A kind of method for producing tea tree mushroom strain by liquid culture and preservation method thereof
A liquid culture, tea tree mushroom technology, applied in the fields of botany equipment and methods, horticulture, application, etc., can solve the problems of inconsistent bacterial age, long cultivation period and high production cost, and achieve the effect of reducing production cost.
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Embodiment 1
[0024] The present invention provides a kind of method of liquid culture production tea tree mushroom strain, and its process step and process parameter are:
[0025] 1. Liquid culture efficient strain screening
[0026] It is preferred to culture a strain with a large number of bacterial balls, uniform size, and many burrs on the surface, the number of bacterial balls is 200-260 / ml, and the diameter of bacterial balls is 1.0-1.5ml;
[0027] 2. shake flask culture
[0028] ① Shake flask medium formula: glucose 1-3%, corn flour 2-4%, yeast powder 0.2-0.4%, bran 1.0-3.0%, KH 2 PO 4 0.5-1.5%, MgSO 4 0.5-1.0%, PH value is natural.
[0029] ② Shake flask culture medium preparation: wash the shake flask first, then empty it, and the sterilization method is the same as the conventional one. Weigh the raw materials according to the shake flask medium formula, boil the potato slices for 10-15 minutes, filter and take the filtrate, boil the wheat bran for 10-15 minutes, take the ...
Embodiment 2
[0042] 1. Liquid culture efficient strain screening
[0043] It is preferred to culture a strain with a large number of bacterial balls, uniform size, and many burrs on the surface, the number of bacterial balls is 200-260 / ml, and the diameter of the bacterial balls is 1.0-1.5mm;
[0044] 2. shake flask culture
[0045] ① Shake flask medium formula: 1% glucose, 4% corn flour, 0.3% yeast powder, 2.0% bran, KH 2 PO 4 0.5%, MgSO 4 0.8%, PH value is natural.
[0046] ② Shake flask culture medium preparation: wash the shake flask first, then empty it, and the sterilization method is the same as the conventional one. Weigh the raw materials according to the formula of the shake flask medium, boil the potato slices for 15 minutes, filter and take the filtrate, boil the wheat bran for 15 minutes, take the filtrate after filtering, heat and dissolve the rest of the raw materials and bottle them together with the filtrate, the filling coefficient of the shake bottle 25%, sterilized...
Embodiment 3
[0059] 1. Liquid culture efficient strain screening
[0060] The preferred liquid culture strains have a large number of bacterial balls, uniform size, and many surface burrs, the number of bacterial balls is 200-260 / ml, and the diameter of bacterial balls is 1.0-1.5mm.
[0061] 2. shake flask culture
[0062] ① Shake flask medium formula: 3% glucose, 2% corn flour, 0.2% yeast powder, 1.0% bran, KH 2 PO 4 1.5%, MgSO 4 1.0%, PH value is natural.
[0063] ② Shake flask culture medium preparation: wash the shake flask first, then empty it, and the sterilization method is the same as the conventional one. Weigh the raw materials according to the formula of the shake flask medium, boil the potato slices for 10 minutes, filter and take the filtrate, boil the wheat bran for 10 minutes, filter and take the filtrate, heat and dissolve the rest of the raw materials and bottle them together with the filtrate, the filling factor of the shake bottle is 20 %, sterilized at a temperat...
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