The invention discloses a domestic fungus culture medium. The culture medium comprises, by weight, 15-20 parts of bean residues, 20-30 parts of rick husks, 15-25 parts of cassava stalk powder, 15-25 parts of spinach leaf powder, 10-15 parts of potatoes, 10-20 parts of corn flour, 5-10 parts of beef extract, 10-20 parts of guava leaves, 1-5 parts of vitamins, 2-8 parts of ammonium sulfate, 1-6 parts of gypsum, 1-3 parts of urea, 2-10 parts of Chinese yam powder, 2-10 parts of Spica Prunellae, 1-5 parts of iron citrate, 2-10 parts of silkworm chrysalis powder, 1-5 parts of licorice root and 1-6 parts of fermented chicken manure. The culture medium has the advantages of fast spawn running speed, short culture period, high output, low cost, simple composition, improvement of the output and the nutrition of edible fungi, enhancement of the disease resistance and the competitor infection resistance of the edible fungi, effective killing of competitors and viruses, guaranteeing of the healthy growth of the edible fungi, no pollution, no pesticide residual and low content of heavy metals, and is suitable for culturing Pleurotus eryngii, needle mushroom, Pleurotus citrinopileatus, Pleurotus ostreatus, Pleurotus nebrodensis, shiitake, edible tree fungus and various other edible fungi.