The invention discloses a bradyrhizobium
liquid culture medium and preparation and culture methods thereof. The bradyrhizobium
liquid culture medium is prepared from
fulvic acid,
mannitol, a
yeast extract, K2HPO4, KH2PO4, MgSO4, NaCl, CaCl2, FeCl3, H3BO3, Na2MnO4 and
biotin. The preparation method includes the step that the raw materials and water are mixed and prepared into a solution. The raw materials of the prepared culture medium for culturing bradyrhizobium have wide sources; the preparation method is easy to implement,
fermentation only needs to be conducted once, and no materials needto be added halfway; on this basis, the effect of adopting the prepared culture medium to culture bradyrhizobium is better than that of adopting an ordinary culture medium for culturing, and the culture method includes the steps of activating strains, culturing seeds and conducting
fermentation culture. The
mycelium density of bradyrhizobium cultured by the culture medium in the same period of time is greater; on the premise of meeting a certain quantity of mycelia, the
fermentation time can be shortened; therefore, the effect of the prepared culture medium on the bradyrhizobium growth efficiency is obviously improved.