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Preparation method and application of fungal suspension

A bacterial suspension and fungal technology, applied in the biological field, can solve the problems of cumbersome steps, invisible pollution, time-consuming, etc., and achieve the effect of simple and convenient operation, strong permeability and fast germination

Inactive Publication Date: 2017-02-15
SHENZHEN ZEQINGYUAN TECH DEV SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this liquefied strain has good vigor, strong permeability, many germination points, and high yield, but because the liquefaction of this technology is a solid strain, there is a high probability of invisible pollution, and the steps before liquefaction by this technology are cumbersome and time-consuming Longer, higher cost

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0027] This embodiment discloses a kind of preparation method of fungus (oyster mushroom) bacterium suspension, described method comprises the steps:

[0028] (1) Preparation of liquid strains: firstly prepare 300 mL of "corn flour + bean cake powder" liquid medium, divide into 250 mL Erlenmeyer flasks, 100 mL per bottle, and sterilize under high pressure steam at 121°C for 30 minutes. After cooling, take 2-3 pieces of soybean-sized test tube mother seeds without medium to inoculate, culture on a shaker, culture conditions: temperature: 20-25°C, speed: 180-220rpm. The liquid strain can be obtained after culturing for 7 days;

[0029] (2) Filtration and elution: pass the liquid strain obtained in step (1) through a 10-mesh sieve, and elute 3 times with sterile water to obtain 45 g of pure mycelium balls;

[0030] (3) Add water and pulverize: add the pure mycelia ball obtained in step (2) into a sterile pulverizer, add 4500 mL of sterile water, pulverize for about 1 minute, and...

Embodiment 2

[0038] This embodiment discloses a kind of preparation method of fungus (flammulina velutipes) bacterial suspension, described method comprises the steps:

[0039] (1) Preparation of liquid strains: firstly prepare 300 mL of "corn flour + bean cake powder" liquid medium, divide into 250 mL Erlenmeyer flasks, 100 mL per bottle, and sterilize under high pressure steam at 121°C for 30 minutes. After cooling, take 2-3 pieces of soybean-sized test tube mother seeds without medium to inoculate, culture on a shaker, culture conditions: temperature: 20-25°C, speed: 180-220rpm. The liquid strain can be obtained after culturing for 7 days;

[0040] (2) Filtration and elution: pass the liquid strain obtained in step (1) through a 10-mesh sieve, and elute 3 times with sterile water to obtain 45 g of pure mycelium balls;

[0041] (3) Add water and pulverize: add the pure mycelia ball obtained in step (2) into a sterile pulverizer, add 4500 mL of sterile water, pulverize for about 1 minute...

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Abstract

The invention belongs to the field of biology and particularly relates to a preparation method and application of a fungal suspension. The preparation method includes the steps of 1), preparing a liquid strain; 2), filtering and eluting; 3) adding water and grinding so as to obtain the fungal suspension. The fungal suspension has the advantages of strong mycelia vigor, many germination points, fungus age consistency, short production cycle, small pollution risks, low production cost and good economic benefit. The preparation method has the advantages that the defects of traditional solid strains, liquid strains and existing solid liquefied strains are overcome. The fungal suspension can be used for preparing liquid strains except for production and has a promising market prospect and huge economic value.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a preparation method and application of a fungal suspension. Background technique [0002] Basidiomycetes have a well-developed mycelium with a septum. At present, basidiomycetes, especially edible fungus strains, mainly exist in two forms: solid strains and liquid strains. Although solid strains are easy to inoculate, they are slow to develop, the culture period is long, and there is a high probability of invisible pollution. Although the liquid strains germinate quickly and the culture period is short, due to the high sugar content, the strains are relatively viscous, the permeability is not good, and the probability of large-scale contamination is high. [0003] At present, in addition to the traditional solid and liquid strains, there is also a special strain - solid liquefied strains. The specific preparation method is as follows: first prepare the liquid bacteria, then...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L31/00C12R1/645
CPCC12N1/14
Inventor 彭志立黄梦婷陈强
Owner SHENZHEN ZEQINGYUAN TECH DEV SERVICE CO LTD
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