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Rhizobium and application thereof

A technology of rhizobia and microbial strains, applied in the direction of application, bacteria, fertilizer mixture, etc., can solve the problems of poor nodulation ability and nitrogenase activity, and achieve the effect of improving soybean nitrogen nutrition

Inactive Publication Date: 2014-07-02
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a strain of rhizobia and its application in order to solve the problem that existing soybean-inoculated rhizobia have poor competitive nodulation ability and nitrogenase activity under long-term continuous cropping conditions.

Method used

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  • Rhizobium and application thereof
  • Rhizobium and application thereof
  • Rhizobium and application thereof

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specific Embodiment approach 1

[0012] Specific embodiment 1: In this embodiment, a strain of rhizobia is Bradyrhizobium hailunense S-S20131G64, which has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and the preservation address is Beichenxi, Chaoyang District, Beijing No. 3, Courtyard No. 1, the preservation date is January 9, 2014, and the preservation number is CGMCC No.8693.

[0013] Bradyrhizobium hailunense S-S20131G64 of this embodiment is applied to soil for long-term continuous cropping of soybeans.

[0014] In this embodiment, a strain of rhizobia was isolated and screened from the vigorously growing soybean plants in the long-term soybean continuous cropping experiment site of the Hailun Agricultural Ecological Experiment Station in Heilongjiang. Smooth, the colony diameter reaches 0.8-2.0mm after 5-7 days of culture. Pick a smear of the bacterial lawn and observe it under an oil microscope. The rhizobia are small bacilli of (0.5-0.8)×...

specific Embodiment approach 2

[0016] Specific embodiment two: the application of a strain of rhizobia in this embodiment refers to the application of rhizobia on the soil of long-term continuous soybean cropping.

[0017] The nitrogen-fixing bacteria strain S-S20131G64 of this embodiment is suitable for the long-term soybean continuous cropping soil area in Heilongjiang Province, as one of the conventional soybean cultivation measures.

specific Embodiment approach 3

[0018] Specific embodiment three: the separation of rhizobia S-S20131G64 of the present embodiment is carried out by the following steps: one, choose the vigorous soybean plant of aboveground part from Heilongjiang Hailun Agricultural Ecological Experimental Station long-term soybean continuous cropping experiment, observe the root nodules attached to its roots , pick the pink nodules and place them in a small sampling bottle, and put anhydrous sodium sulfate at the bottom of the bottle as a desiccant. 2. After taking the root nodules back to the laboratory, wash them three times with sterile water, sterilize them with 3% sodium hypochlorite for 5 minutes, and wash the sterilized root nodules with sterile water for 7 times, and keep the last washing water for YMA plate coating Cloth detection root nodule disinfection is complete or not. 3. Use sterile tweezers to transfer a single sterilized and washed tumor to a sterile EP tube, smash the tumor with a sterile gun tip, and the...

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Abstract

The invention discloses rhizobium and application thereof. The invention aims to solve the problem that the conventional rhizobium inoculated by soybean is low in competitive nodulation capacity and nitrogenase activity under long-term continuous cropping conditions. The rhizobium refers to bradyrhizobium hailunense (Bradyrhizobium hailunense) S-S20131G64 and is collected in China General Microbiological Culture Collection Center, the collection address is 3#, courtyard No.1, Beichen west road in Chaoyang District in Beijing, the collection date is 10th, January in 2014, and the collection number is CGMCC No.8693. The application refers to application in long-term soybean continuous cropping soil. The rhizobium has the high-efficiency nodulation characteristic, the soybean biomass can be improved, the nitrogen nutrition of soybeans is improved, and the rhizobium is applied to long-term soybean continuous cropping soil districts in Heilongjiang Province. The invention is applied to the field of agriculture.

Description

technical field [0001] The invention relates to a strain of rhizobia and its application. Background technique [0002] my country is a big producer and consumer of soybeans. Due to the continuous increase of population, the consumption of soybeans is also increasing continuously, and the contradiction between supply and demand is very tense. In order to meet people's demand for soybean products, farmers have to continuously plant soybeans on limited arable land. At present, the continuous cropping area of ​​soybeans has reached more than 35%, and soybeans are typical crops that are not resistant to continuous cropping. Long-term continuous cropping will cause soybean nodules , Nitrogen fixation ability declines, growth and development are hindered, various diseases and insect pests occur year after year, yield and quality decline, or even disappear. At present, most of the rhizobia used for soybean inoculation are isolated from non-long-term continuous cropping conditions,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C12R1/01
Inventor 严君韩晓增冀照君郭爱雪
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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