Method for preserving edible fungus strains by utilizing Mongolian oak fruits
An edible fungus and fruit technology, applied in the fields of botanical equipment and methods, application, horticulture, etc., can solve the problems of short preservation time, slow growth of mycelium of bacteria, low fruiting amount, etc. The effect of a long time and a large amount of preservation
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Embodiment 1
[0022] Embodiment 1: Quercus mongolica fruit preservation medium preserves black fungus strains
[0023] 1. The strain activation medium used is:
[0024] PDA medium: peeled potato 200g, glucose 20g, agar 16g, water 1L.
[0025] PD medium: 200g of peeled potatoes, 20g of glucose, 1L of water.
[0026] 2. The tested strains are black fungus strains preserved in the Forest Protection Laboratory of Northeast Forestry University
[0027] 3. The specific steps are as follows:
[0028] (1) Prepare the PDA medium according to the conventional method, divide the PDA medium into test tubes, and the liquid volume of each test tube is 1 / 5-1 / 4 of the length of the test tube, and then put it in an autoclave for sterilization at 121°C After 20 minutes, the test tube was cooled to about 50°C, placed in a dry and clean environment to form a PDA slant medium, and cooled for later use.
[0029] (2) Inoculate black fungus strains with the size of soybean grains on PDA slant medium, and cultu...
Embodiment 2
[0040] Embodiment two: Sawdust preservation medium preserves Auricularia auricula species
[0041] (1) The formula of the sawdust preservation medium is 89% of broad-leaved sawdust, 10% of wheat bran, 0.5% of gypsum, 0.5% of lime, and the amount of water is 60% of the mass of the dry material. The above components are added in proportion, fully stirred, Mix well and set aside.
[0042] (2) Put the mixed culture medium into the test tube, compact the material with a glass rod when filling, the filling height is 2 / 3 of the test tube, wipe the mouth of the tube after loading, and plug the test tube stopper.
[0043] (3) sterilization mode is the same as embodiment (10)
[0044] (4) After the sterilization is completed, take out the medium and put it in a dry and clean environment, cool it down to about 28°C, insert 3mL of black fungus liquid strain, and culture it at 25°C until the mycelium is covered with the medium.
[0045] (5) After the hyphae are overgrown, seal the mouth ...
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