Method for quickly detecting whether or not schizophyllum commune liquid strain reaches standard and verification method of method
A technology of liquid strains and schizophyllum, which is applied in the direction of microorganism-based methods, botany equipment and methods, and microbial measurement/inspection, can solve the problem of high professional quality requirements for testing personnel, which is unfavorable for large-scale production and testing. Low efficiency and other problems, to achieve the effect of vigorous growth of mycelia, fast detection method, and short cultivation time
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[0023] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used can be purchased from commercial sources unless otherwise specified.
[0024] The method for quickly detecting whether the liquid strain of schizophyllum reaches the standard comprises the following steps:
[0025] (1) Preparation of liquid medium: the medium includes the following components: 2% bran, 1.5% glucose, 0.2% peptone, KH 2 PO 4 0.2%, MgSO 4 0.15%, the rest is water; accurately weigh, boil the bran juice, filter to remove residue, and constant volume. The liquid culture medium determined by the present invention is the premise of realizing the object of the present invention,
[0026] (2) Expanded cultivation of liquid strains: Prepare the medium according to the size of the fermenter, put the liquid strain of Schizophyllum liquid into the fermenter filled with the medium, sterilize at 121°C, 0.1Mpa for 40min, and ...
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