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Mucor circinelloides strain with high grease yield and application thereof

A technology of Mucor cuspidatum and strain, applied in the field of microorganisms, can solve the problems of low fatty acid accumulation ability, affecting the application prospect of Mucor cuspidatum and the like

Inactive Publication Date: 2015-09-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The low fatty acid accumulation ability of the reported M. circiniferii strains seriously affects the application prospect of M. circiniferii in the production of functional oils and bioenergy

Method used

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  • Mucor circinelloides strain with high grease yield and application thereof
  • Mucor circinelloides strain with high grease yield and application thereof
  • Mucor circinelloides strain with high grease yield and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A. Activation of bacteria

[0072] Under sterile conditions, take out the spore glycerin tube of Mucor circinifolia WJ11 (or CBS277.49) from the ultra-low temperature refrigerator at -80°C, draw 100 μL into a Erlenmeyer flask with baffles containing 150 mL of the following activation medium, and In the shaker sold by Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd. under the trade name of ZHWY-2102 rotary constant temperature shaker, the temperature is 30 ° C and the rotation speed is 150 rpm. .

[0073] B. Seed cultivation

[0074] Under sterile conditions, according to the volume of the activated bacterial seed solution obtained in step A is 10% of the volume of the seed culture medium, the activated bacterial seed liquid is inserted into the Erlenmeyer flask with baffles that 150mL of the seed medium is housed , in the shaker at a temperature of 30° C. and a rotation speed of 150 rpm for 24 hours at a constant temperature to obtain a seed culture sol...

Embodiment 2

[0085] A. Activation of bacteria

[0086] Under sterile conditions, take out the spore glycerol tube of Mucor circinifolia WJ11 (or CBS277.49) from the -80°C ultra-low temperature refrigerator, draw 100 μL into a Erlenmeyer flask with baffles containing 100 mL of the following activation medium, and In the shaker sold by Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd. with the trade name ZHWY-2102 rotary constant temperature shaker, the temperature is 30 ° C and the rotation speed is 200 rpm. .

[0087] B. Seed cultivation

[0088] Under sterile conditions, according to the volume of the activated bacterial seed solution obtained in step A is 10% of the volume of the seed culture medium, the activated bacterial seed liquid is inserted into the Erlenmeyer flask with baffles that 150mL of the seed medium is housed , and cultivated at a constant temperature for 16 hours under the conditions of a temperature of 30° C. and a rotation speed of 150 rpm in the shaker...

Embodiment 3

[0099] A. Activation of bacteria

[0100] Under sterile conditions, take out the spore glycerol tube of Mucor circinifolia WJ11 (or CBS277.49) from the -80°C ultra-low temperature refrigerator, draw 100 μL into a Erlenmeyer flask with baffles containing 100 mL of the following activation medium, and In the shaker sold by Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd. with the trade name ZHWY-2102 rotary constant temperature shaker, the temperature is 30 ° C and the rotation speed is 200 rpm. .

[0101] B. Seed cultivation

[0102] Under sterile conditions, according to the volume of the activated bacterial seed solution obtained in step A is 5% of the volume of the seed culture medium, the activated bacterial seed liquid is inserted into the Erlenmeyer flask with baffles that 150mL of the seed medium is housed , in the shaker at a temperature of 30° C. and a rotation speed of 150 rpm for 24 hours at a constant temperature to obtain a seed culture solution. ...

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Abstract

The invention relates to a Mucor circinelloides strain with high grease yield and application thereof. The invention discloses a Mucor circinelloides strain WJ11 and application thereof (a method for producing grease by using the strain). The method comprises the following steps: activating the strain to obtain an activated strain solution, carrying out seed culture under sterile conditions to obtain a seed culture solution, carrying out fermentation culture to obtain a fermentation culture solution, filtering, carrying out freeze-drying to constant weight to obtain a grease-containing Mucor circinelloides WJ11 dry strain, grinding, and extracting grease by a chloroform-methanol process. The Mucor circinelloides strain WJ11 has the advantages of high growth rate and high fatty acid content (up to 30-40% of biomass); the fatty acid content is 2-3 times of the control strain; and the biochemical analysis result shows that the Mucor circinelloides strain WJ11 has obviously higher enzyme activity related to fatty acid accumulation than the control group. The Mucor circinelloides strain has very wide application prospects when being used in microbial grease-producing industry and microbial grease accumulation analysis.

Description

【Technical field】 [0001] The invention belongs to the technical field of microbes. More specifically, the invention relates to a strain of Mucor circinifolia and its application, in particular to a strain of Mucor circinzitum capable of high-yielding fatty acid and its fermentation and cultivation method for producing oil. 【Background technique】 [0002] Microbial oil, also known as single-cell oil, refers to the oil accumulated by microorganisms under certain conditions (such as a certain nutrient limitation, such as nitrogen source limitation). Microorganisms with oil content greater than 20% of biomass are called oleaginous microorganisms. [0003] The earliest research on the use of microorganisms to produce oil can be traced back to World War I, when Germany planned to use certain strains of Endomyces and single-celled algae Fusarium to produce oil to solve the shortage of edible oil question. Then the United States, Japan and other countries also began to study the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/64C12R1/785
CPCC12P7/6409C12N1/145C12R2001/785
Inventor 宋元达唐鑫张怀渊
Owner JIANGNAN UNIV
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