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Pleurotus edible fungus rapid proliferation nutrient solution and pretreatment method of cultivated species of nutrient solution

A technology of nutrient solution and edible fungus, which is applied in cultivation, plant cultivation, mushroom cultivation, etc. It can solve the problems of reducing the inoculation speed, slowing down the growth speed of mycelia, and infection, and achieves fast inoculation speed, fast recovery growth speed, The effect of low equipment investment cost

Active Publication Date: 2016-12-07
MICROBIOLOGY RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While radiation treatment and chemical fumigation kill microorganisms in the air, they also cause a certain degree of harm to the body of the vaccinators. At the same time, the investment cost of ultra-clean workbench is high, which is unaffordable for ordinary mushroom growers.
[0004] (2) Due to the limited working interface of the ultra-clean workbench and the inoculation box, the bacteria bag enters and exits the ultra-clean table and the inoculation box during the inoculation operation, and it takes a lot of time to open and seal the bacteria bag during inoculation, which reduces the inoculation speed
[0005] (3) When the cultivars whose surface is covered with bacterial species are placed on a new medium, the mycelium will be damaged to a certain extent due to the cutting of the bacterial block during inoculation, and an adaptation period of 5-7 days is required on the new medium. The recovery of growth not only prolongs the cultivation time of mycelium, but also provides opportunities for the infection of miscellaneous bacteria; at the same time, because the bag mouth is sealed by stuffing cotton, the air permeability is poor, resulting in slower growth of mycelium in the later stage

Method used

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  • Pleurotus edible fungus rapid proliferation nutrient solution and pretreatment method of cultivated species of nutrient solution
  • Pleurotus edible fungus rapid proliferation nutrient solution and pretreatment method of cultivated species of nutrient solution
  • Pleurotus edible fungus rapid proliferation nutrient solution and pretreatment method of cultivated species of nutrient solution

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1 Pleurotus eryngii production application

[0029] (1) Culture medium preparation

[0030] Cultivar culture medium (for cultivation of cultivar mycelium)——recipe: every 1000g medium contains 320g of mulberry branches, 250.0g of bagasse, 200.0g of cottonseed hulls, 150g of corncobs, 50g of rice bran, 10.0g of gypsum powder, Calcium phosphate 10.0g, trehalose 10g, chlorogenic acid 0.05g, the balance is water; preparation method: weigh each component according to the mass, and mix mulberry branches, bagasse, cottonseed hulls, corncobs, rice bran and gypsum powder into 6 components Mix and mix well to form a mixture, dissolve calcium superphosphate, trehalose, and chlorogenic acid in appropriate amount of water to form a mixed solution, mix the mixture and the mixed solution, and finally add the remaining water and stir evenly to obtain the product.

[0031] Nutrient solution for mycelium rapid proliferation (for pretreatment of mycelia of cultivated species) -...

Embodiment 2

[0048] Embodiment 2 conventional inoculation method and comparative test of the present invention

[0049] Conventional inoculation method 1 (conventional 1 for short): adopt the cultivar cultivated by the formula of conventional cultivar, and inoculate on the ultra-clean workbench;

[0050] Conventional inoculation method 2 (conventional 2 for short): adopt the cultivar cultivated by conventional cultivar formula, inoculate in artificial inoculation box;

[0051] Conventional inoculation method 3 (abbreviated as routine 3): cultivars cultivated with conventional cultivar formulas are inoculated openly in a disinfection room;

[0052] The inoculation method of the present invention (abbreviated the present invention).

[0053] Among them, the cultivar formula used in the conventional inoculation method: 32.0% of mulberry branch, 25.0% of bagasse, 20.0% of cottonseed hull, 15.0% of corncob, 5.0% of rice bran, 1.0% of gypsum powder, and 1.0% of superphosphate; Mushroom bag for...

Embodiment 3

[0063] Example 3 Production and application of phoenix tail mushroom

[0064] (1) Culture medium preparation

[0065] Cultivar culture medium (for cultivation of cultivar mycelium) - formula: every 1000g medium contains 220g of mulberry branches, 250.0g of bagasse, 250.0g of cottonseed hulls, 150g of corncobs, 60g of rice bran, 50g of peat soil, and gypsum powder 10.0g, calcium superphosphate 10.0g, trehalose 10g, chlorogenic acid 0.05g, the balance is water; preparation method: weigh each component according to the mass, and mix mulberry branches, bagasse, cottonseed hulls, corncobs, rice bran, peat Mix the 6 ingredients of soil and gypsum powder to form a mixture, dissolve superphosphate, trehalose, and chlorogenic acid in an appropriate amount of water to form a mixed solution, mix the mixture and the mixed solution, and finally add the remaining water and stir evenly, that is have to.

[0066] Nutrient solution for mycelium rapid proliferation (for pretreatment of myceli...

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Abstract

The invention discloses a pleurotus edible fungus rapid proliferation nutrient solution, each 1,000 ml of hyphae rapid proliferation nutrient solution comprises 5 g of saccharose, 5 g of trehalose, 1 g of glucose, 0.05 g of chlorogenic acid, 0.05 g of VB2, 0.05 g of VB6 and the balance of water, and the PH is 7.0. Accordingly, the invention further discloses a pretreatment method of corresponding cultivated species. The proliferation nutrient solution added with different sugars, the vitamins and the antibacterial agent is used for pretreatment on cultivated specie mycelium, the fungus vigor is enhanced, the setting time of mycelium is shortened, the anti-bacterial capability of the mycelium is improved, and therefore, and the sundry fungus pollution rate is reduced while an open type inoculation method is ensured to be adopted. At the same time, a stapler breathable type sealing manner is adopted, oxygen supply during out-of fungus bag culture is enhanced, and the mycelial growth cycle is shortened. Rapid culture, high yield and harmlessness of the pleurotus edible fungus can be realized.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a nutrient solution for rapid proliferation of edible fungi of the genus Pleurotus and a pretreatment method for the cultivated species thereof. Background technique [0002] The inoculation of edible fungus mushroom bags is an important technical link in the production of edible fungi. The inoculation speed and inoculation quality are related to the yield of mushroom bags, mycelium growth speed, quality and final biological conversion rate. At present, the commonly used mushroom bag inoculation methods mainly include ultra-clean workbench inoculation and manual inoculation box inoculation. Their shortcomings are: [0003] (1) The inoculation site needs to irradiate the air with ultraviolet light or fumigate with formaldehyde and phenol every day. While radiation treatment and chemical fumigation kill microorganisms in the air, they also cause a certain degre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
CPCA01G18/00C05B1/02C05F11/00C05G3/00C05G5/23C05D3/00C05F5/002C05F11/02
Inventor 陆荣生韩美丽胡小芬梁志强苏启臣杨迪
Owner MICROBIOLOGY RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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