Pleurotus eryngii liquid strain culture medium and preparation method thereof
A technology of liquid strains and medium, applied in fertilizer mixture, fertilization device, application, etc., can solve the problems of raw material consumption and cumbersome operation
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[0022] The preparation of embodiment one culture medium, wherein the formula of some components is shown in Table 1.
[0023] The partial formula composition of table 1 culture fluid
[0024]
[0025] ① Put the sugar into the container, add 10L of water, stir to dissolve;
[0026] ②, add MgSO 4 and KH 2 PO 4 and dissolve it;
[0027] ③. Filter the bean paste powder through a 120-mesh sieve, and then add it to the above solution to fully disperse it in water;
[0028] ④. Filter the above liquid through 50-100 mesh nylon cloth, pour it into a fermenter, add 50ml of emulsified silicone oil; add water to make up to volume.
[0029] Pour the prepared culture medium into the fermenter, and sterilize it with high-pressure steam at 121°C for 90 minutes. When the temperature is cooled below 85°C, push out the fermenter and inject sterile air at a pressure of 0.5-0.1 MPa. And place a water drench on the tank body to cool the tank body rapidly. When the tank body temperature is ...
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