Medium for growing pleurotus eryngii by using grape branches
A technology for eryngium eryngii and branches, which is applied in the field of substrates for planting eryngium eryngii, can solve the problems of low application rate, slow rate of return of branches to the field and easy to cause diseases and insect pests, environmental pollution, etc., so as to save resources and simplify the degradation path of reuse. , the effect of avoiding production process and environmental pollution
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[0023] 1. Cultivation of PDA first-class material - mother species (first-class species)
[0024] 1. Prepare PDA enriched medium: potato 200g / L, glucose 10g / L, sucrose 10g / L, peptone 1g / L, agar 12g / L, KH 2 PO 4 1g / L, MgSO 4 0.5g / L, pH7.0
[0025] 2. Sterilize for 30 minutes, make a slope
[0026] 3. Inoculate and culture at 23°C for 7-10 days in the dark
[0027] two. Propagation of mother species - original species (secondary species)
[0028] 1. Preparation of wheat grain medium: boil the wheat for half an hour, add 10g / kg of glucose, mix well, let it cool down and pack it into bags.
[0029] 2. Sterilize for 30 minutes, then insert the first-grade species after cooling
[0030] 3. Cultivate at constant temperature and dark at 23°C for about 20 days
[0031] 3. Propagation of original species - cultivated species
[0032] 1. Prepare cultivation material: Mix 68.03 grams of grape branch powder, 20.41 grams of bran, 3.40 grams of corn flour, and 8.16 grams of soybean ...
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