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Pleurotus eryngii liquid strain culture medium and preparation method thereof

A technology of liquid strains and medium, applied in fertilizer mixture, fertilization device, application, etc., can solve the problems of raw material consumption and cumbersome operation

Inactive Publication Date: 2014-01-15
SHANGHAI ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other liquid medium methods have been reported before, such as potatoes as a carbon source and bran as a nitrogen source, but this method is more cumbersome to operate in the actual factory production process and consumes raw materials

Method used

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  • Pleurotus eryngii liquid strain culture medium and preparation method thereof
  • Pleurotus eryngii liquid strain culture medium and preparation method thereof
  • Pleurotus eryngii liquid strain culture medium and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0022] The preparation of embodiment one culture medium, wherein the formula of some components is shown in Table 1.

[0023] The partial formula composition of table 1 culture fluid

[0024]

[0025] ① Put the sugar into the container, add 10L of water, stir to dissolve;

[0026] ②, add MgSO 4 and KH 2 PO 4 and dissolve it;

[0027] ③. Filter the bean paste powder through a 120-mesh sieve, and then add it to the above solution to fully disperse it in water;

[0028] ④. Filter the above liquid through 50-100 mesh nylon cloth, pour it into a fermenter, add 50ml of emulsified silicone oil; add water to make up to volume.

[0029] Pour the prepared culture medium into the fermenter, and sterilize it with high-pressure steam at 121°C for 90 minutes. When the temperature is cooled below 85°C, push out the fermenter and inject sterile air at a pressure of 0.5-0.1 MPa. And place a water drench on the tank body to cool the tank body rapidly. When the tank body temperature is ...

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Abstract

The invention discloses a pleurotus eryngii liquid strain culture medium and a preparation method thereof. The culture medium consists of white sugar, soya bean meal, MgSO4, KH2PO4, emulsified silicone oil and water.

Description

technical field [0001] The invention belongs to the technical field of fungal fermentation, and in particular relates to a liquid culture medium of Pleurotus eryngii and a preparation method thereof. technical background [0002] Pleurotus eryngii (PleurotuseryngiiQuel.), also known as celery, is rich in nutrients, rich in protein, carbohydrates, vitamins and minerals such as calcium, magnesium, copper, zinc, etc. It has the functions of moisturizing the stomach and beautifying, and has extremely high cultivation value and commercial value. [0003] At present, the cultivation of Pleurotus eryngii at home and abroad is dominated by solid strains, while liquid strains have the advantages of short production cycle, consistent bacterial age, complete mushroom production, easy management, low cost of strains, and convenient and rapid inoculation compared with solid strains. , which is conducive to the scale and industrialization of edible fungi production. However, the technic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 谭琦王涛鲍大鹏李正鹏周峰李玉
Owner SHANGHAI ACAD OF AGRI SCI
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