Special inoculation frame for liquid cell culture

Abstract

The invention relates to microbial culture and in particular relates to a special inoculation frame special for liquid cell culture. The inoculation frame comprises a base plate and a supporting plate of which the bottom is in running fit with the base plate, wherein a baffle plate is arranged at the rear part of the upper surface of the supporting plate, a bottleneck fixing mechanism is arranged at the front part of the upper surface of the supporting plate, and an angle adjusting device is arranged between the supporting plate and the base plate. The inoculation frame provided by the invention can be used for solving the technical problems of low inoculation speed, high operation strength, high contamination probability and the like of the prior art, and has the advantages of being stable in supporting structure, suitable for clamping fixation of triangular flasks with different specifications, high in inoculation speed, high in effect, saving in labor and the like.

Description

technical field [0001] The invention relates to microorganism culture, in particular to a special inoculation rack for liquid cell culture. Background technique [0002] Microbial inoculation is a basic operating technique in microbiological research. In microbiological experiments and fermentation production, it is necessary to transfer microorganisms to another freshly sterilized medium to grow and reproduce and obtain metabolites according to different culture purposes. Slant inoculation, liquid inoculation, etc. can be used, but the inoculation has not been able to realize automatic operation at present. [0003] During the fermentation process, bacteria often adopt a mechanism of step-by-step amplification to enhance the activity and adaptability of the bacteria, and finally carry out rapid and efficient fermentation to produce and accumulate a large amount of target products in the bacteria or in the fermentation broth. Gradual amplification means to carry out primary...

AI Technical Summary

Problems solved by technology

This kind of operation, because the bottle mouth is directly exposed to the air upwards, firstly, it increases the probability that the bacteria in the air are directly mixed into the seed liquid, and in addition, due to the limited space of the ultra-clean workbench, or the limitation of the height of the protective glass, It also increases the difficulty of operating pipettes (such as pipettes) in the workbench

For the above problems, the solution currently used is two-person operation. One person holds the seed bottle and always points the mouth of the seed bottle at the flame, and the other holds the fresh culture solution bottle and is responsible for the suction and injection of the seed solution. This cooperation can maintain The bottle mouth of the seed solution is inclined to reduce the chance of infection and speed up the inoculation speed, but it consumes manpower and reduces the inoculation efficiency

Transplantation or expansion inoculation in large-scale production is carried out in a sterile room. With the increase in the number of operators, the chance of bringing in miscellaneous bacteria has also greatly increased. Therefore, it is urgent to design an inoculation device to solve the problem of large-scale production. It is very necessary for a single person to complete the inoculation operation of a large-capacity triangular flask

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