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Method for producing microbial oil by using bacteria and enzymes to jointly ferment corn straw

A technology of microbial oil and fermented corn stalks, which is applied in the field of microbial fermentation to reduce environmental pollution and reduce production costs

Active Publication Date: 2020-07-14
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the oleaginous bacteria that are widely studied and used include Rhodosporidium toruloides, Cryptococcus, Trichoderma koningeni and Cellulomonas chrysogenum, and there is no joint production of corn straw by Mucor circinifolia and Yarrowia lipolytica. Report on microbial oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] Preparation of strains:

[0016] The strain Mucor circinelloides WJ11 preserved in our laboratory (Complete genome sequence of a high lipid-producing strain of Mucor circinelloides WJ11 and comparative genome analysis with a low lipid-producing strain CBS 277.49, PloS One, 2015, 10(9):e0137543) and Yarrowia lipolytica CICC1778 (Metabolic flux analysis of lipid biosynthesis in the yeast Yarrowia lipolytica using 13C-labled glucose and gaschromatography-mass spectrometry, Plos One, 2016, 11(7): e0159187).

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Abstract

The invention discloses a method for producing microbial oil by using bacteria and enzymes to jointly ferment corn straw, and belongs to the field of microbial fermentation. The method utilizes the mutual synergistic effects of mucor circinelloides, yarrowia lipolytica and cellulase to ferment corn straw; and fermentation conditions are as follows: the adding amount of the cellulase is 1.5%; the strain ratio of the mucor circinelloides to the yarrowia lipolytica is 1: 1.5; total inoculation amount is 10%; the rotating speed of stirring is 200 rpm / min; fermentation temperature is 31 DEG C, andfermentation time is 54 h; and the fermentation broth of the corn straw per liter can obtain 1.24 g of microbial oil. The method can directly convert lignocellulose, so that the synchronization of cellulose-monosaccharide-oil can be realized in one step, therefore, production costs can be greatly reduced, and pollution to environment can be reduced.

Description

technical field [0001] The invention relates to a method for producing microbial oil by bacterial enzyme co-fermentation of corn stalks, which belongs to the field of microbial fermentation. The fermenting bacteria enzyme of the present invention relates to Mucor circinosa, Yarrowia lipolytica and cellulase. Background technique [0002] With the increasing shortage of fossil resources, it is the general trend to develop renewable oil resources through multiple channels. Oleaginous microorganisms can accumulate a large amount of triglycerides in cells, and it is inevitable for sustainable development to produce oil through microbial transformation of renewable resources and partially replace fossil resources. In addition, a small number of fungi, microalgae, bacteria, etc. can also synthesize functional polyunsaturated fatty acids that are very beneficial to human health, such as arachidonic acid (Arachidonic acid), γ-linolenic acid (γ-linolenic acid), docosan Docosahexaen...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/14C12P7/64C12N1/16C12N1/14C12R1/785C12R1/645
CPCC12P39/00C12P19/14C12P7/6463C12N1/16C12N1/14C12P2201/00Y02E50/10
Inventor 张瑶宋元达王艳霞
Owner SHANDONG UNIV OF TECH
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