90 results about "Periodontal Membrane" patented technology

The invention generally relates to postnatal periodontal ligament stem cells and methods for their use. More specifically, the invention relates in one aspect to postnatal periodontal ligament multipotent stem cells, use of the cells to generate periodontium, differentiation of the cells and methods of tissue cryopreservation.

A cultured periodontal ligament cell sheet is produced by a process comprising culturing cells on a cell culture support composed of a base material whose surface is coated with a temperature-responsive polymer having an upper limit or lower limit critical dissolution temperature in water of 0 to 80° C. under specified conditions, (1) regulating the temperature of a culture fluid to the upper limit critical dissolution temperature or higher or to the lower limit critical temperature or lower, (2) allowing a cultured periodontal ligament cell sheet resulting from the culturing to adhere to a carrier and (3) detaching the sheet intact together with the carrier. This cultured periodontal ligament cell sheet has a syndesmotic microstructure, exhibits extremely high bioadherence to the dental root surface and realizes high density transplantation of target cells and positive reconstruction of periodontal tissues. Moreover, through lamination of cell sheets to be transplanted and donation of a three-dimensional polarity, highly efficient reconstruction of an adhesion apparatus can be accomplished and clinical application thereof to moderate periodontitis, severe peridontitis, gingival recession and the like is highly promising.

The invention relates to a preparation method of a specific extracellular matrix ECM of periodontium. The preparation method comprises the following steps: 1) separating and culturing a human periodontal ligament cell; and 2) preparing the specific extracellular matrix ECM of periodontium; applying the specific extracellular matrix ECM of periodontium and applying the specific extracellular matrix ECM of periodontium as a human periodontal ligament stem cell in in-vitro amplification; and promoting directional differentiation of the ECM. The preparation method is simple and can remarkably promote in-vitro amplification and directional differentiation of the periodontal ligament stem cell.

The invention discloses stem cells for specifically expressing a programmed death receptor 1 (PD-1), and particularly relates to the stem cells from an organic cavity. The stem cells comprise but notlimited to dental pulp stem cells, gingival mesenchymal steam cells (GMSCs), periodontal ligament stem cells (PDLSCs), stem cells of apical papilla (SCAPs) and dental follicle stem cells (DFSCs) or any combination thereof, and preferably comprise stem cells from human exfoliated deciduous teeth (SHED) and / or dental pulp mesenchymal stem cells for permanent teeth (DPSC); the stem cells also includemesenchymal stem cells from other tissues, which do not express the PD-1 at first and express the PD-1 after being modified by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), suchas PD-1 plus bone mesenchymal stem cells (BMMSC) modified by the CRISPR. The invention further discloses an identifying and separating method for the stem cells for specifically expressing the PD-1,a method for preparing the PD-1 plus mesenchymal stem cells modified by the CRISPR as well as application of the mesenchymal stem cells for expressing the PD-1, disclosed by the invention, in tissue regeneration, pain relief and treatment of chronic pain and a series of diseases.

Disclosed is a bionic dental implant. The bionic dental implant is composed of a titanium substrate, a polyurethane permeating porous titanium dioxide layer and a polyurethane layer which serves as a bionic periodontal membrane, and the polyurethane permeating porous titanium dioxide layer is located between the titanium substrate and the polyurethane layer and respectively and closely integrated with the titanium substrate and the polyurethane layer. A preparation method includes that (1) the titanium substrate is subjected to acid treatment, and then micron grade titanium dioxide powders are deposited layer by layer on the surface of the titanium substrate which is subjected to the acid treatment through an electron beam melting technology to form a porous titanium dioxide layer; (2) isophorone diisocyanate and polyethylene glycol or polyoxytetramethylene glycol or polycaprolactone are used as raw materials to prepare a polyurethane prepolymer; (3) the polyurethane prepolymer is coated on the porous titanium dioxide layer, and then the polyurethane layer is formed under the vacuum condition; and (4) a composite with the polyurethane layer on the surface is soaked in a chain extender solution, a catalyst is added in the solution to react, and after the reaction is finished, the composite is taken out of the chain extender solution and naturally dried by airing in the air.

The invention discloses a pulp capping agent with bioactivity and a preparation method thereof. The pulp capping agent with bioactivity comprises dentin extraction solution and dentin powder. The pulp capping agent preparation method comprises the following steps: (1) selecting health dental tissue, cleaning by PBS (Phosphate Buffer Solution) to remove periodontal ligament, cementum and dental pulp, and sterilizing; (2) freezing the dental tissue, and grinding the frozen dental tissue to particles; (3) culturing the dental tissue particles in a medium; and (4) filtering the supernatant from the step (3) to obtain the dentin extraction solution, centrifugally cleaning the cultured dentin particles by the PBS, oven-drying, and grinding to obtain the dentin powders. The pulp capping agent can effectively promote the repair of dentin only by blending the dentin extraction solution with the dentin powder, and applying the mixture at a position close to the dental pulp or at a position at which the dental pulp is exposed, thereby improving the success rate of live-marrow conservation, and providing a new treatment means for patients suffering from accident pulp perforation.

The invention discloses a comprehensive test experiment platform for an orthodontic parodontium mechanical property, which comprises a base plate, a support, a clamping module and a measuring module; the support and the clamping module are arranged on the base plate; the measuring module is arranged on the upper end of the support; the base plate is provided with a longitudinal guide rail and a horizontal guide rail vertical to the longitudinal guide rail; the support and the measuring module are respectively connected with the longitudinal module in a sliding mode; and the clamping module is connected with the horizontal guide rail in a sliding mode. The clamping module of the test experiment platform provided by the invention can regulate the position in a sliding mode; and the uniaxial drawing / compression and the shearing test of a parodontium test sample can be carried out through regulating the fixture type and the relative position of the clamping module and the measuring module, and the parodontium mechanical performance can be tested under the condition of simulating tooth reshaping. The test accuracy of the device provided by the invention is relative high, and the test accuracy requirement can be met; and the device has less components, is simple in structure, is low in processing cost, and is simple to operate.

The invention provides a preparation method of a novel tissue repair-promoting material and an application thereof, particularly can be used for bone defect and repair treatment, which belong to the field of tissue engineering and regenerative medicine. A composite scaffold is prepared by mixing and freeze-drying TGF-beta3-chitosan-ossein protein microspheres and chitosan sponge containing bioactive human-like collagen protein. The composite scaffold has the characteristics of being good in biocompatibility, is capable of promoting proliferation and adhesion of cells around injured tissues andcollecting stem cells in vivo, and is suitable for cell growth and osteogenic differentiation and the like. Meanwhile, the periodontal ligament stem cells can be tightly combined with the scaffold material, so that the good growth of the cells in the scaffold material is facilitated, and the scaffold material has stronger osteogenic differentiation capacity and can be better applied to treatmentof bone defects. Wide application prospects are realized in the fields of bone tissue engineering and regenerative medicine, particularly in the aspects of bone defect treatment and repair and the like.

The present invention provides a protectant of periodontal membranes for inhibiting the destruction of periodontal tissues, particularly periodontal membranes by participation of Porphyromonas gingivalis. Further, it provides an agent for oral cavity or food and drinks containing the protectant of periodontal membranes having effects of prevention and therapy of diseases involving damage of periodontal membranes. A protectant of periodontal membranes is proanthocyanidins, particularly proanthocyanidins derived from apple premature fruits or hop bracts having an effect decreasing the damage to periodontal membranes caused by Porphyromonas gingivalis, and an eating and drinking product containing the protectant of periodontal membranes as an effective component.

The invention relates to a method for extracting the three-dimensional tooth root morphology of an intravital tooth based on periodontal membrane imaging anatomical characteristics. The method comprises the steps of performing imaging scanning on a patient, and storing the scanned data to be a DICOM-format body layer data; importing the DICOM-format body layer data of the patient into Mimics19.0 software, and extracting a discontinuously distributed periodontal membrane mask from the image data; enabling the discontinuous distribution to be expanded into continuous distribution; taking a seedmask as a seed region to expand outwards to the root bone boundary, wherein the maximum expansion step does not exceed an artificially set maximum step parameter; and rebuilding a three-dimensional model of the corresponding tooth, and storing and outputting the three-dimensional model in an STL format to complete segmentation and extraction for the intravital tooth. The method is simple and easyto use, and can quickly realize tissue segmentation for the intravital tooth root.

The invention relates to an orthodontic arch wire with gradually changed orthodontic force value, and a forming technology and device of the orthodontic tooth arch wire. The orthodontic force value and the elastic modulus of the orthodontic arch wire gradually change from a front tooth position to a rear tooth position, so that the orthodontic arch wire can provide a more reasonable orthodontic force value for different teeth of different patients, and the damages of orthodontic force to periodontal membrane tissues are reduced. For the orthodontic tooth arch wire disclosed by the invention, the orthodontic force value is regulated through the change of the cross-sectional shapes of different positions of the orthodontic tooth arch wire and through an aging heat treatment manner, so that the gradual change of the orthodontic force value at different positions of a single orthodontic tooth arch wire is realized.

The invention discloses a stress analysis method for a tooth model for simulating wearing of an appliance. The method comprises: S110, collecting intraoral scanning data, and generating a basic orthodontic model; S120, virtually generating a periodontal ligament geometric model on the basic orthodontic model; S130, determining a periodontal ligament constitutive model, and establishing a finite element model of the periodontal ligament; S140, simulating and calculating the movement amount of teeth by using a finite element method, comparing with the tooth movement conditions acquired by experiments, and determining the to-be-determined parameters in the periodontal ligament constitutive model; S150, obtaining a complete orthodontic model; S160, simulating and calculating a stress distribution condition of the teeth after the teeth are worn with a appliance by using a finite element method; and S170, simulating alveolar bone reconstruction and tooth movement conditions after the teeth are stressed according to the tooth stress distribution conditions. According to the invention, a complete orthodontic model containing periodontal ligament is established, and simulation calculation is carried out on teeth after a appliance is worn, so that the process of orthodontic treatment can be simulated on the biomechanical level.

The invention discloses an oral medicine practice model formed through encapsulation injection molding and a method thereof. The model comprises an alveolar bone formed through injection molding. Multiple alveolar sockets are formed on the top surface of the alveolar bone according to the practice requirements. A locating and connecting structure used for being fixed on an encapsulation mould cavity and a head imitation mould is formed on the bottom surface of the alveolar bone. An encapsulation groove is formed at the circumferential side of the alveolar bone and close to the bottom surface. Soft tissues are formed on the top surface and the circumferential side of the alveolar bone through encapsulation injection molding. The part, which extends to the internal wall of the alveolar sockets, of the soft tissues is provided with periodontal membranes. Teeth are fixed on the periodontal membranes in the alveolar sockets. The alveolar bone is firstly formed through injection molding, and then the soft tissues are formed on the alveolar bone through encapsulation injection molding so that the soft tissues and the alveolar bone are enabled to be evenly and compactly combined, the combination effect is close to the clinical requirements, and the combination quality stability is greatly enhanced. Encapsulation of the alveolar bone is completed in the process of injection molding formation of the soft tissues so that the production efficiency is greatly enhanced, and 1000 models can be formed through encapsulation in 24 hours per day.

Alveolar bone resorption, periodontal membrane loss, and gingival recession can be inhibited by administering 500 mg to 2000 mg per day of calcium, 10 mg to 40 mg per day of soy isoflavone aglycone, and vitamin D3 to persons having a tendency for decreased bone density, postmeopausal women, and periodontal disease patients in a maintenance phase; and even an extended period of administration provides a high degree of safety.

The invention discloses a periodontal ligament stress analysis method and device. The method comprises the following steps: 1, obtaining geometric models of a digital tooth model, a digital periodontal ligament model and a digital alveolar bone model; selecting the types of constitutive models of the three models to form a digital dental model; selecting boundary conditions of the digital dental model to obtain a digital dental finite element model and carrying out nonlinear finite element calculation, and obtaining stress distribution data of the digital periodontal ligament model so as to verify the consistency of the digital tooth model with a simulated correction target when the digital tooth model is rearranged. Whether alveolar bone reconstruction is caused by bone resorption or bonedeposition of the alveolar bone under the conditions that alveolar jaw changes in pose and / or bears load can be accurately judged; the constitutive models closer to periodontal ligament characteristics are selected by utilizing the geometric models closer to the actual situation in the mouth of a patient, so that the calculated stress distribution data is closer to the actual situation, and a data basis is provided for design, preparation and inspection of subsequent dental instruments.

The invention discloses a primary separation and culture method for periodontal ligament stem cells, which comprises the following steps: separating periodontal ligaments, carrying out enzymolysis on periodontal ligament tissues by a mixed enzyme of collagenase V of which the mass-volume concentration is 0.05-0.25% and pancreatin of which the mass-volume concentration is 0.05-0.25%, carrying out primary culture on periodontal ligament stem cells, and separating the periodontal ligament stem cells by adopting a limiting dilution method. The primary separation and culture method disclosed by the invention has the following advantages: (1) the cost is reduced because the cost of the pancreatin is lower than that of dispase; (2) the digestion time is shortened, the periodontal ligaments are dense connective tissues, the affinity of the collagenase V to the connective tissues is superior to that of collagenase I, and the collagenase V and the pancreatin are combined, so that the enzymolysis effect is obviously improved.

The invention provides a preparation method of a regional function specific clinical periodontal defect repair module. The module comprises an alveolar bone regeneration functional domain, a periodontal ligament regeneration functional domain and a barrier membrane functional domain. The method comprises the following steps of firstly, carrying out 3D printing on the personalized magnesium-doped wollastonite alveolar bone regeneration functional domain according to clinical alveolar bone defects; and taking MNBG-modified improved PCL / gelatin electrospun membrane as the periodontal ligament regeneration functional domain and Si-HPMC / MA-CMCS hydrogel formed by photocuring as the barrier membrane functional domain, and integrating the alveolar bone regeneration functional domain, the periodontal ligament regeneration functional domain and the barrier membrane functional domain. Imitation periodontal fiber guide structure design and MNBG modification of the periodontal ligament regeneration functional domain provide guidance and support for secretion and integration of collagen matrixes, and MNBG modification can induce proliferation and vertical migration of periodontal ligament fibroblasts, so that vertical increment of periodontal ligament tissue in a defect area is improved, the regeneration of a periodontal tissue complex is promoted, the determinacy of periodontal tissue regeneration is improved, and the technical sensitivity of a periodontal regeneration operation is reduced.

The invention relates to a bFGF-loaded guided tissue regeneration membrane with a core-shell structure and a preparation method of the bFGF-loaded guided tissue regeneration membrane. The guided tissue regeneration membrane is constructed from basic fibroblast growth factors, synthetic polymer compound polylactic acid-glycolic acid copolymers and natural component wool keratin. The composite membrane provided by the invention can prevent fibroblasts from surrounding tissues from migrating to a defect area, so that periodontal ligament cells preferentially migrate, attach, proliferate and differentiate on the tooth root surface; the sustained release of the growth factor can actively regulate and control the cell function and accelerate the induction of the growth, proliferation and differentiation of cells, so as to obtain more periodontal tissue regeneration. The guided tissue regeneration membrane is simple in preparation method and mild in membrane forming condition, has good cytocompatibility and performance of actively inducing regeneration of periodontal tissues, meets the requirements of in-vivo application, and has good application prospects as a biological membrane with atissue regeneration inducing effect.

The invention provides a making method of a pure titanium tooth, and a method for autotransplanting by means of the pure titanium tooth. The pure titanium tooth is made from Ti, Al, V, N, C, H, Fe and O under the following conditions: the yield strength is 950MPa, the tensile strength is 1005Mpa, the elongation rate is 10%, the elastic modulus is 115Mpa, the density is 4.5g / cm<3>, the melting point range is 1604-16551, and the expansion coefficient is 10.16*10<-6>K<-1>, and the pure titanium tooth having a same shape with a transplantation tooth, printed through a 3D printing technology, is placed in the pre-produced tooth socket of a transplantation site; and the transplantation tooth is used to substitute the pure titanium tooth in the tooth socket after a predetermined time quantum. The method can avoid transplantation tooth periodontal membrane injuries, reduces the inflammation reactions in the transplantation site, and can establish normal periodontal tissues to avoid external root resorption.

The invention discloses an absorbable polylactic acid film for enhancing airtightness of an absorbable root canal post of a deciduous tooth. The absorbable polylactic acid film is prepared by dissolving the following raw materials in parts by weight: 0.5-1.5 parts of polylactic acid, and 5-15 parts of solvent. The absorbable polylactic acid film and the absorbable root canal post are made of the same material, and the absorbable polylactic acid film has no influence on human dental follicle cells, deciduous periodontal ligament cells and deciduous dental pulp cells, cannot result in apoptosis of the human dental follicle cells, is nontoxic, can effectively control the effective strength of the absorbable root canal post, can recover functions and a shape of the deciduous tooth for a long time to the greatest extent, and does not influence normal replacement of deciduous permanent teeth. A preparation method of the film is simple and practicable, and can be used for manufacturing during clinical application. The film is used for residual root and residual crown repair of the deciduous tooth, a crown restoration is good in retention postoperatively, and the film meets the clinical requirement of pediatric dentistry.

The invention provides a method for establishing a dental jaw finite element model for orthodontics, which comprises the following steps: step 1, establishing and optimizing model masks of teeth, dental pulp and alveolar bones by utilizing intraoral CBCT three-dimensional data of a human body; step 2, conducting reverse engineering processing on the three-dimensional reconstruction models of the teeth, the dental pulp and the alveolar bone to complete materialization of the models; step 3, generating a personalized periodontal ligament for each tooth, generating an internal three-dimensional solid structure for the alveolar bone, and assembling all part models; step 4, performing mesh generation on the complete dental three-dimensional solid model, and establishing a complete dental jaw mesh model; and step 5, carrying out independent material assignment on each component in the complete dental jaw mesh model to complete the establishment of the three-dimensional personalized dental jaw model finite element model. The dental jaw finite element model established by the method has extremely high geometric similarity and mechanical similarity, is high in modeling speed, and has greatapplication value in the field of orthodontics.

A jaw simulation model generation method comprises the steps that a jaw model is acquired, and the jaw model at least comprises a tooth body, a periodontal membrane and an alveolar bone; meshing the dental model to obtain a mesh model, the change of the position of each vertex in the mesh model reflecting the change of the geometrical shape of the dental model; endowing each grid unit in the gridmodel with a corresponding initial bone mineral density value; analyzing the geometrical shape change and the bone mineral density change of the grid model in a load state, and meshing the dental model again based on the geometrical shape change and the bone mineral density change of the grid model to obtain a grid model meeting requirements. Due to the fact that bone density and geometrical shapechanges are fused, the jaw simulation model can simulate alveolar bone shape and density changes after teeth move, and the tooth appliance designed and manufactured according to the jaw simulation model can achieve the efficient orthodontic effect.

The invention relates to the technical field of bone defect repair, in particular to a periodontal defect repair system containing Let-7a. The system comprises a third cell polymer and hydroxyapatite-beta tricalcium phosphate wrapped in the third cell polymer; the third cell polymer is transfected with let-7a mimic; the let-7a mimic is a double-stranded RNA, and the sequence of a sense strand of the let-7a mimic is as shown in SEQ ID NO. 2. According to the scheme, the technical problem that the effect of repairing bone defects by using periodontal ligament stem cells is poor can be solved. According to the scheme, the osteogenic differentiation capacity of the periodontal ligament stem cells is regulated and controlled by using Let-7a, and a feasible way is provided for rapid and effective repair of periodontal defects in combination with a periodontal ligament stem cell polymer form cost scheme.

The invention provides a periodontal ligament stem cell proliferation and osteogenic differentiation accelerator, which belongs to the technical field of stem cells. The present invention finds for the first time that the expression level of non-coding RNA RP11-289F5.1 is down-regulated during the osteogenic differentiation of periodontal ligament stem cells. Secondly, the present invention designs and synthesizes shRNA for silencing RP11‑289F5.1, and proves that silencing RP11‑289F5.1 can effectively promote osteogenic differentiation and proliferation of periodontal ligament stem cells.

The invention discloses a method for screening a new subset of periodontal ligament specific stem cells based on single cell sequencing and application thereof. The screened new subset specifically expresses FBLN2 and membrane protein CADM3, and meanwhile, the invention discloses application of the new subset of periodontal ligament specific stem cells in preparation of drugs for treating periodontal diseases. According to the invention, the subset of periodontal ligament specific stem cells is obtained through single cell sequencing, and in vitro flow sorting and cell subset performance verification are carried out through marker genes; According to the invention, the screened cell subset is beneficial to search for intervention targets of periodontal steady-state imbalance, and creates possibility for development of new drugs; periodontal ligament specific stem cells are used as seed cells to form organoids, and a scientific basis is provided for research and verification of new medical instrument materials; and the autologous / allogeneic transplantation feasibility of the periodontal ligament stem cells is further explored, and a new remodeling thought is provided for treating periodontal diseases.

The invention relates to a guided tissue regeneration membrane with a bacteriostatic effect and a preparation method of the membrane. The guided tissue regeneration membrane is constructed by using antibacterial drug ornidazole, drug carrier poly(lactic-co-glycolic acid) and film-forming material wool keratin. The method adopts a high-voltage electrostatic spinning method to compound the antibacterial drug ornidazole with the synthetic polymer compound PLGA and the natural ingredient wool keratin, thereby constructing the composite guided tissue regeneration membrane having excellent physicaland chemical properties, a slow release effect on the ornidazole, good cell compatibility and the bacteriostatic effect. The composite membrane can not only block migration of fibroblasts from surrounding tissue into a defect area, and make periodontal ligament cells preferentially migrated, attached, proliferated and differentiated on the tooth root surface, thereby inducing regeneration of periodontal tissue; the composite membrane can play a bacteriostatic role within 7 days after guided tissue regeneration, prevent the occurrence of postoperative infection caused by bacteria, and ensure the effect of tissue regeneration; and the guided tissue regeneration membrane provided by the invention has the simple preparation method, mild film formation conditions, and good cell compatibility and bacteriostatic performance, and meets the requirements of biological in-vivo application, and has good application prospects as a biological membrane with the bacteriostatic effect.

The invention provides an accelerant for proliferation and osteogenic differentiation of a periodontal ligament stem cell, and belongs to the technical field of stem cells. The accelerant discovers for the first time that the expression quantity of non-coding RNA RP11-289F5.1 in an osteogenic differentiation process of the periodontal ligament stem cell is down-regulated. Secondly, the accelerantdesigns and synthesizes the shRNA of silent RP11-289F5.1, and a result proves that the silent RP11-289F5.1 can effectively accelerate the proliferation and the osteogenic differentiation of the periodontal ligament stem cell.