Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Root nodule azotobacter strain BDYD1 and uses thereof

A slow rhizobium, nitrogen fixation technology, applied in the application, bacteria, biocide and other directions to achieve the effect of improving nitrogen nutrition

Inactive Publication Date: 2010-05-19
SOUTH CHINA AGRI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, although soybeans have a certain planting area in the acidic phosphorus-deficient red soil area, common rhizobia are rarely used in the southern area due to the acidic soil in the south.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Root nodule azotobacter strain BDYD1 and uses thereof
  • Root nodule azotobacter strain BDYD1 and uses thereof
  • Root nodule azotobacter strain BDYD1 and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation and purification of nitrogen-fixing bacteria strain BDYD1

[0038] Take fresh root nodules, rinse them with water, and dry the surface moisture with filter paper. Put it in 95% ethanol for 3 minutes, take it out and wash it with sterile water for 5-6 times, then put it in 0.2% HgCl 2 Sterilize for 5 minutes, take out and rinse with sterile water 5-6 times. Then cut in half on a flame sterilized glass slide, clamp the half tumor with sterile tweezers, mark the incision facing the surface of the YMA (Table 1) medium, incubate at 28°C after inversion.

[0039] After the thalli grow out, select the rhizobia-like colony from the plate and streak on the plate for culture. After 3 days, observe the colony situation, and observe until 15 days, because Bradyrhizobium needs 7 to 15 days to appear colonies. According to the following two aspects, it can be preliminarily judged whether it is rhizobia: ①Colony shape: the colony of rhizobia is round, milky whit...

Embodiment 2

[0042] Example 2 Sequencing of the 16S rDNA sequence of Azotobacter tumefaciens strain BDYD1

[0043] The total DNA of the strain was extracted and subjected to PCR-specific amplification. After the PCR amplification product was checked on 1.0% agarose gel electrophoresis, it was sent to Yingjun Company for sequence determination. The sequencing result is shown in SEQ ID NO:1.

[0044] Eight reference strains were obtained from the GenBank database, and the 16S rDNA partial sequences of the isolated strains and reference strains were analyzed using the software GeneDoc and TREECONW, and a phylogenetic relationship diagram between the isolated strains and the standard strains was constructed, as shown in image 3 shown. Therefore, it was determined that the isolated nitrogen-fixing bacteria strain BDYD1 was a new strain of Bradyrhizobium, and it was named Huagu No.1.

Embodiment 3

[0045] Embodiment 3 tie-back test

[0046] The recertification of the tie-back test adopts the above-mentioned large test tube paper culture device ( Figure 4 ), soybean seed treatment is also as described above.

[0047] Bacterial liquid culture: use an inoculation loop to pick a ring of rhizobia in the test tube and put it into the TY liquid medium. The formula of the TY liquid medium is shown in Table 2. After plugging it with a cotton plug, seal it with kraft paper and put it on the shaker Cultivate until the logarithmic growth phase (about 4 to 6 days), and the culture temperature is 28°C.

[0048] Table 2 TY liquid medium ( / L)

[0049]

[0050] Inoculation treatment: Put the germinated seeds in a petri dish and soak them in the bacterial solution for 15 minutes, plant the seedlings between the filter paper and the tube wall with tweezers, add 1-2mL of the bacterial solution to each seedling to ensure that each seedling The inoculation amount of seedlings reached 1.0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Nodule Azotobacter strain BDYD1 and the application. The strain is obtained by a way that the Rhizobia strains in the soil are respectively captured through different soybeanvarieties; the Rhizobia strains are separated and purified in fresh root nodules; part 16S rDNA of the strain is sequenced; the phylogenetic analysis determines that the strain is a novel Bradyrhizobium strain, which is named as Huagu 1. The Nodule Azotobacter strain BDYD1 of the invention has the characteristics of acid and aluminum tolerance and high level of nodules, which are proved by laboratory pot culture and field experiment. The inoculation of the Rhizobia strain can improve the yield of soybean more than 20 percent to 30 percent. The invention suits for the acid soil area in South China, which can be used as one of the measures for the conventional cultivation of soybean.

Description

technical field [0001] The invention relates to a nitrogen-fixing bacteria strain BDYD1 and its application. Background technique [0002] Biological nitrogen fixation is one of the major basic research topics in life sciences, and it plays an important role in production practice. Symbiotic nitrogen fixation is the most important type of biological nitrogen fixation. The symbiosis between legumes and rhizobia has become the focus of biological nitrogen fixation research because of its strong nitrogen fixation capacity, large nitrogen fixation capacity, and strong stress resistance. A large number of experiments at home and abroad have proved that inoculating rhizobia in most soils can improve the yield and quality of legume crops. Inoculation of high-efficiency rhizobia, as the main measure to increase soybean yield, has been widely used in northern my country. [0003] At present, soybean has become one of the most valuable leguminous crops in the vast acidic phosphorus...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00C12R1/01
Inventor 曹桂芹廖红严小龙
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com