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Method of determining aflatoxin and pesticide residues in peanut by ultra performance liquid chromatography-quadrupole/high resolution mass spectrometry

A kind of aflatoxin, ultra-high performance liquid phase technology, applied in the field of analytical chemistry, to achieve the effect of fast detection method, elimination of matrix interference, and improved accuracy

Inactive Publication Date: 2019-06-25
INSPECTION & QUARANTINE TECH CENT OF YANTAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Problems solved by technology

There are no reports in the literature on the simultaneous detection of aflatoxins and pesticide residues in peanuts using high-resolution mass spectrometry

Method used

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  • Method of determining aflatoxin and pesticide residues in peanut by ultra performance liquid chromatography-quadrupole/high resolution mass spectrometry
  • Method of determining aflatoxin and pesticide residues in peanut by ultra performance liquid chromatography-quadrupole/high resolution mass spectrometry
  • Method of determining aflatoxin and pesticide residues in peanut by ultra performance liquid chromatography-quadrupole/high resolution mass spectrometry

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Experimental program
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Effect test

Embodiment

[0022] 1 Instruments and reagents

[0023] Quadrupole / electrostatic field orbitrap high-resolution mass spectrometer Q-Exactive (ThermoFisher Scientific); UltiMate 3000 fast liquid chromatography (ThermoFisher Scientific); 3K15 centrifuge (Germany Sigma); DL-5C low-speed large-capacity centrifuge Machine (Shanghai Anting Scientific Instrument Factory); OA-SYS Nitrogen Blowing Apparatus (ORGANOMATION, USA); Milli-Q Ultrapure Water Purification System (Millipore, USA), Vortex Mixer (IKA, Germany); Milli-Q Ultra Pure water (Millipore, USA); 0.2 μm PTFE membrane syringe filter (PALL).

[0024] Aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2) standard solution (0.5-2 μg / mL, TRILOGY company); Acetochlor, carbendazim, carbofuran, chlorpyrifos, difenoconazole, dimethomorph, imidacloprid, pyrimethanil, tebuconazole, thiamethoxam (Scientific Monitoring Institute for Environmental Protection of the Ministry of Agriculture, 1000mg / L ); Acetonitrile, met...

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Abstract

The invention relates to a method of detecting aflatoxin and pesticide residues in a peanut, in particular to a method of determining aflatoxin and pesticide residues in a peanut by ultra performanceliquid chromatography-quadrupole / high resolution mass spectrometry, which belongs to the field of analytical chemistry. A method of determining four kinds of aflatoxin and eleven kinds of pesticide residues in a peanut by ultra performance liquid chromatography-quadrupole / high resolution mass spectrometry adopts high performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry to determine aflatoxin and pesticide residues in a peanut, a sample is extracted through an acetic acid-acetonitrile-water extract and is purified through a PSA and C18 silica gel mixed system, an aqueous solution containing 0.1% of formic acid and methanol are used in a positive ion mode to perform gradient elution on a mobile phase, and high resolution mass spectrometry qualitative and quantitative detection is carried out. The method is accurate, efficient and reproducible, and can simultaneously determine four kinds of aflatoxin and eleven kinds of pesticide residues in a peanut.

Description

technical field [0001] The invention relates to a method for detecting aflatoxin and pesticide residues in peanuts, in particular to a method for determining 4 kinds of aflatoxins and 11 kinds of pesticide residues in peanuts by ultra-high performance liquid chromatography-quadrupole / high-resolution mass spectrometry , which belongs to the field of analytical chemistry. Background technique [0002] Peanut is one of the important grain, oil and economic crops in my country, accounting for about 60% of the world's total peanut production. The total output of peanuts and peanut oil in my country is far ahead of other countries in the world, and China has become the world's largest peanut exporter. With the development of peanut import and export trade, mycotoxins and pesticide residues are increasingly used as technical trade barriers for its import and export, which also poses challenges to our detection technology. [0003] Aflatoxin (aflatoxin) is extremely toxic, mainly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 胡巧茹曹鹏丛中笑梁君妮沙美兰李晓玉尹大路于杰
Owner INSPECTION & QUARANTINE TECH CENT OF YANTAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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