Preparation method of mixed microbial agent and application of same in high temperature composting

A technology of mixing bacterial agents and microorganisms, which is applied in the field of environmental microorganisms, can solve the problems of limited diffusion speed and increased composting costs, and achieve the effects of long storage period, shortened composting treatment time, and accelerated diffusion

Active Publication Date: 2018-12-18
福建省致青生态环保有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these bacterial agents are mainly composed of bacteria. Although their proliferation speed is fast, their diffusion speed is limited, especially in organic wastes with relatively large looseness such as straw and cow dung.
Although artificial turning can promote the spread of strains in the heap, it will inevitably increase the cost of composting

Method used

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  • Preparation method of mixed microbial agent and application of same in high temperature composting
  • Preparation method of mixed microbial agent and application of same in high temperature composting
  • Preparation method of mixed microbial agent and application of same in high temperature composting

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Embodiment 1

[0016] Example 1: Preparation of compound bacterial agent and its application in composting.

[0017] 1. The culture medium involved in the embodiment:

[0018] The composition of PDA medium is: potato (200 g / L) (with leaching juice), glucose (20 g / L), and 20 g of agar powder is added to it to make solid PDA medium; the composition of LB medium is: tryptone (10 g / L), yeast extract (5 g / L), NaCl (10 g / L), pH adjusted to 7.0, and 20 g agar powder can also be added to it to make solid LB medium; CPDA medium The composition is: potato (200 g / L) (with leaching juice), glucose (1.25 g / L), potassium dihydrogen phosphate (2 g / L), magnesium sulfate (0.5 g / L), water 1000 mL, pH 6.0.

[0019] 2. Strain activation: Inoculate Mucor circiniferatum on solid PDA medium, culture at a constant temperature of 28°C for 6 days, wash the spores in the plate with 10 mL sterilized distilled water into a 50 mL sterilized Erlenmeyer flask, and use a hemocytometer Count and adjust the spore concentra...

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Abstract

The invention provides a preparation method of a mixed microbial agent, the mixed microbial agent, and an application of same, and belongs to the technical field of environmental microorganisms. The preparation method includes: activating Mucor circinelloides, Bacillus tequilensis and Bacillus velezensis, mixing the spores of the Mucor circinelloides with the other two bacteria according to ratioof 1:3:3, and inoculating the mixed bacteria on a CPDA culture medium, culturing the mixed bacteria at 28 DEG C for 48 h in a constant temperature shaking table at 150 rpm to obtain a mixed microbialliquid. In the invention, by means of the character that the Mucor circinelloides is high in growth speed and can carry and diffuse bacteria, the Mucor circinelloides, applied to the aerobic composting of organic wastes, can enhance the diffusion of organic waste degrading bacteria in a heap, so that composting period is shortened and composting efficiency is increased effectively. Compared with simple microbial agent, the mixed microbial agent has great adaptability and stable performance, and has an extensive application prospect of quick composting reutilization of the organic wastes.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a compound bacterial agent used in aerobic composting and its preparation method and application. Background technique [0002] Composting is also called aerobic composting treatment. Under artificial control conditions, relying on bacteria, actinomycetes, fungi and other microorganisms widely distributed in nature, artificially promotes the microbiological process of transforming biodegradable organic matter into stable humus. It is currently the most commonly used effective method for harmless and resourceful treatment of organic waste. Organic waste (such as straw, kitchen waste, livestock and poultry manure, etc.) usually contains rich N, P and a certain amount of trace elements. After aerobic composting, the organic matter is decomposed and transformed into humus, which is more conducive to plant absorption and utilization. The most suitable fertilizer ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F15/00C05F17/00C05F11/08C12R1/07C12R1/785
CPCC12N1/14C12N1/20C05F3/00C05F11/08C05F17/00C05F11/00Y02W30/40
Inventor 石振华张琪廖斌斌杨祖沐许清霖游成本吴文亮陈开财吴志伟张望文黄盛发
Owner 福建省致青生态环保有限公司
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