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Iron matrix autotrophic denitrification microbacterium strain and application thereof

A technology of denitrifying bacteria and denitrifying microbacteria, applied in the fields of bioengineering and environmental engineering, can solve problems such as increased operating costs, and achieve the effects of low cost, simple process, and strong practical value

Active Publication Date: 2014-08-13
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the treatment of low C / N sewage, a large amount of additional organic matter needs to be added to meet the process requirements, which will lead to an increase in operating costs

Method used

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  • Iron matrix autotrophic denitrification microbacterium strain and application thereof
  • Iron matrix autotrophic denitrification microbacterium strain and application thereof
  • Iron matrix autotrophic denitrification microbacterium strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Screening and Identification of Bacterial Strains

[0015] (1) Take 30mL of deep sediment from East Lake in Wuhan City, Hubei Province and add it to a closed Erlenmeyer flask containing 100mL of iron-based autotrophic denitrification liquid medium, and then place it on a constant temperature shaker at 30°C with a speed of 150r / min for shaking culture 4~5d, obtain the enriched bacterial liquid;

[0016] (2) Take 30mL of the enriched bacteria solution and add it to the Erlenmeyer flask containing 100mL of iron-based autotrophic denitrification medium, repeat the culture conditions of step (1) for 3 to 5 times, until the nitrate nitrogen in the bacterial suspension has a certain After the removal rate, the domesticated bacteria liquid is obtained;

[0017] (3) Gradual dilution of the domesticated bacteria solution, the dilution gradient is 10 -2 、10 -4 、10 -6 、10 -8 , respectively take 1mL of domesticated bacteria solution and add it to the plate of solid ...

Embodiment 2

[0025] The denitrification characteristics of iron-substrate autotrophic denitrifying Microbacterium W3 were studied through experiments. The method is to inoculate a closed shake flask with 5% inoculum, at pH 6.8~7.0, temperature 30°C, NO 3 - -N initial concentration is 40 mg / L, Fe 2+ The initial concentration of 500mg / L was cultivated for 7 days, during which the NH in the culture medium was continuously monitored. 4 + -N, NO 2 - -N, NO 3 - -N and Fe 2+ content, to study the autotrophic denitrification characteristics of strain W3 and its effect on Fe 2+ utilization. The result is as figure 2 , image 3 shown. From figure 2 , image 3 It can be seen that at pH6.8~7.0, at a temperature of 30°C, NO 3 - -N initial concentration is 40 mg / L, Fe 2+ Under the condition of the initial concentration of 500mg / L, the removal rate of nitrate nitrogen by iron-based autotrophic denitrifying microbacteria W3 can reach 87.0%, and the production of nitrite nitrogen is very...

Embodiment 3

[0027] The iron-substrate autotrophic denitrifying microbacterium W3 was expanded and then put into two identical continuous flow reactors loaded with sponge iron filler. Three weeks after the film-hanging was started, it was found that a layer of biofilm had been attached to the sponge iron filler in the two reactors, and it had a good removal effect on the nitrogen-containing sewage configured in the laboratory. It can be considered that both reactors are hanging The film was successful. Therefore, the influent water of the two biofilm reactors was replaced with pharmaceutical wastewater and battery factory wastewater, and their removal effect on nitrogen in wastewater was monitored after a week of stable operation. The experimental results are shown in Table 1.

[0028] Table 1

[0029]

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Abstract

The invention belongs to the technical fields of bioengineering and environmental engineering, and relates to a denitrifying microbacterium with functions of autotrophic denitrification and ferrous oxidation and application thereof. The bacterial strain described in the present invention is Microbacterium testaceum (Microbacterium testaceum) W3 and the preservation number is: CCTCC NO: M2013108. The invention adopts the pure culture technology of microorganisms to screen out the iron matrix autotrophic denitrifying microbacterium W3 from the natural environment, realizes the preparation of the biological denitrification bacterial agent, and provides highly efficient denitrification bacteria species for the biological reinforcement system. In wastewater treatment, the bacterium is used as a biological denitrification-enhancing functional bacterium to denitrify wastewater through the iron autotrophic denitrification process, with low energy consumption, low investment cost and operation cost, and simple process.

Description

technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering, and relates to an iron matrix autotrophic denitrifying microbacteria strain W3, a screening method thereof and an application in urban sewage treatment under low organic matter concentration. Background technique [0002] An appropriate amount of nitrogen in water is a basic element for the synthesis of algae and other microorganisms, an important material condition for the formation of an aquatic ecological environment, and the basis for a virtuous cycle of the water environment. However, at present, the nitrogen content in some water bodies in our country has exceeded the capacity of the water body. Excessive nitrogen elements lead to abnormal proliferation of algae and other organisms in the water body, causing water quality to deteriorate. At the same time, ammonia nitrogen and nitrite nitrogen are toxic to fish and other organisms. The serious p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
Inventor 王弘宇田俊孙宇翀
Owner WUHAN UNIV
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