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Method for producing shell strains by using shell-shaped strain inoculator

An inoculator and strain technology, applied in botany equipment and methods, applications, horticulture, etc., can solve the problems of reduced product yield and quality, decreased mycelium vigor, increased pollution rate, etc., to reduce production costs and growth cycle The effect of shortening and reducing the pollution rate

Inactive Publication Date: 2012-08-15
沈阳蕈丰食用菌科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the age difference of the sawdust bacteria is obvious, the growth rate of the age of the upper, middle and lower parts is inconsistent, the interior is easy to contain miscellaneous bacteria, which increases the pollution, the culture period is too long, the growth period of the bacteria is 40-50 days, and the mycelial vitality decreases. It may lead to cultivation failure; although the growth rate of granular strains is fast, the production cost is high, and the requirements for the production of strains are strict, so they cannot be used for inoculation by punching holes; branch strains are all processed from the core material of trees. Soaking is required before production, and this process is difficult to grasp, which often results in insufficient mycelium content in the branches, resulting in the failure of germination. post-growth slow
Therefore, conventional strain production methods may lead to a decrease in the mycelial activity of edible fungi, an increase in the pollution rate, and a reduction in product yield and quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 A kind of method that adopts shell-shaped strain inoculator to produce shiitake mushroom shell strain

[0014] Proceed as follows:

[0015] 1) The culture medium formula of the cannonball strain: taking the production of 10,000 bags of mushroom strains as an example, 40,000 shell-shaped strain inoculators are required; main material: fine wood chips 160 kg; auxiliary materials: 30 kg of wheat bran, 3.6 kg of cornmeal , gypsum 1.8 kg, potassium dihydrogen phosphate 0.4 kg, magnesium sulfate 0.2 kg, peptone 1.8 kg, glucose 1.8 kg;

[0016] 2) Mixing material: add water to the weighed main material and auxiliary material and mix well, adjust to 62% water content, and prepare the culture medium; into the medium, and the remaining medium that is not stirred into the inoculator is used for later use;

[0017] 3) Bottling: put the inoculum mixed into the medium into 800 bottles, and each bottle is filled with 50 inoculums. When bottling, sprinkle a small amoun...

Embodiment 2

[0021] Embodiment 2 A kind of method that adopts shell-shaped bacterial spawn inoculator to produce slide mushroom shell bacterial strain

[0022] Proceed as follows:

[0023] 1) The culture medium formula of the cannonball strain: taking the production of 10,000 bags of slide mushroom strains as an example, 40,000 shell-shaped strain inoculators are required; main material: 160 kg of fine wood chips; auxiliary materials: 30 kg of wheat bran, 3.6 kg of cornmeal kg, gypsum 1.8kg, lime 1.2kg, potassium dihydrogen phosphate 0.4 kg, magnesium sulfate 0.2 kg, peptone 2 kg, glucose 2 kg;

[0024] 2) Mixing material: add water to the weighed main material and auxiliary material and mix well, adjust to 65% water content, and prepare the culture medium; into the medium, and the remaining medium that is not stirred into the inoculator is used for later use;

[0025] 3) bottling: with example 1;

[0026] 4) Sterilization: same as example 1;

[0027] 5) Inoculation culture: Under as...

Embodiment 3

[0029] Embodiment 3 A method of producing black fungus shell strains using a shell-shaped strain inoculator

[0030] Proceed as follows:

[0031] 1) The medium formula of the cannonball strain: Take the production of 10,000 bags of black fungus strains as an example, 20,000 shell-shaped strain inoculators are required. Main ingredients: fine sawdust 80kg; auxiliary materials: wheat bran 15kg, corn flour 1.8 kg, gypsum 0.9 kg, lime 0.8kg, potassium dihydrogen phosphate 0.2kg, magnesium sulfate 0.1 kg, peptone 1 kg, glucose 1 kg;

[0032] 2) Mixing material: add water to the weighed main material and auxiliary material and mix well, adjust to 60% water content, and prepare the culture medium; into the medium, and the remaining medium that is not stirred into the inoculator is used for later use;

[0033] 3) bottling: with example 1;

[0034] 4) Sterilization: same as example 1;

[0035] 5) Inoculation culture: Under aseptic operation, open the mouth of the bottle, insert b...

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PUM

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Abstract

The invention relates to a method for producing shell strains by using a shell-shaped strain inoculator. According to the technical scheme, a formula of a culture medium of the shell strains is composed of a main ingredient and accessories, wherein on the basis of 10000 shell-shaped strain inoculators, the main ingredient is 30-50kg of sawdust; and the accessories are composed of: 7-8kg of wheat bran, 0.5-1.5kg of corn flour, 0.2-0.6kg of gypsum, 0-0.5kg of lime, 0.1-0.2kg of monopotassium phosphate, 0.05-0.1kg of magnesium sulfate, 0.4-0.5kg of peptone and 0.4-0.5kg of glucose. After the main ingredient and accessories are stirred, filled into bottles, sterilized and subjected to inoculated culture, the shell strains are obtained. Compared with a conventional solid strain, the method provided by the invention has the advantages that the operation is convenient, the strain age is consistent, the strain purity is high and the seed applying cost is reduced; the time for culturing hyphaeis shortened by 10-15 days; and when a culturing bag is produced, the inoculation survival rate is increased to 100%, the pollution rate is reduced by 5% and the yield is increased by 5-10%.

Description

technical field [0001] The invention relates to a production method of edible fungus strains, in particular to a method for producing cannonball strains by using a shell-shaped strain inoculator. Background technique [0002] The conventional production method of existing edible fungus, the edible fungus strain that adopts has four kinds such as sawdust, granule, branch, capsule. Among them, the age difference of the sawdust bacteria is obvious, the growth rate of the age of the upper, middle and lower parts is inconsistent, the interior is easy to contain miscellaneous bacteria, which increases the pollution, the culture period is too long, the growth period of the bacteria is 40-50 days, and the mycelial vitality decreases. It may lead to cultivation failure; although the growth rate of granular strains is fast, the production cost is high, and the requirements for the production of strains are strict, so they cannot be used for inoculation by punching holes; branch strain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 张季军付兴亮张敏肖千明孙利平刘娜李红张晓黎刘俊杰张士义马玉珠刘国宇宋莹刘岩岩
Owner 沈阳蕈丰食用菌科技有限公司
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