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Biocontrol fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof

A technology for biocontrol of fungi and knot nematodes, which is applied in the fields of agricultural, forestry and horticultural crop protection, can solve the problems of declining control effect of tomato root-knot nematode disease and reducing the population density of banana perforating nematodes, etc.

Active Publication Date: 2016-01-27
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mendoza and Sikora (2009) showed that the use of avirulent Fusarium that induces systemic resistance in plants and Bacillus firmus that has a strong lethal effect on root-knot nematode J2 can effectively reduce the population density of banana perforator nematodes; Khan et al. (2001) will The control effect of P. lilacinum and Trichoderma on tomato root-knot nematode after mixed use is higher than that of a single biocontrol fungus; Frans et al. (1992) mixed Verticillumchlamydosporium and Pasteuriapenetrans to treat the soil, Anastasiadis et al. (2008 ) mixed use of P. lilacinus and Bacillus firmus to treat the soil before transplanting and re-applying when transplanting can improve the control effect on tomato root-knot nematode; but the mixed use of endophytic bacteria and actinomycetes (Peng Shuang et al., 2012), the mixed use of F.oxysporum and Rhizobiumetli has a decreased control effect on tomato root-knot nematode (Martinuz, 2012)

Method used

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  • Biocontrol fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof
  • Biocontrol fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof
  • Biocontrol fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof

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Experimental program
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Effect test

specific Embodiment 1

[0010] Specific example 1: the prevention and control effect of P. lilacinus HS-12 and Clonosporium pinkus HLD-1 mixed powder on root-knot nematode

[0011] The experiment was carried out at the Langfang Base of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences. Including four treatments of HS-12, HLD-1, HS-12+HLD-1, and blank control, 30 seedlings per treatment, 3 replicates, randomly arranged in a plot with an area of ​​6m x 0.7m. Before the cucumber was transplanted, the nematode soil was collected by the five-point sampling method, and the nematode density in the plot was determined to be 300 eggs / 100g dry soil by the sucrose centrifugal flotation method. Mix the single bacterial agent and the mixed bacterial agent with a small amount of sand and substrate (sand: substrate = 1:1), so that the final concentration of biocontrol bacteria reaches 10 6 cfu / g, applied in holes when transplanting, 30-50g per hole. For each treatment, 30 cucumber seedli...

specific Embodiment 2

[0017] Specific embodiment 2. the prevention and control effect of P. lilacensis HS-12 and Clonosporium pinkhelix HLD-1 mixed liquid bacterial agent on root-knot nematode disease

[0018] The experiment was carried out in Experimental Field 1 and Experimental Field 2 of the Langfang Base of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences. Each test field is divided into 12 plots (length 6m×width 0.7m), which are divided into four treatments of HS-12, HLD-1, HS-12+HLD-1 and blank control, and three plots are repeated for each treatment, arranged randomly . 30 seedlings were sown per treatment. 100mL5×10 cucumbers were used 15 days before transplanting, when transplanting and 10 days after transplanting. 6 Spores / mL of HS-12, HLD-1 and mixed liquid fungal agents for root irrigation (Table 3).

[0019] Table 3 HS-12 and HLD-1 liquid bacterial agents and their combined field treatment methods

[0020]

[0021] 15 days before transplanting, the so...

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Abstract

The present invention relates to a fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof, wherein two fungi cultures such as Purpureocillium lilacinum CGMCC No.9344 and Clonostachys rosea CGMCC No.1037 are cultured to prepare the fungi mixing bacterial agent. According to the present invention, the fungi mixing bacterial agent can be mixed with seedling breeding mediums, organic fertilizers and organic-inorganic compound fertilizers or be used separately so as to perform soil treatment or hole application before or after transplantation, or root-irrigation, and is used for preventing and controlling plant meloidogyne diseases.

Description

technical field [0001] The invention belongs to the technical field of protection of agriculture, forestry and horticultural crops, in particular to a biocontrol fungus mixed bacterial agent for preventing and controlling plant root-knot nematode and its application. technical background [0002] Root-knot nematode (Meloidogynes pp.) is an important class of plant parasitic nematodes, with a wide range of hosts, more than 3,000 species of plants, and often causes serious economic losses, which can cause nearly 100 billion US dollars in losses every year worldwide. In recent years, with the continuous expansion of greenhouse vegetable planting area, its occurrence has also increased year by year, and the yield loss can reach 30% to 50%. [0003] At present, the control of root-knot nematodes is mainly based on chemical nematicides, such as chloropicrin, methyl bromide, metabam, and aldicarb. Although chemical nematicides have quick effects, they are expensive, seriously poll...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P5/00C05G3/02C05G3/60
Inventor 李世东郭荣君张家家孙漫红缪作清
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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