Application of permanent tooth extract in preparation of mandibular filling material
A technology of filling materials and extracts, applied in the direction of bone implants, etc., can solve the problems of no rejection, high psychological pressure, and difficulty in shaping, and achieve the effects of avoiding rejection, saving costs, and lasting effects.
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Embodiment 1
[0029] Preparation and application of permanent tooth extract
[0030] Use a high-speed turbine to remove periodontal soft tissue, calculus, and carious tissues from extracted teeth or teeth stored in dental banks. Then crush it with a pulverizer, sieve, the size is 425um-850um, remove the enamel layer, manually screen to remove pulp tissue, discolored cementum and dentin particles. Keep the cementum and dentin, and sterilize in a high-temperature steam pot.
[0031] Put the extracted cementum and dentin into a graduated test tube to measure the number of milliliters.
[0032] Put the extracted cementum and dentin into the Bonmaker equipment, pour POWDER reagent respectively, and prepare ATB (dentine bone) in 19 minutes and 50 seconds.
[0033] Surgical method: Perioral disinfection, full-thickness incision of the oral mucosa at the 1-1 vestibular groove transition of the lower anterior teeth, peeling of the periosteum to the mandibular chin process, taking out the ATB, usin...
Embodiment 2
[0037] Dental Bone Bacterial Attachment Test
[0038] Purpose of the experiment: To test the ability of dentin bone used for mandibular filling to resist bacterial attachment.
[0039] Bacteria: Candida albicans, Escherichia coli (purchased from Korean Collection for type Culture).
[0040] Experimental group Experimental materials: Dentin bone ATB was prepared according to the method of Example 1, but before putting into the Bonmaker equipment, the cementum and dentin were contacted with bacteria.
[0041] Experimental materials for the control group: cementum and dentin were prepared according to the method of Example 1, sterilized in a high-temperature steam pot, and then contacted with bacteria without subsequent treatment.
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