic fungus of the root system of Danshen and its application for promoting the development of the root system of Danshen and the synthesis of tanshinone components. Background technique [0002] Salvia miltiorrhiza Bunge. is a plant of the genus Salvia in the Labiatae family. Its dried root or stem root is often used as medicine. According to its traditional efficacy, Danshen is widely used in the treatment of cardiovascular and cerebrovascular diseases in modern times, and its curative effect is remarkable. The medicinal components of Danshen include water-soluble salvianolic acids and fat-soluble tanshinones. Water-soluble salvianolic acid compounds, mainly salvianolic acid A, B, C, caffeic acid, danshensu, etc., have anti-thrombosis, anti-oxidation, cell protection, anti-coagulation and blood lipid regulation effects. Fat-soluble tanshinone components mainly include tanshinone I, dihydrotanshinone (dihydrotanshin...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G31/00A01G7/06A01N63/30A01P21/00C12R1/785
CPCA01G7/06A01G31/00A01N63/30
Inventor 陈海敏漆瑶徐琳娜杨东风张海花梁宗锁张晓丹
Owner ZHEJIANG SCI-TECH UNIV
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