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Device for inoculating wound roots of plants and application method thereof

An inoculator and plant technology, which is applied in the fields of botanical equipment and methods, application, gardening, etc., can solve problems such as not seen, and achieve the effect of simple use method and fast inoculation speed.

Inactive Publication Date: 2015-01-28
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods can be used in laboratories and small-area planted plants. How to ensure the success of inoculation when endophytic bacteria are applied in a large area has not been seen so far.

Method used

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  • Device for inoculating wound roots of plants and application method thereof
  • Device for inoculating wound roots of plants and application method thereof
  • Device for inoculating wound roots of plants and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The acquisition of embodiment 1 plant root endophytic bacteria

[0020] Take the root material of pepper, wash the surface with tap water and dry it, weigh 1g of the root of the plant respectively, soak in 75% ethanol for 3min, rinse with sterile water for 5 times, then soak with 1% sodium hypochlorite for 5min, rinse with sterile water for 5 times . Put the roots into a sterile mortar, add 10 mL of sterile water to each sample, and then dilute to 1×10 -4 , After resting for 15 minutes, take 200μl each and spread on the plate, and repeat 3 times for each treatment. Take 200 μL of sterile water from the last rinse and smear it on the plate to verify whether the disinfection is complete. Incubate in the dark at 28°C for 3-7 days, pick a single colony according to the shape and color of the colony, purify it according to the conventional method, and store it. Results The strains isolated from pepper roots were named LG9 and LJ13.

Embodiment 2

[0021] Embodiment 2 Determination of Plant Endophytic Bacteria Colonization in Roots

[0022] The bacterial strains LG9 and LJ13 isolated from the pepper root that were isolated and purified in Example 1 were used in the literature (edited by Yan Shuzhen and Chen Shuanglin: Microbiology Expansion Experimental Techniques and Methods, Beijing Higher Education Press, published in February 2012, Chapter 4 Experiment 2 ) method for anti-rifampicin labeling, and the labeled strains were formulated into 10 8 cfu / mL bacterial suspension. At the 2-3 leaf stage of pepper seedlings, use a wooden stick to pierce a small hole 2-3cm from the root of the pepper, drop 1mL of bacterial suspension into the small hole, and inoculate 1d, 3d, 5d, 7d and 9d after inoculation. Go out capsicum seedling, stay and get root surface disinfection according to the method for embodiment 1, according to literature (Yan Shuzhen, Chen Shuanglin edited: Microbiology expansion experiment technology and method, ...

Embodiment 3

[0025] The inoculation of embodiment 3 capsicum endophytic bacteria

[0026] Separated from capsicum root according to the method of embodiment 1, adopt embodiment 2 method to prove the colonized endophytic bacterium LG9 bacterial strain, this bacterial strain has anti-rifampicin marker, is formulated into 10 8 Put the cfu / mL bacterial suspension into the WS-16 agricultural sprayer produced by Shandong Weishi Plant Protection Machinery Co., Ltd., remove the nozzle of the sprayer, and replace it with a plant root injury inoculator with a connecting rod length of 8 cm and a cutting head width of 10 mm. . An operator puts the sprayer on his back, and after pressurizing, inserts the inoculator into the soil 5cm above the pepper seedlings (5-6 leaf stage of pepper seedlings), the insertion depth is 5-6cm, and once inserted, the switch of the sprayer is turned on once, that is On and off. Generally, 20-30 pepper seedlings are inoculated per minute. On the 10th day after inoculati...

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Abstract

The invention relates to a device for inoculating wound roots of plants and an application method thereof. The device is connected onto a spray rod of an agricultural sprayer, and is formed by a connecting rod, a cutting head and liquid outlet holes, wherein the connecting rod is of a hollow structure; one end of the connecting rod can be detachably connected with the spray rod of the agricultural sprayer; the other end of the connecting rod is connected with the cutting head; the cutting head is of a hollow structure; the front end of the cutting head is in a wedged shape; a plurality of liquid outlet holes are formed in a wedge-shaped slope; and opening parts of the liquid outlet holes are projected out of the surface of the wedge-shaped slope. According to the device, screws of the connecting rod and screws of the spray rod of the agricultural sprayer are matched, and the use method is simple and convenient. The device is directly fixed on the spray rod without a spray head and is used for inoculating plant endophytes and plant pathogens with infected roots.

Description

technical field [0001] The application of the novel device and application technology of the present invention relates to the field of biological control of pests and diseases in agricultural production, and the novel inoculator of the present invention can be used to inoculate plants with endogenous biocontrol bacteria and growth-promoting bacteria. Background technique [0002] Plant endophytes are those bacteria that live in various tissues and organs of healthy plants or in the intercellular space during a certain stage or all stages of their life history. According to the effect on the host plant, plant endophytes can be divided into beneficial endophytes, harmful endophytes and neutral endophytes, among which neutral endophytes account for the vast majority. In recent years, because endophytes exist in plants and are not easily affected by the external environment, they have biological effects on host plants such as growth promotion, disease prevention, and endogenous ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 闫淑珍陈双林
Owner NANJING NORMAL UNIVERSITY
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