40results about How to "Increase the number of nodules" patented technology

A common culturing-fermenting method of rhizobia and P-decomposing bacillus megaterium for preparing multi-function bacterial fertilizer includes such steps as inoculating rhizobia in the fermentive culture medium, culturing in constant-temp gyratory shaker for 24-36 hr, inoculating said bacillus megaterium in it, and culturing in constant-temp gyratory shaker for 48-60 hr.

The invention discloses novel application of florigen gene GmFT5a in promoting biological nitrogen fixation for leguminous plants and improving nitrogen and phosphorus nutrient efficiency. The florigen gene GmFT5a starts expressing upon rapid development of root nodules and locating at nuclei and cytoplasm of cortical cells of the root nodules, thereby significantly increasing the quantity and weight of soybean root nodules and dinitrogenase activity under both long and short durations of sunshine, increasing nitrogen and phosphorus contents in plants, and finally increasing biomass quantity of transgenic plants. Nitrogen and phosphorus nutrition in leguminous crops can be efficiently utilized; candidate gene resources are provided for high-yield molecular breeding with good photoperiod eurytopicity; the florigen gene GmFT5a has significant theoretical and practical significances in the development of environment-friendly sustainable ecological agriculture.

The invention discloses a humic acid fertilizer synergist with an ammonia volatilization inhibition effect and a preparation method thereof. The synergist is prepared from the following raw materials in parts by weight: 65-75 parts of humic acid soil, 3-6 parts of a strain, 12-14 parts of a carbon source, 6-8 parts of a nitrogen source, 35-45 parts of water, 2-3 parts of medium trace elements, 0.08-0.12 part of ferrous carbonate, 0.6-0.8 part of a film forming agent and 1.5-3 parts of an organic acid regulator. According to the synergist prepared by utilizing a humic acid synergetic solution, all components in the humic acid synergetic solution are purely natural substances, have excellent environmental friendliness, can be completely decomposed, and can be used for promoting absorption of nutrition in soil, increasing the yield of crops, improving the quality of agricultural products, reducing nutrition loss, avoiding secondary pollution, reducing agricultural production cost and supplying trace elements in the soil. The humic acid fertilizer synergist has the characteristics of wide raw material source, low cost, simple production process, convenience in use in fertilizer production and the like, and has a wide application prospect in synergizing production.

The invention relates to the field of biological processing, and in particular relates to a processing method of kitchen wastes and the application of a processed product as an organic fertilizer. The processing method is simple, and the kitchen wastes are effectively utilized. A kitchen waste processed product is rich in nutrients and can be used for preparing the organic fertilizer. Moreover, the invention also provides the organic fertilizer. The organic fertilizer comprises the kitchen waste processed product and acceptable elements in the fertilizer. By applying the organic fertilizer containing the kitchen waste processed product, the number of root nodules of summer soybeans can be effectively increased, the chlorophyll content, leaf nitrogen content, net photosynthetic rate and yield of the summer soybeans are improved, and increase of the farmer income is promoted.

The invention relates to a low-temperature phosphorus-dissolving Sinorhizobium sp. and its bacterial agent. The strain name is ATHC‑2, and the classification name is Sinorhizobium sp.. The depository unit is the General Microbiology Center of China Microbiological Culture Preservation Management Committee. The number is CGMCC No.10651, the preservation date is: March 20, 2015, and the preservation address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The bacterial strain ATHC-2 of the present invention can withstand the influence of low temperature on its growth, and has strong phosphorus-dissolving activity. The impact of low temperature stress on soybean nodulation and nitrogen fixation in agricultural production can be alleviated by applying low temperature resistant rhizobia agents, improving soil fertility, reducing the use of chemical fertilizers, and improving the yield and quality of soybeans and other crops.

The invention discloses a transgenic plant breeding method for increasing the number of root nodules and promoting the development of root nodules, overexpressing the gene shown in SEQ ID NO: 1 or SEQ ID NO: 2 in the sequence list in the plant or its homologous / equivalent Genes to obtain transgenic plants with increased number of nodules and / or enhanced nodule development compared to normal plants. Using the method of the invention to construct transgenic plants can significantly increase the number of root nodules and promote the maturity of root nodules, and has great practical value in theoretical research and agricultural and forestry applications.

The invention discloses GmMDH12 Application of genes in promoting soybean nodulation and nitrogen fixation. malate dehydrogenase gene GmMDH12 Control of malate synthesis in root nodules and its use in promoting root nodulation in leguminous crops. The present invention finds that, GmMDH12 Has the function of regulating malic acid synthesis in soybean nodules, overexpression GmMDH12 Significantly increased the concentration of endogenous malic acid in root nodules of soybean transgenic compound plants, increased the number of nodules and the biomass of nodules, and thus increased the nitrogen content and biomass of plants. According to the present invention, GmMDH12 Genes have the potential to improve the biological nitrogen fixation function of legume crops through transgenic technology, which has important theoretical and practical significance for improving the efficient use of crop nutrients and the development of environmentally friendly and sustainable agriculture.

The invention discloses a method for promoting the growth of clover and / or increasing the yield of clover and the bacterial agent used therein. The method for promoting the growth of clover and / or increasing the yield of clover comprises the step of mixing the seeds of clover with the inoculum before sowing the soil; the inoculation contains the metabolites of Rhizobium clover or / and the Rhizobium clover ; The strain number of the Rhizobium clover bacteria is 92-29, and its preservation number in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.15533. Clover rhizobia 92-29 of the present invention significantly promotes the growth of clover and significantly improves the output of clover under acidic soil conditions: improve the plant height, nodulation rate, root nodule number and / or root nodule weight of clover; increase the plant fresh weight and dry weight of clover Heavy, increases clover's fresh grass yield and dry grass yield. The invention has broad application prospects in the clover planting industry.