Agarose gel plate storage method

The technology of agarose gel and preservation method is applied in the field of medicine and health, and can solve the problems of agarose-prone bacteria, troublesome, short storage time, etc.

Inactive Publication Date: 2015-05-06
SHANGHAI DIAN CLINICAL TESTING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although great attention has been paid to the disinfection steps when preparing the gel plates, because the agarose is prone to bacteria growth, the time for the prepared agarose gel plates to be placed is very short, and often bacteria will grow and be scrapped in about a week; still need Do it now, it is more troublesome

Method used

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  • Agarose gel plate storage method
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  • Agarose gel plate storage method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 After making the experimental diagram on the day ( image 3 )

[0051] Take a fresh serum sample, use the preservative agarose gel plate made on the same day for protein electrophoresis experiment, and analyze the results:

[0052] swim lane ALB alpha 1 globulin alpha 2 globulin beta globulin gamma globulin 1 64.5 3.6 8.7 12.3 10.8 2 64.8 3.8 9.2 11.7 10.4 3 65.2 4.0 8.7 12.1 10.0 4 65.9 3.9 8.9 11.5 9.8 mean(x) 65.1 3.83 8.9 11.9 10.3 Standard Deviation(s) 0.52 0.15 0.20 0.32 0.38 Coefficient of Variation (CV%) 0.81 3.87 2.31 2.66 3.75

Embodiment 2

[0053] [0053] Example 2 Experimental diagram of samples stored in a refrigerator at 4°C for 3 months ( Figure 4 )

[0054]Take a sample and seal the antiseptic agarose gel plate with moisture in the box, moist appearance, sufficient moisture in the agar plate, plump, and no bacterial growth. Take a fresh serum sample, use the sample to do protein electrophoresis experiment, and analyze the results (because there is tailing in lane 4, the result is excluded):

[0055] swim lane ALB alpha 1 globulin alpha 2 globulin beta globulin gamma globulin 1 54.7 4.9 10.2 14.3 16.0 2 54.2 4.3 9.6 14.6 17.3 3 53.4 4.8 10.3 13.2 18.2 5 52.2 4.3 10.2 15.1 18.2 6 54.7 4.0 10.5 12.6 18.2 7 55.6 4.7 9.9 13.6 16.2 mean(x) 54.1 4.5 10.1 13.9 17.4 Standard Deviation(s) 1.19 0.35 0.32 0.93 1.03 Coefficient of Variation (CV%) 2.20 7.83 3.15 6.72 5.94

Embodiment 3

[0056] Example 3 Experimental diagram of samples stored in a refrigerator at 4°C for 4 months ( Figure 5 )

[0057] Take a sample and seal the antiseptic agarose gel plate with moisture in the box, moist appearance, sufficient moisture in the agar plate, plump, and no bacterial growth. Take a fresh serum sample, use the sample to do protein electrophoresis experiment, and analyze the results:

[0058] swim lane ALB alpha 1 globulin alpha 2 globulin beta globulin gamma globulin 1 61.2 2.8 7.9 12.4 15.7 2 63.0 2.5 8.1 10.1 16.2 3 60.2 2.8 7.1 13.0 16.8 4 60.7 2.6 7.1 11.7 17.9 5 60.0 2.6 7.2 11.8 18.5 6 62.4 2.8 6.3 11.6 16.9 7 62.1 2.7 7.2 11.4 16.6 8 62.3 3.0 6.5 11.5 16.8 9 61.7 2.7 8.2 11.9 15.6

[0059] 10 61.0 2.7 8.4 11.1 16.7 mean(x) 16.46 2.72 7.4 11.65 16.77 Standard Deviation(s) 1.00 0.14 0.72 0.76 0.89 Coefficient of Variat...

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Abstract

The present invention provides an agarose gel plate storage method, wherein a sealed wrapping material is wrapped outside the agarose gel plate, and the method comprises film covering, single packaging, boxing and bagging. Compared with the method in the prior art, the method of the present invention has the following characteristics that: in order to ensure the long-term effectiveness of the kit, a purpose of the present invention is to provide a method for long-term storage of the agarose gel plate, the agarose gel prepared by the method has characteristics of no bacterial growth, strong antiseptic performance and easy storage, and the experiment results show that the storage time of the agarose gel plate stored by the agarose gel plate storage method in the 4 DEG C refrigerator is up to 6 months, such that the problem of the too short agarose gel plate storage time is solved, and the great market value is provided.

Description

technical field [0001] The invention relates to the technical field of medicine and health, in particular to a method for preserving an agarose gel plate. Background technique [0002] Electrophoresis refers to the charged test substance (protein, nucleotide, etc.) in an inert support medium (such as starch gel, cellulose acetate, agarose gel, polyacrylamide gel, etc.), under the action of an electric field , swimming towards the corresponding electrode direction according to their respective speeds, so that the components are separated into narrow zones, and the method of recording the electrophoretic zone map or calculating its content (%) with a suitable detection method. The pH of the electrophoresis buffer is between 6 and 9, and the ionic strength is 0.02 to 0.05 as the optimum. 1% agarose is commonly used as the electrophoresis support. Although the resolution of agarose gel is lower than that of polyacrylamide gel, it is easy to prepare and has a wide range of sepa...

Claims

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Application Information

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IPC IPC(8): G01N27/447
Inventor 于嘉屏陆桂琴李艳梅王月婷
Owner SHANGHAI DIAN CLINICAL TESTING CENT
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