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Method for liquid fermentation cultivation of Agaricus bisporus strain

A technology of liquid fermentation and culture method, which is applied in the fields of botany equipment and methods, horticulture, application, etc., can solve the problems of not being able to meet the cultivation needs of Agaricus bisporus, the large amount of cultivated species required, and the high production cost, and achieve good economic benefits and Social effect, easy inoculation, and low production cost

Active Publication Date: 2014-05-21
江苏众友兴和菌业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Agaricus bisporus (Agaricus bisporus) is a kind of widely cultivated edible fungus. In the existing technology, the production of Agaricus bisporus generally adopts solid strains, but the solid strains have obvious defects, mainly in the long growth period, Inconsistent bacterial age, slow germination speed, large amount of cultivated species, high production cost, etc., obviously cannot meet the needs of large-scale Agaricus bisporus cultivation

Method used

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  • Method for liquid fermentation cultivation of Agaricus bisporus strain
  • Method for liquid fermentation cultivation of Agaricus bisporus strain
  • Method for liquid fermentation cultivation of Agaricus bisporus strain

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Take about 0.5cm of activated slant parent species 2 Inoculate the small pieces into the liquid culture medium, shake and cultivate the first-level shake flask strains at 25°C, and then transfer the cultured first-level shake flask liquid strains to the second-level liquid strain shake flasks for each treatment , carried out successively at all levels of culture, unless otherwise specified, all levels of shaking flask culture conditions are: 250mL Erlenmeyer flask liquid volume 100mL, inoculum size 5% (V / V), shaker speed 150r / min, 25 ℃ shaking culture 5 ~7d, similarly, the other levels of liquid strains are also cultivated in the same way. The liquid medium formula of Agaricus bisporus liquid strain is: nitrogen source, carbon source, KH 2 PO 4 3g, MgSO 4 1.5g, VB 1 10mg, water 1000mL, pH natural, sterilized at 121°C for 30min. The formula of PDA universal medium used for the slant culture of Agaricus bisporus: 200g potato, 20g sucrose, 1000mL water, natural pH,...

Embodiment 2

[0056] When the test tube is transferred to the liquid culture medium, it needs to obtain rich nutrients from the culture medium in time to quickly adapt to the liquid instead of the previous solid slant medium environment. Therefore, it is necessary to provide sufficient nutrients in the first-level liquid culture medium , shorten the adaptation process of mycelium growth, and obtain liquid strains that meet the standards. When the first-level liquid seeds are used as seeds to continue to expand the cultivation, the change of its growth environment needs to be considered. At this time, the impact of different carbon sources on its growth is most obvious. According to a certain amount of inoculum, the liquid seeds cultivated to the logarithmic growth phase under the optimal culture conditions of Example 1 are inoculated into the secondary liquid culture fluid, and cultivated in the secondary liquid culture fluid with different carbon sources selected. The results are as follows...

Embodiment 3

[0067] Adopt 10L full-automatic air-lift stirring fermenter tank, liquid capacity is 6L, by 5% inoculum size, under 25 ℃ of culture conditions, in liquid culture optimum medium (wheat bran 30g, corn flour 10g, potassium dihydrogen phosphate 0.5g , magnesium sulfate 1g, VB110mg / L, water 1000mL pH value 6.5) carry out liquid fermentation culture, through sampling analysis and record fermenter system monitoring data, each growth index change result of Agaricus bisporus liquid mycelium in air-lift fermenter is : Under the conditions of the fermenter, Agaricus bisporus maintained a good growth rate, and the biomass of mycelium balls increased significantly. In the early stage of fermentation, the mycelium density increased rapidly and remained at a stable value. The diameter of mycelium balls also increased to a certain extent as the fermentation process progressed, but the growth of mycelium ball diameter was still within a reasonable range. When the fermentation reached the 5th d...

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Abstract

The invention discloses a method for liquid fermentation cultivation of Agaricus bisporus strain, which includes: firstly, inoculating activated slant culture 0.5cm2 into a 250mL culture bottle containing 60-120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, a test tube of strain can be multiplied by 200000 times by five levels of cultivation, the secondary liquid strain is evidently faster than traditional solid spawn in growth speed, and the speed is increased by 33%. In addition, the growth speeds of all levels of the liquid strain are nearly equal, the average fullness time is 27.5 days, and the method is absolutely applicable to practice of factory production.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for liquid fermentation and cultivation of Agaricus bisporus strains. Background technique [0002] Agaricus bisporus (Agaricus bisporus) is a kind of widely cultivated edible fungus. In the existing technology, the production of Agaricus bisporus generally adopts solid strains, but the solid strains have obvious defects, mainly in the long growth period, Inconsistent bacterial age, slow germination speed, large amount of cultivated species, high production cost, etc., obviously cannot meet the needs of large-scale Agaricus bisporus cultivation. Contents of the invention [0003] Purpose of the invention: Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a liquid fermentation culture method of Agaricus bisporus strains, so as to meet the demand for efficient cultivation of Agaricus bisporus. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 李冠喜温以斌李荣花秦裕营寇运书孟德龙华国栋马腾樊继伟梁长东王多明
Owner 江苏众友兴和菌业科技有限公司
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