Tissue culture method for rapid expanding propagation of tissue culture seedlings for field planting in production process of virus-free minitubers of potatoes

Abstract

The invention provides a tissue culture method for rapid expanding propagation of tissue culture seedlings for field planting in the production process of virus-free minitubers of potatoes. The tissueculture method comprises the steps: (1) ammonium nitrate in an MS culture medium formula is replaced with ammonium chloride, and calcium chloride is replaced with calcium nitrate; (2) carrageenan isreplaced with ginned cotton; (3) the using amount of each mother liquor and white sugar is decreased by 10-30%; (4) at 100 DEG C, after two or more minutes, direct filling is conducted, and the filling amount of a 240-type culture bottle is 20-25ml; (5) expanding propagation is conducted, specifically, mother seedlings are shorn into sections each with 1-2 leaves, after the completion of shearing,cutting is not needed, and gentle scattering and even shaking are conducted; and tissue culture is conducted, specifically, the illumination intensity of tissue culture is 3300 Lx. According to the method, the expanding propagation speed is increased by 30% or above, the tissue culture time is shortened by 7 days, and the cost is reduced by 35% or above.

Description

Technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture formula for rapid propagation of tissue culture seedlings for planting in the production process of potato virus-free minitubers Background technique [0002] Ammonium nitrate is an important component of the existing culture medium. Due to its explosion hazard, it has been banned. After the country bans the sale of ammonium nitrate, it has caused a big problem for plant tissue culture, and it is impossible to obtain the necessary medium. Drugs cannot meet the nutrient elements required by plants, and it is urgent to find substitutes and the amount of substitutes. In addition, mass production of tissue culture seedlings requires a large amount of culture medium. Therefore, how to make culture medium quickly becomes the key. [0003] The production of virus-free minitubers has a strong seasonality, which is directly related to yield and quality. Therefore,...

AI Technical Summary

Problems solved by technology

Large-scale factory production of virus-free potato miniature tubers is faced with the short time for tissue culture seedlings to be planted, and the tissue culture seedlings cannot prepare seeds in advance like other crops, and can only be produced one month in advance, and the operators cannot Just recruit anyone to work and operate. They must be skilled workers. If you maintain a sufficient number of workers all year round, it will be a huge burden and cost for the company.

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