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Rhodotorula mucilaginosa for degrading penicillin as well as application thereof

A technology of Rhodotorula marcescens and penicillin, applied in the field of microorganisms, can solve the problems of not much, low concentration of penicillin, low efficiency of penicillin degradation, etc., and achieve high degradation effect

Active Publication Date: 2018-09-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, although there are examples of screening bacterial strains capable of degrading penicillin, there are not many studies at home and abroad, and there are problems such as low penicillin concentration tolerance and low penicillin degradation efficiency, which cannot meet the requirements of bacterial residue treatment in actual production. needs

Method used

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  • Rhodotorula mucilaginosa for degrading penicillin as well as application thereof
  • Rhodotorula mucilaginosa for degrading penicillin as well as application thereof
  • Rhodotorula mucilaginosa for degrading penicillin as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: strain screening

[0027] Weigh each 10g of the sample soil, put them into conical flasks equipped with 100ml of aseptic and one layer of glass beads respectively, vibrate on a shaker for 10min, fully break up, and make 10 -1 g·L -1 Bacterial suspension, diluted 10 times with distilled water to 10 -5 g·L -1 , 10 -6 g·L -1 , 10 -7 g·L -1 Concentration, these three concentrations of bacterial suspension were respectively applied to the pre-prepared YPD medium containing penicillin concentration of 12g / L, and the amount of application was 100 μL. Cultured in a constant temperature incubator at 30°C. After culturing for 24 hours, the grown strain was transferred to the YPD medium with a concentration of 20 g / L penicillin, and after several separation and purification operations, a single colony strain was obtained, which was named Anti-Pen20.

[0028] Such as figure 1 As shown, under the transmission electron microscope, the cells are oval, without fl...

Embodiment 2

[0029] Embodiment 2: Physiological and biochemical studies of bacterial strains

[0030] (1) Optimum growth temperature experiment

[0031] The strains were inoculated on YPD medium and cultured in constant temperature incubators at 25°C, 30°C, 37°C, and 40°C for 1-2 days, and the growth of each strain was observed and recorded. It is known that the optimum growth temperature range of the strain is 25-30°C.

[0032] (2) Optimum growth pH experiment

[0033] Prepare YPD medium with pH values ​​of 3.0, 4.0, 5.0, 6.0, 7.0, and 8.0, adjust the pH value with HCl solution and NaOH solution, and measure the pH value with a pH meter. The strains were inoculated on starch medium with different pH values, cultured in a constant temperature incubator at 30°C for 1-2 days, and the growth of each strain was observed and recorded. It is known that the optimum growth pH range of the strain is 4.0-7.0.

[0034] (3) The growth of the strain

[0035] Strains under test medium conditions: K...

Embodiment 3

[0046] Embodiment 3: detection of penicillin concentration in fermented liquid

[0047] Set up 3 groups of detection medium, the first group added penicillin to the detection medium so that the concentration was 20g / L and did not add bacterial solution, the second group added penicillin to the detection medium to make the concentration 20g / L and inoculated 10 % Anti-Pen20 bacteria solution, the third group inoculated 10% Anti-Pen20 bacteria solution directly in the detection medium without adding penicillin, placed it in a constant temperature shaker at 30°C, and then took the fermentation broth for treatment, and passed the high-performance liquid phase The chromatograph analyzes the fermented liquid, taking the penicillin standard solution (0.5g / L, 1g / L, 2g / L, 5g / L penicillin solution, using the detection medium as solvent) as the standard, the results are as follows image 3 As shown, the results show that the concentration of penicillin in the first group is almost unchang...

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Abstract

The invention discloses rhodotorula mucilaginosa for degrading penicillin as well as application thereof, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa was preserved in China Center for Type Culture Collection (CCTCC) on April 16, 2018, the preservation number is CCTCC M2018202, and the preservation address is Wuhan University, Wuhan, China. Compared with the existing bacterial strain with penicillin degrading capability, the rhodotorula mucilaginosa has the following advantages: the penicillin tolerance concentration (20 g / L) is 50 times that of the knownother bacterial strains in China; the penicillin concentration reducing value within the same time is far higher than the value which other bacterial strains can reach to; the rhodotorula mucilaginosais expected to be applied to treatment on bacterial residues in the actual production; and the capability of degrading the remained penicillin in the bacterial residues is extremely high.

Description

technical field [0001] The invention relates to a rhodotorula marcescens capable of degrading penicillin and an application thereof, which belongs to the technical field of microorganisms. Background technique [0002] Since penicillin was discovered in 1928, it has been widely used in clinical medicine because of its antibacterial properties, and it is still widely used today. Penicillin can hinder the synthesis of bacterial mucin, so that the cell wall cannot be synthesized smoothly, which in turn leads to the rupture and death of bacteria. During the fermentation process of some penicillin pharmaceutical factories, a large amount of solid waste residues will be produced, mainly containing microbial mycelia, organic matter that has not been metabolized, inorganic salts, a small amount of residual penicillin and its degradation products, and penicillin residues can be produced Organic fertilizers are used to increase the production of vegetables and grain crops, and are us...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23L5/20C05F11/00C05F17/00C12R1/645
CPCA23V2002/00A23L5/28C05F11/00C12N1/16C05F17/20C12N1/145C12R2001/645A23V2250/76Y02W30/40
Inventor 徐建中王礼君张伟国冯丽妍
Owner JIANGNAN UNIV
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