139 results about "Fungi culture" patented technology

The invention discloses a method for culturing rare edible fungi including tricholoma lobayense heim and clitocybe maxima by using vine shoot dust. The method comprises the following steps of: A, preparation of a fermentation culture solution; B, preparation of liquid spawn; C, preparation of granular spawn; D, preparation of a culture medium: selecting 68-72 percent of vine shoot dust, 8-12 percent of cotton seed hull, 8-12 percent of broken dry broad-leaved tree fallen leaves, 8 percent of bran, 1 percent of sucrose and 1 percent of calcined lime in a weight ratio, stirring in a material-water ratio of 1:1.4, bagging, performing conventional sealing, and sterilizing for later use; E, inoculating culture; and F, harvesting. The vine shoot is treated and developed into a raw material for culturing the rare edible fungi including the tricholoma lobayense heim and the clitocybe maxima, a natural culture medium is provided for the fungi culture industry, the biotransformation utilization of the vine shoot is realized, and the culture costs of the tricholoma lobayense heim and the clitocybe maxima are reduced.

A method for producing superior edible fungi culture medium by using manioc waste and acid-sludge as raw materials, belonging to the technical field of comprehensive utilization of the manioc waste and the acid-sludge, and edible fungi cultivation. In the invention, the manioc waste and the acid-sludge, two common leftover bits and pieces in fermentation and food industries, are scientifically compounded to serve as edible fungi culture medium, and the invention can be used for the production of all edible fungi culture media. The invention, on the basis of reducing culture medium raw material cost by over 50%, can shorten the culture period by 10-20% and increase the yield by 20-30%. The method resolves the problems of current low utility ratio of the manioc waste and the acid-sludge, serious environment pollution and the like, converts waste into treasure, and has high social and economic benefits.

The invention relates to an edible fungi culture medium, in particular to a medium for growing pleurotus eryngii by using grape branches. The medium for growing the pleurotus eryngii by using the grape branches is characterized in that: each 100 weight parts of culture medium comprises 63.89 to 79.69 weight parts of grape branches, 15.27 to 26.66 weight parts of bran, 3 to 8.16 weight parts of bean pulp and 3 to 6.85 weight parts of corn meal; and each 100g of culture medium comprises 68.03g of grape branches, 20.41g of bran, 8.16g of bean pulp and 3.40g of corn meal. The grape branches are taken as a main material and properly added with other biological nitrogen sources to form the medium for producing wooddecay fungi; and the culture medium can degrade cellulose, hemicelluloses and lignin in the grape branches, and produce high-quality edible fungi.

The invention relates to a method for preparing activated carbon by catalyzing and activating microorganism white-rot fungi or enzymes, which belongs to the technical field of activated carbon preparation. According to the preparation method, tobacco solid waste is used as a raw material, and a product is obtained through simple processes of pretreating the raw material, preparing a tobacco activated base by microorganism white-rot fungi culture or enzyme catalysis and activation and preparing the activated carbon. The method has the characteristics of wide raw material source, low waste utilization cost, low energy consumption in the production process, no secondary pollution, benefit to environmental protection, good adsorptive property and wide application of the activated carbon production, and the like. The method can be widely applied to preparing the activated carbon by taking the tobacco solid waste as a raw material, and the activated carbon prepared by adopting the method provided by the invention can be widely applied to the fields of waste water treatment, removal of SO2 from smoke, adsorption of odors in food fresh keeping, and the like.

The invention relates to an edible fungi culture medium additive, preparation and an application thereof. The edible fungi culture medium additive is prepared by raw materials rich in organic selenium, wherein, selenium content is 2.0-10.0mcg / g, and the raw materials rich in organic selenium comprise a corncob containing 0.2-30.0mcg / g organic selenium and a maize straw containing 0.5-50mcg / g organic selenium. The invention further provides a method for preparing the edible fungi culture medium additive: pulverizing the raw materials rich in organic selenium to obtain powder. The edible fungi cultured by the edible fungi culture medium additive of the invention has the advantages of stable content of the organic selenium, improved yield of the edible fungi by over 10%, low residual quantity of inorganic selenium and better safety.

The invention aims at disclosing a composite ecological cultivation method for tea, grass and fungi in a southern mountain young tea garden. The method is favorable for improving the physical property of the soil in the tea garden and improving the soil fertility level, and belongs to the technical field of composite ecological tea garden building, edible fungi culture, cyclic agriculture technology and tea garden soil improvement. According to the method, bean pasture chamaecrista rotundifolia and stropharia rugoso-annulata are sequentially interplanted in the southern mountain young tea garden. The composite ecological cultivation method has the advantages that the gap land and the growth characteristics of tea trees, the stropharia rugoso-annulata and the bean pasture chamaecrista rotundifolia are sufficiently utilized, pasture and edible fungi are introduced into the tea garden ecological system, the original matter content of the soil in the tea garden can be improved, the consumption of chemical fertilizers is reduced, the ecological environment of the tea garden is improved, the tea yield is favorably improved, the tea quality is improved, and the income of tea farmers is increased.

The present invention discloses an edible fungi culture medium and a preparation method thereof. The preparation method comprises: (1) carrying out a pretreatment on giant knotweed rhizome drug residue, wherein the pretreatment comprises adding water to giant knotweed rhizome drug residue and carrying out enzymolysis; and (2) preparing an edible fungi culture medium, wherein the treated giant knotweed rhizome drug residue is added with quick lime, gypsum, sucrose, cottonseed hulls and wheat bran, and uniform stirring is performed. According to the edible fungi culture medium, a resveratrol mass fraction is more than or equal to 0.1%. a crude protein mass fraction is more than or equal to 6%, a nitrogen-free extract mass fraction is more than ore equal to 30%, and a crude fiber mass fraction is more than or equal to 30%. According to the present invention, a complex enzyme preparation is adopted to carry out fermentation and enzymolysis to improve components of the giant knotweed rhizome drug residue, such that the components are applicable for cultures of pleurotus eryngii, cap fungus, coprinus comatus and other edible fungi.

The invention discloses a method for cultivating edible fungi, which comprises the following steps: using mulberry branches as the raw material to prepare cone-shaped particle and drying the corn-shaped particle; putting the dried mulberry branch particle into nutrient solution to be immersed until the nutrient solution is totally absorbed, wherein 100 parts by weight of dried mulberry branch particle is taken as the reference, and the weight proportion of the nutrient solution is that 170 to 277 parts of water, 13 to 18 parts of corn meal, 0.3 to 0.5 part of sucrose and 0.1 to 0.3 part of calcined lime; uniformly mixing the immersed mulberry branch particle with a fungi culture medium determined according to the type of the edible fungi, filling the mixture into a fungi bag or a fungi bottle, sterilizing, cooling, inoculating and cultivating the fungi; after hypha overgrows the whole fungi bag or the fungi bottle for 7 days, taking out the mulberry branch particle and inserting the mulberry branch particle into a fungi stick; and carrying out conventional treatment according to the type of the cultivated edible fungi after inoculating to obtain the edible fungi. The method does not need to use the device to punch and has simple and convenient operation, low production cost and low mixed bacterium infection rate. The harvest time of the edible fungi of the method is longer 20 to 30 days than that in the prior art.

The invention discloses a mixed culture base material for pleurotus eryngii culture. The base material comprises the following components in parts by weight: 3 to 5 parts of nutritional inorganic fertilizer, 60 to 70 parts of weed tree sawdust, 20 to 90 parts of oat husk, 10 to 40 parts of wood bran, 10 to 40 parts of peanut shell, 5 to 15 parts of cotton seed husk, 26 to 30 parts of calcium carbonate, 35 to 40 parts of corn core, 10 to 20 parts of sugarcane dreg, 15 to 25 parts of wheat bran, 3 to 8 parts of corn flour, 20 to 30 parts of calcium sulfate, 5 to 10 parts of magnesium sulfate, 5 to 10 pats of lime, 0.25 to 4 parts of gypsum, 5 to 10 parts of urea with a nitrogen content of 46%, 5 to 10 parts of triacontanol, and 2 to 7 parts of soybean meal. The water content of the mixed culture material after pre-wetting is 65% + / - 1%, and the pH value is 7.5 to 9. The nutritional inorganic fertilizer is a boron-potassium mixed fertilizer, and the mass ratio of boron fertilizer to potassium fertilizer is 1:1.5. The provided mixed culture base material is rich in nutrients such as vitamin B1, vitamin B, crude fiber, proteins, mineral substances, trace elements, and the like, and is beneficial for the growth of edible fungi. The edible fungi cultured by the provided mixed culture base material have a fresh and sweet flavor and a crispy and smooth taste.

The invention discloses a biological organic fertilizer special for soybean and a preparation method thereof. The biological organic fertilizer is prepared from the following raw materials in parts by weight by fermenting through a microbial agent: 15-24 parts of animal wastes, 20-26 parts of straws, 7-12 parts of wheat bran, 6-10 parts of fungi culture fungi residues, 2-6 parts of saw dust, 1-3 parts of maize meal, 1-3 parts of bean pulp and 10-19 parts of tap water, wherein the weight ratio of the microbial agent is composed of EM bacteria, saccharomycetes and nitrogen-fixing bacteria at a ratio of 3:1:1, and the inoculation amount of the microbial agent accounts for 0.1% of the weight of the raw materials. The preparation method disclosed by the invention can not only reduce the expense of treating the animal wastes, but also achieve the effect of waste utilization, solves the problem of pollution of the animal wastes on the environment, also solves the problem that the straws, the wheat bran and the fungi culture fungi residues are difficult to treat, meets the requirements of ecological circular agriculture and is low in cost and easy to operate.

The invention relates to a method for culturing edible fungi by utilizing fermentation baskets, fermentation tunnels and composting fermentation thereof, belonging to the technical field of edible fungi culture and production. With stackable fermentation baskets as containers, secondary fermentation is carried out in the fermentation tunnels. During the entire fermentation process, compost can not be turned after entering the tunnels; the temperature and moisture can be controlled so that the temperature in every part of the tunnel and water content of the compost are basically even. The method reduces the total time of fermentation and features good fermentation quality of compost, complete sterilization and high culture yield. The edible fungi are not limited by physioclimate and can be produced all year. The method is suitable for culturing edible fungi such as button mushroom, spring mushroom, agaricus blazei and coprinus comatus, etc.

The invention relates to an intelligent bagging charging machine. The intelligent bagging charging machine comprises a frame, the frame is provided with a blanking mechanism, an uninterrupted bag supply mechanism, a bag mouth opening mechanism, a four-pawl bagging mechanism, a bagging elevating mechanism, a bag holding mechanism, a tying mechanism, and a control cabinet controlling all the previous mechanisms. The blanking mechanism comprises a blanking hopper and a discharging pipe arranged at the bottom of the blanking hopper, and the discharging pipe is internally provided with a material extrusion propeller blade; and the uninterrupted bag supply mechanism comprises multiple liftable bag boxes and a bag suction device arranged above the bag boxes, the bag suction device comprises a vacuum chuck which can move back and forth above the bag boxes and is elevated up and down, and the area of the vacuum chuck is equal to the area of a culture bag. The apparatus provided by the invention is simple in structure and convenient to maintain, and can rapidly and effectively carry out charging tying work on the edible fungi culture bag.

The invention provides a method for culturing edible fungi by utilizing yew waste residue, comprising the following steps that: the waste residue of the yew after extraction of taxol is dried, crushed and mixed with other raw materials to prepare edible fungi culture medium; the culture medium is sterilized and filled into culture container separately; edible fungi spawns are inoculated into the culture containers for culture; and mycelium and encarpium of the edible fungi are obtained. The method not only takes the yew waste residue as a raw material to change the waste into the valuable and save the cost, but also has the characteristics of high biological effectiveness, good quality and rich nutrient, etc., and has obvious economic benefit and market benefit.

The invention provides a culture soil of edible fungus, which is composed of fermenting organic material fertilizer and raw soil, the preparation ratio of the raw soil and the fermenting organic material fertilizer is 100:20-30. The fermenting organic material fertilizer is prepared by fermenting material straw, waste residue of Chinese herbs, gross coal and brown coal by aerobic actinomycetes bacterial liquid, and then adding 1-2.5% amino acid, wherein the preparation ratio of each materials according to the weight percentage is that, straw takes 20-30, waste residue of Chinese herbs takes 20-30, gross coal takes 20-30 and brown coal takes 20-30. The culture soil contains various amino acid, humic acid, organic matter and the like which are needed for the fungus growth of culture soil and has high fertilizer efficiency, can be absorbed by the fungus quickly, leads mushroom to come out quickly, homogeneously, evenly and strongly. The invention has the advantages of high output, perfect quality, is suitable for culturing various edible fungus, and the time of the fertilizer efficiency lasts long and the time of preserving water is long.

The invention provides an edible fungi culture matrix prepared with fermentation fungi residues. The matrix is prepared by the following method: weighing cotton seed hulls or mulberry sawdust, fermentation waste fungi residues and a calcium magnesium phosphate fertilizer according to the following mass proportion and mixing uniformly: 60-80 parts of mixture of mulberry sawdust and bran or cotton seed hulls, 14-35 parts of fermentation waste fungi residue, 1-3 parts of calcium magnesium phosphate fertilizer, 0.5-2 parts of sucrose and 1-3 parts of gypsum; adding water to the mixed material until the water content is 65-68% by mass; adjusting the pH value to 7.0+ / -0.2 with lime; preserving heat for 8-12 hours in a boiler at 100-110 DEG C; and naturally cooling to obtain the edible fungi culture matrix. The invention provides a method for recycling the fermentation waste fungi residues, which ferments the industrial waste residues through pollution-free and green treatment and turns waste into wealth, and not only reduces the cost of edible fungi culture, but also increases the yield of edible fungi and realizes economic benefits.

The invention relates to processing machinery for producing edible fungi culture materials, in particular to a double-use plant material cutting grinder. The grinder comprises a frame, a cutting-grinding chamber arranged on the frame, a feeding hopper, a material outlet, a power device and a variable-speed transmission mechanism, wherein a public blanking hopper is directly arranged below the cutting-grinding chamber, namely at the bottom of a filter screen; a centrifugal material-sucking fan device is arranged in the public blanking hopper; the centrifugal material-sucking fan device consists of a pair of air impellers arranged on two sides of the same centrifugal fan main shaft ; and an air outlet of the centrifugal material-sucking fan device is communicated with the material outlet. The grinder can cut and grind straw of various plant materials, weeds, branches, woodwork shavings and other soft / hard raw materials, and helps to directly and smoothly suck out raw material particles even with high water content, thereby achieving the effects of reducing energy consumption, simplifying process and improving production efficiency.

The present invention relates to a fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof, wherein two fungi cultures such as Purpureocillium lilacinum CGMCC No.9344 and Clonostachys rosea CGMCC No.1037 are cultured to prepare the fungi mixing bacterial agent. According to the present invention, the fungi mixing bacterial agent can be mixed with seedling breeding mediums, organic fertilizers and organic-inorganic compound fertilizers or be used separately so as to perform soil treatment or hole application before or after transplantation, or root-irrigation, and is used for preventing and controlling plant meloidogyne diseases.

The invention discloses a water-rinsing-dung type large-scale dung comprehensive treatment method for a diary cattle farm. The method comprises the following processes of: 1) feeding collected dung, cattle house rinsing water and the like into a dung collection furrow, performing grid interception, separation, conditioning and homogenization and solid-liquid separation, airing and using the solids as cattle house padding, and ensuring liquid to enter a temporary storage pool; 2) feeding a portion of liquid in the temporary storage pool into an anaerobic fermentation system for treating to be used as wastewater for performing backwash of the system, and feeding the other portion of liquid into a multilevel biological cleaning storage pond through a sewage storage pool and connecting the multilevel biological cleaning storage pond with a filling mixed pool for regulating water quality to reach the safe agricultural irrigation level; 3) delivering dry and clean dung into a dung heaping field and using the dry and clean dung as edible fungi culture mediums and earthworm culture mediums, or directly selling or selling to persons who work in the planting industry after the dry and clean dung is made into composted fertilizers or organic fertilizers by means of stack retting. The water-rinsing-dung type large-scale dung comprehensive treatment method has the advantages that a water using method is optimized, so that the steps of production and drainage, treatment and utilization of sewage are performed in a virtuous circle mode; the method is high in process universality, low in operation cost and long in service life, has high capability of buffering and purifying the sewage, and is capable of effectively improving environmental hygiene.

The invention discloses a method for culturing fungi and planting tall gastrodia tuber indoors, which comprises the following steps of: firstly, taking raw materials comprising 90 to 110 parts of saw dust, 50 to 70 parts of wheat bran, 15 to 25 parts of corn flour, 1 to 3 parts of brown sugar and 1 to 3 parts of gypsum powder, and uniformly mixing the raw materials to obtain a mixed filler; and cleaning a fungi culture chamber, and fumigating and sterilizing the fungi culture chamber by using sulfur; partitioning a fungi material into small logs of 15 centimeters, binding the small logs and filling the small logs into a strain bag, tamping gaps in the strain bag by using the prepared mixed filler, then binding the bag mouth, performing sterilization, cooling and inoculation, and finally putting the strain bag into the fungi culture chamber. The growth and development states of hypha can be observed at any time and any place by indoor fungi culture, the growth and the development of the hypha can be affected by regulating factors such as temperature, moisture, illumination and the like for the growth of the hypha in time, the polluted strain bags are cleaned in time, the strain bags which do not produce the hypha can be used for secondary inoculation, and the bag mouths of the strain bags which produce the hypha slowly can be loosened to increase oxygen and promote the growth of the hypha, thus, improving the success rate of the fungi hypha.

The invention relates to fermentation liquid for culturing pleurotus eryngii matrix, a pleurotus eryngii culture medium and a preparation method thereof, belonging to the technical field of edible fungi culture. The culture matrix is prepared from the following raw materials: cottonseed hull, sawdust, corncob, bran, bagasse, bean stalk, rice bran, calcium carbonate and quick lime; the raw materials are uniformly blended and stockpiled for fermentation together with the microbial fermentation liquid of bacillus subtilis and bacillus circulans; and the decomposition and transformation of organic matters such as lignin and cellulose can be accelerated by use of inoculated fermentation composting, and the nutrient substances such as carbohydrates and carbon nitride compounds required for the mycelial growth of pleurotus eryngii are directly supplied. The pleurotus eryngii culture medium is fully used, the fruiting time is shortened, the biological conversion rate is increased, and the investment cost is saved.

The invention relates to the field of edible fungi culture, and in particular relates to a preservation culture medium for a hypsizygus marmoreus stock culture and a method for preparing the preservation culture medium. Firstly, the preservation culture medium for the hypsizygus marmoreus stock culture is provided, and has a culture medium formula by weight percent as follows: 50-55% of wood flour, 30%-40% of bran, 0.5%-1% of lime powder and 5%-10% of agar. The method for preparing the preservation culture medium is that the wood flour and the bran are mixed according to the proportion to be ground into particles, and then, the lime powder is added to mix uniformly; and the agar is heated to melt and then poured into the mixture of the wood flour, the bran and the lime powder, and water is appropriately added to make the water content of the mixture to be 55-60%. The hypsizygus marmoreus stock culture medium which is prepared by the method is simple and convenient to prepare and low in cost, and has longer preservation time; and moreover, the fungi activity is more strongly kept, the inoculated fungi has quick spawn running, disease resistance and higher heterozygosity resistance, and higher fungi yield of a single bottle is achieved.

The invention relates to a method for preparing edible fungi soluble polysaccharide, in particular to a method for extracting the soluble polysaccharide from fermentation products by taking bean dregs as raw materials and inoculating edible fungi for solid fermentation, belonging to the technical filed of solid fermentation by utilizing industrial residue. The method comprises the following steps of: taking the bean dregs as the raw materials, inoculating edible fungi culture for solid fermentation, and extracting the soluble polysaccharide from the fermentation products through hot water extraction and ethanol precipitation. The bean dregs are used as the raw materials for culturing edible fungi mycelium in a solid mode, then the soluble polysaccharide is extracted from the fermentation products, edible fungi polysaccharide with high added value is obtained, the purity of the edible fungi polysaccharide is higher than the traditional edible fungi mycelium polysaccharide obtained by solution culture, processing equipment is simple, the investment is less, bacteria are not easy to infect, and the product energy consumption is low.

The invention discloses an edible fungi culture medium, comprising, by weight, 5-9 parts of a Chinese herb additive, 6-8 parts of wheat bran, 4-6 parts of soybean meal, 15-25 parts of organic residue, 7-9 parts of agricultural straws, 1-3 parts of a trace element additive, 12-16 parts of calcined mineral powder, 20-30 parts of water, 2-4 parts of a PH regulator, 5-9 parts of peat, and 6-8 parts of coarse grain powder. The edible fungi culture medium has rich and balanced nutrition, is loose and breathable and provides greatly increased survival rate and quality for edible fungi; in addition, the Chinese herb additive allows the cultivated edible fungi to be good in safety, free of toxicity, fresh and sweet in taste, and crispy and smooth in taste with certain health effect; the preparation method is simple, and the materials are easy to obtain; a cultivation method of edible fungi herein is simple to perform, has low production cost, allows manpower and materials to be less wasted, provides improved production benefit and product quality, and has high practical value and a promising application prospect.

The invention belongs to the technical field of edible fungi production machinery. Edible fungi culture medium crushing equipment comprises a machine body frame, wherein a moving cutter capable of rotating is arranged in the machine body frame; a transmission shaft of the moving cutter is supported on the machine body frame through a bearing; the equipment comprises a separation plate having screen holes; the machine body frame is divided into a crushing cavity and a feeding cavity by the separation plate; upper and lower cutter bases are arranged on the wall of the crushing cavity; a material channel is formed between the upper and lower cutter bases, and is communicated with a material inlet on the wall of the crushing cavity; the moving cutter is positioned in the crushing cavity; a free end of the transmission shaft of the moving cutter is fixedly connected with a crankshaft; a hammer body is arranged on a crank throw of the crankshaft, and is provided with a strip-shaped hole; the crankshaft is inserted in the strip-shaped hole of the hammer body; and the hole length of the strip-shaped hole is greater than the diameter of the crank throw of the crankshaft. Compared with a traditional crusher, the equipment is only required to be provided with the crankshaft and the hammer body to achieve the purpose of improving the material fineness; and compared with a newly added high-rotating-speed motor with high cost, the crankshaft and the hammer body are lower in cost.

The invention discloses a degradable edible fungi culturing bag with mushroom growing on the periphery, belonging to culturing technical field of edible fungi. The degradable edible fungi culturing bag adopts the components and the weight shares of the components as following: high density polyethylene 70 to 80 shares, linear low density polyethylene 10 to 20 shares, polylactic acid 3 to 10 shares, polyhydroxyalkanoate 0 to 5 shares, polybutylene succinate 0 to 5 shares, antioxidant phosphorous acid triester 0.1 to 0.2 share, calcium stearate 0.1 to 0.5 share, zinc stearate 0.1 to 0.2 share and aluminate ester resin acceptor 0.1 to 0.2 share. The mushroom can self break the film to grow on the periphery. The degradable edible fungi culturing bag with mushroom growing on the periphery has the advantages of improving the biotransformation rate and the benefit by above 30 percent and shortening the culturing and fruiting cycle by about 30 percent and also has the advantages of good degradability and low cost. The raw materials are easily obtained, so the degradable edible fungi culturing bag is suitable for popularizing in edible fungi culturing area.

A method for producing superior edible fungi culture medium by using manioc waste and acid-sludge as raw materials, belonging to the technical field of comprehensive utilization of the manioc waste and the acid-sludge, and edible fungi cultivation. In the invention, the manioc waste and the acid-sludge, two common leftover bits and pieces in fermentation and food industries, are scientifically compounded to serve as edible fungi culture medium, and the invention can be used for the production of all edible fungi culture media. The invention, on the basis of reducing culture medium raw material cost by over 50%, can shorten the culture period by 10-20% and increase the yield by 20-30%. The method resolves the problems of current low utility ratio of the manioc waste and the acid-sludge, serious environment pollution and the like, converts waste into treasure, and has high social and economic benefits.

The invention discloses a method and a device for sealing an edible fungi culture bag. A plastic rod, a sleeve ring and a sleeve cover and a method for sealing a bag opening are adopted for sealing, and the invention is characterized in that: the prepared culture material is mechanically conveyed to a hopper and is automatically packaged into a beveled polypropylene bag with the specification of 17cm*35cm*0.005cm by an automatic bag packaging machine; an operator takes a culture bag filled with materials down, rapidly puts the bag into a transfer container, timely inserts the plastic rod into a reserved hole in the culture bag and transmits the bag to the next process through a transmission belt; and the next operator sleeves a plastic sleeve ring on the bag opening, compacts the material surface, extracts the plastic rod, covers the sleeve cover and puts the bag into a sterilization trolley for high-pressure sterilization. The method and the device for sealing the edible fungi culture bag have the advantages of shortening the inoculation time, reducing the pollution, contributing to mycelia germination, greatly shortening the culture time, improving the utilization rate of mushroom houses, saving energy, reducing the cost and contributing to large-scale factory production.

The present invention relates to modified nucleotide sequences encoding serum albumin, wherein the mRNA sequence has been codon optimized for expression in a filamentous host cell. Furthermore the present invention relates to serum albumin produced in filamentous fungi or loaded by contacting serum albumin with an extract of a filamentous fungi culture. In an-other aspect the present invention relates to the use of said serum albumin in serum free cell culture media and also to a method of drying and agglomerating serum albumin thereby im-proving wettability and dispersability.

The present invention relates to modified nucleotide sequences encoding serum albumin, wherein the mRNA sequence has been codon optimized for expression in a filamentous host cell. Furthermore the present invention relates to serum albumin produced in filamentous fungi or loaded by contacting serum albumin with an extract of a filamentous fungi culture. In an-other aspect the present invention relates to the use of said serum albumin in serum free cell culture media and also to a method of drying and agglomerating serum albumin thereby im-proving wettability and dispersability.