Method for producing oyster mushroom strains from birch chopsticks
A technology of chopsticks and strains, applied in the field of preparation of strains of Pleurotus ostreatus, can solve the problems of complex inoculation procedures, long cultivation period, and low inoculation efficiency, and achieve simplified culture solution components, low production costs, and rapid growth of strains Effect
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[0011] The method for producing Pleurotus ostreatus strains with disposable birch chopsticks is divided into four steps: pretreatment of chopsticks, preparation of chopstick culture medium, inoculation and culture, and transfer of strains. (1) Chopsticks pretreatment Remove bent and cracked defective products from disposable birch chopsticks, sharpen the forks of the chopsticks, immerse them in 5% lime water for 20-24 hours, then immerse the chopsticks in clean water, pass through 1500mg / h ozone for 30-60 minutes; (2) Preparation of chopsticks culture medium Immerse the pretreated chopsticks in 5.5% sucrose, 1.9 glucose, 0.12% V B1 , 0.08% of V B9 , 0.2% potassium dihydrogen phosphate, and 0.2% magnesium sulfate aqueous solution, the water temperature is kept at 50-70 ° C, after soaking for 1-2 hours, remove and drain the water, and when the surface is slightly dry, put 50% In the mixture of bran and 50% corn flour, stir evenly, so that the chopsticks are covered with the mi...
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