36results about How to "Nodulation rate is high" patented technology

The invention relates to Bradyrhizobium sp. LX‑JX01 and its application. The preparation method of the bradyrhizobium LX-JX01 bacterial agent of the present invention comprises the steps of liquid DM medium preparation, solid DM medium preparation, strain activation and rhizobia culture. The strain can nodulate symbiosis with Dalbergia balata. When Dalbergia japonica LX-JX01 bacterial agent of the present invention was used to plant Dalbergia japonica seedlings, its nodule nodule number increased by 3823% compared with the control, the fresh weight of nodules increased by 1038%, the dry weight of the aboveground part of the plant increased by 47.22%, and the seedling height and plant diameter are increased by 1030% and 42.3% respectively, therefore, the bacterial agent of the present invention can promote the growth of Dalbergia balsamicum. Therefore, the fungal agent of the present invention has broad application prospect in the plantation of Dalbergia balsamanii artificial forest.

The invention relates to mesorhizobium KDRM295 which is separated from acacia root nodule, contains ACC deaminase, and can efficiently nodulate and promote growth of the acacia, and an application thereof. The mesorhizobium KDRM295 is now preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCCNO: M2012331. The mesorhizobium KDRM295 contains ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, reduce the level of plant cell for synthesizing ethylene, lighten the inhibition effect of ethylene to infection of the root nodule, improve the nodulation rate of root nodule and acacia and the level of symbiotic nitrogen fixation, provide high-level nitrogen for the growth of the acacia on the barren wasteland without fertilization, promote the growth of acacia seeding, and increase mature biomass for the acacia, thereby realizing high yield of the acacia mature with low inoculation investment, and playing a role of raising seeding and afforestation for acacia.

The invention relates to a mesorhizobium KDRM185 capable of being separated from an acacia root nodule, containing ACC deaminase, efficiently nodulating and promoting acacia growth and application thereof. A mesorhizobium KDRM185 strain is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC No: M2012329. The mesorhizobium KDRM185 disclosed by the invention contains the ACC deaminase and can decompose the ACC into alpha-ketobutyrate and NH3, lower the level of plant cells on synthesizing ethylene, reduce the inhibiting effect of the ethylene on rhizobium infestation, enhance the nodulation rate and symbiotic nitrogen fixation level of rhizobia and acacia, provide high-level nitrogen for the growth of the acacia on unfertilized sterile wastelands, promote the growth of acacia seedlings and increase the biomass of acacia lumbers, thereby achieving the high yield of the acacia lumbers through low-cost inoculation investment and playing a role in acacia seedling culture and forestation.

The invention discloses a rhizobium strain WYS3R1 and an application thereof, belonging to the technical field of microorganisms. The strain was isolated and purified from fresh nodules of the genus Cassia forage, detected by PCR nodA gene, and identified by 16S rDNA molecular biology, identified as Bradyrhizobium ( Bradyrhizobium ) of the new strain and named Wuyuan 1. It was deposited in the China Center for Type Culture Collection on April 10, 2018, and the deposit number is CCTCC NO: M2018190. The rhizobium WYS3R1 of the present invention has the characteristics of efficient nodulation and strong nitrogen fixation ability through the laboratory pot plant back-connection test, and inoculation of the rhizobium can significantly increase the number of nodules, the nitrogen-fixing efficiency of nodules, the biomass and plant nitrogen of the forage of the genus Cassia content, and then achieve the effect of fertilizing soil fertility and improving the ecological environment.

The invention relates to mesorhizobium KDRM319 and application thereof. The mesorhizobium KDRM319 is separated from acacia root nodule, contains ACC deaminase and can efficiently nodulate and promote acacia growth. A mesorhizobium KDRM319 strain is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC NO.M2012332. The mesorhizobium KDRM319 disclosed by the invention contains the ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, lowers the level of synthesizing ethylene from plant cells, reduces the inhibiting effect of ethylene on rhizobium infestation, enhances the nodulation rate and symbiotic nitrogen fixation level of rhizobia and acacia, provides the high-level nitrogen for the growth of the acacia on unfertilized sterile wastelands, promotes the growth of acacia seedlings and increases the biomass of acacia lumbers, thereby achieving the high yield of the acacia lumbers through low-cost inoculation investment and playing a role in the aspect of acacia seedling culture and forestation.