A nitrogen-fixing bacteria pj12 that can degrade petroleum and tolerate petroleum and its application

Abstract

The invention relates to the field of microorganisms, and specifically discloses a nitrogen-fixing bacterium PJ 12 that can degrade petroleum and tolerate petroleum, and its preservation number is: CGMCC No.14659. The nitrogenase activity analysis shows that the bacterial strain provided by the invention has strong nitrogenase activity, and all have relatively high nitrogenase activity under the condition of 5-21% oxygen concentration. At 1%-5% petroleum concentration, it can still maintain its nitrogenase activity. Not only that, the bacterial strain provided by the present invention also has the ability to degrade long-chain alkanes, and the degradation ability can reach 37-100%. In future oil-contaminated soil remediation, the bacterial strain provided by the present invention can balance nitrogen, It has a good and positive application prospect in degrading petroleum pollutants.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a nitrogen-fixing bacterium strain capable of degrading petroleum and tolerant to petroleum. Background technique [0002] In bioremediation, microorganisms play an important role. In the process of natural evolution, microorganisms metabolize organic substances through different catalytic mechanisms, and "store" functional enzymes as raw materials for evolution, which can coexist with various types of organic components, and are particularly good at using natural or synthetic organic substrates as carbon Since the 1940s, the use of microorganisms to control oil pollution has been considered a powerful strategy. [0003] During the biodegradation process, the addition of nutrients plays an important role in activating indigenous microorganisms, promoting microbial metabolism and degrading petroleum hydrocarbons in the environment. However, excessive use of nutrients can lead to some pot...

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Problems solved by technology

However, excessive use of nutrients can lead to some potential problems, such as excessive consumption of oxygen in the environment, forming an anaerobic environment, which in turn reduces the ability of indigenous microorganisms to degrade oil

Aerobic biodegradation of petroleum is often limited by combined nitrogen levels

In 2011, Mercer summarized the reported effects of biological treatment (bio-stimulation and degradative agent addition) in beach pollution treatment, and found that 10 reports were successful and 6 reports failed

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