Method and kit for detecting forbidden antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry and application

Abstract

The invention discloses a method and a kit for detecting forbidden antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry and application. The method comprises the following steps: firstly, establishing an ion mobility spectrometry library of antibacterial drugs to be detected in a sample to be detected; then extracting a sample, and purifying and concentrating the sample through cation solid-phase extraction; then determining components of the sample by adopting electrospray-ion mobility spectrometry; and finally, judging whether the sample contains the forbidden antibacterial drug or not by using an ion mobility spectrometry library or comparing migration time. The matched kit comprises reagents and experimental apparatuses required by sample pretreatment. According to the method disclosed by the invention, an optimized sample pretreatment method is adopted, so that matrix interference in cosmetics can be effectively eliminated, and occurrence of false positive is reduced; the inhibition effect of the surfactant in the cosmetics is obviously reduced, and the extraction solution is concentrated, so that the occurrence of false negative is reduced. The kit is simple to operate, easy to master and carry, does not need professionals and precise pretreatment instruments, and is suitable for on-site screening.

Description

technical field [0001] The invention belongs to the technical field of cosmetic detection, and in particular relates to a method, kit and application for detecting prohibited antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry. Background technique [0002] With the gradual development of the national economy, people's pursuit of beauty is getting higher and higher, and the demand for cosmetics is also increasing. In order to achieve good results and quick results, illegal businesses illegally add various prohibited chemicals to cosmetics, causing consumers There are some adverse reactions after use. Among them, antibacterial drugs mainly include antifungal drugs and synthetic antibiotics, which belong to prescription drugs and are randomly added to cosmetics, which can easily cause contact dermatitis, manifested as erythema, edema, erosion, desquamation, exudation, itching, burning and other symptoms, and even cause serious health problems. Ac...

AI Technical Summary

Problems solved by technology

Microwave extraction has a good purification effect on cosmetic matrix, but it requires expensive instruments and digestive tubes, and the operation of the instrument consumes a lot of power, so it is not suitable for on-site inspections; techniques such as liquid phase microextraction and solid phase microextraction are easy to use, but the sample load is relatively large. Low, due to the use of less solvents, it is necessary to use different solvents to match the properties of cosmetics in order to achieve fully dispersed samples and accurate quantitative results, and the specificity is poor, often does not solve the specificity and reproducibility caused by the pollution caused by the cosmetic matrix question

Solid phase extraction (solid phase extraction, SPE) is a relatively mature sample purification and enrichment technology, but there is no mature and general method in the prior art that matches ESI-IMS on-site screening and determination of antibacterial drugs in cosmetics; at the same time, There are no professional inspectors and experimental origins for on-site inspections, and the development of portable and simple cosmetic purification kits is also of positive significance, truly realizing the seamless connection between quick inspection and supervision

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