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Method for promoting the growth of clover and/or increasing the yield of clover and inoculum used therefor

A technology of clover and yield, applied in the field of agricultural microorganisms, can solve the problems of soil nitrogen consumption and soil fertility decline, and achieve the effects of increasing plant fresh weight and dry weight, increasing hay yield, and increasing fresh grass yield

Active Publication Date: 2021-12-10
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the planted legumes do not have corresponding high-efficiency rhizobia strains to symbiotically form nodules and fix nitrogen in the air, they will completely depend on the combined nitrogen in the soil, and instead of supplementing it, they will consume nitrogen in the soil, resulting in soil Decreased fertility

Method used

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  • Method for promoting the growth of clover and/or increasing the yield of clover and inoculum used therefor
  • Method for promoting the growth of clover and/or increasing the yield of clover and inoculum used therefor
  • Method for promoting the growth of clover and/or increasing the yield of clover and inoculum used therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1, Isolation and Identification of Rhizobium trifolii (Rhizobium trifolii) 92-29CGMCC No.15533

[0039] 1. Isolation of strains

[0040]Collect wild white clover root nodules from Qujing area, Yunnan Province, use a flat plate (get mannitol 5g, glycerol 5g, yeast extract powder 1g, dipotassium hydrogen phosphate 0.5g, calcium sulfate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.1g , the mass content is 1% ammonium molybdate aqueous solution 1ml and the mass content is 1% boric acid aqueous solution 1ml, 0.5% Congo red 1ml, 20g agar, distilled water 1L, pH value is 6.8-7.0) streak separation obtains bacterial strain 92-29 .

[0041] 2. Identification of strains

[0042] 2.1. Morphological identification

[0043] The strain 92-29, which is in the logarithmic growth phase and the colony size is stable, is isolated and purified in the above step 1, and the single colony state is observed, mainly including the size, color, transparency, humidity, surface state ...

Embodiment 2

[0050] Embodiment 2, the preparation of Rhizobium clover 92-29 inoculum

[0051] 1. Incline culture of Rhizobium clover 92-29

[0052] The Rhizobium cloverii 92-29 of Example 1 was picked and inoculated on a solid medium for slant culture, and cultured at 28° C. for 56 hours to obtain the Rhizobia cloverii 92-29 cultured on a slant.

[0053] 2. Activation of bacteria

[0054] Pick the Rhizobia cloverii 92-29 cultured on the slant in step 1, inoculate it in 200 mL of liquid medium, and culture it with shaking at 28° C. and 150 rpm for 48 hours to obtain the Rhizobia cloverii 92-29 bacterium liquid.

[0055] 3. Seed tank cultivation

[0056] Get 3L of the Rhizobium clover 92-29 bacterium liquid of step 2, insert it into a 100L seed tank (containing 60L liquid medium) and carry out seed culture, and cultivate it with aeration and stirring at 28°C and 150rpm for 48 hours to obtain the Rhizobium clover 92 -29 seed liquid.

[0057] 4. Fermentation tank culture

[0058] Accordin...

Embodiment 3

[0062] Embodiment 3, the growth situation comparison (tolerance test) of Rhizobium clover 92-29 at different pHs on the acid medium

[0063] After the above liquid medium is sterilized, use sterile 1% NaOH solution and 1% HCl solution to adjust the pH of the medium to 4.2, 4.4, 4.5, 4.6, 4.8, 5.0, 5.5, 6.0, 7.0 to obtain different pH values The liquid medium of each treatment was repeated three times. The strains of Rhizobium cloverii 92-29 and R. trifolii ACCC 18019 were respectively inoculated in liquid medium with different pH values. After 48 hours of shaking culture at 150rpm and 28°C, , using the liquid culture medium without inoculation of each treatment as a blank control to determine its OD 600 value. The results are shown in Table 1, showing that Rhizobium clover 92-29 can grow in the range of pH 4.2-7.0, Rhizobium clover ACCC 18019 can only grow in the range of pH 4.8-7.0, and can grow in the pH range of 4.8-7.0. In the range of 4.2-7.0, the growth status of Rhizo...

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Abstract

The invention discloses a method for promoting the growth of clover and / or increasing the yield of clover and the bacterial agent used therein. The method for promoting the growth of clover and / or increasing the yield of clover comprises the step of mixing the seeds of clover with the inoculum before sowing the soil; the inoculation contains the metabolites of Rhizobium clover or / and the Rhizobium clover ; The strain number of the Rhizobium clover bacteria is 92-29, and its preservation number in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.15533. Clover rhizobia 92-29 of the present invention significantly promotes the growth of clover and significantly improves the output of clover under acidic soil conditions: improve the plant height, nodulation rate, root nodule number and / or root nodule weight of clover; increase the plant fresh weight and dry weight of clover Heavy, increases clover's fresh grass yield and dry grass yield. The invention has broad application prospects in the clover planting industry.

Description

technical field [0001] The invention relates to a method for promoting the growth of clover and / or increasing the yield of clover in the field of agricultural microorganisms and the bacterial agent used therein. Background technique [0002] Trifolium is the most widely distributed genus in the legume family, and it is a short-lived perennial leguminous herb. There are more than 360 species of clover all over the world, of which about 25 species have high agricultural economic value. my country is a country with large grasslands, and clover is widely planted in our country, mainly distributed in temperate to tropical regions. There are wild species and cultivated species as far as Yunnan in the south and Heilongjiang in the north. Red clover, white clover and clover are more cultivated in my country Miscellaneous clover. Clover is an excellent forage grass with nitrogen-fixing ability. It is rich in protein and minerals. It has strong cold resistance, a long green period, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/41
CPCA01N63/00C12N1/20A01N63/10
Inventor 马晓彤刘慧琴顾金刚李世贵张瑞福
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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