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Method for preparing edible fungi soluble polysaccharide through solid fermentation by taking bean dregs as raw materials

A technology of solid fermentation and edible fungus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low added value and low extraction rate of products, and achieve the goal of not being easily infected with miscellaneous bacteria and energy consumption of products Low, the effect of preventing the agglomeration of culture material

Inactive Publication Date: 2010-09-15
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology still has the problems of low extraction rate (20-40%) and low added value of the product (soybean soluble polysaccharide)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Mix bean dregs and olive dregs at a weight ratio of 1:1, add water to adjust the moisture content of the compost to 70% (w / w); put the compost into an edible fungus culture bottle, stuff the mouth of the bottle with a cotton plug, and extinguish at 121°C. bacteria for 30 minutes; after cooling, insert the rare edible fungus Pleurotus chinensis strain into the compost, cultivate for 30 days at 25°C and 70% humidity, take out the mycelium compost from the culture bottle, add 10 times the weight of water, 80 °C for 1 hour in a water bath, then centrifuged at 4000 rpm for 2 minutes, and collected the supernatant. Add 4 times the volume of 95% ethanol to the supernatant, centrifuge at 4000rpm for 2 minutes, collect the precipitated polysaccharide, and dry the precipitated polysaccharide in a low-temperature vacuum to obtain a powdered water-soluble edible fungus polysaccharide.

[0017] According to the amount of polysaccharide extracted corresponding to the mycelium biomass...

Embodiment 2

[0020] Mix bean dregs and olive dregs at a weight ratio of 1:2, add water to adjust the moisture content of the culture material to 75% (w / w); put the culture material into an edible fungus culture bag, put a cotton plug on the bag mouth, and extinguish at 121°C. bacteria for 30 minutes; after cooling, insert the rare edible fungus Pleurotus chinensis strain into the compost, cultivate for 30 days at 25°C and humidity 70%, take out the mycelium compost from the culture bag, add 20 times the weight of water, 60 °C for 3 hours in a water bath, then centrifuged at 4000 rpm for 10 minutes, and collected the supernatant. Add 4 times the volume of 95% ethanol to the supernatant, centrifuge at 4000rpm for 10 minutes, collect the precipitated polysaccharide, and dry the precipitated polysaccharide in a low-temperature vacuum to obtain a powdered water-soluble edible fungus polysaccharide.

[0021] After testing, in the solid fermentation product, the weight of the mycelia of Pleurotus...

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Abstract

The invention relates to a method for preparing edible fungi soluble polysaccharide, in particular to a method for extracting the soluble polysaccharide from fermentation products by taking bean dregs as raw materials and inoculating edible fungi for solid fermentation, belonging to the technical filed of solid fermentation by utilizing industrial residue. The method comprises the following steps of: taking the bean dregs as the raw materials, inoculating edible fungi culture for solid fermentation, and extracting the soluble polysaccharide from the fermentation products through hot water extraction and ethanol precipitation. The bean dregs are used as the raw materials for culturing edible fungi mycelium in a solid mode, then the soluble polysaccharide is extracted from the fermentation products, edible fungi polysaccharide with high added value is obtained, the purity of the edible fungi polysaccharide is higher than the traditional edible fungi mycelium polysaccharide obtained by solution culture, processing equipment is simple, the investment is less, bacteria are not easy to infect, and the product energy consumption is low.

Description

technical field [0001] The invention relates to a method for preparing edible fungus soluble polysaccharides, in particular to a method of using bean dregs as a culture material, inoculating edible fungi for solid fermentation, thereby extracting soluble polysaccharides in fermentation products, belonging to the technical field of solid fermentation using industrial waste residues . Background technique [0002] my country is a big producer and consumer of soybeans, processing about 25 million tons of soybeans annually. Among them, about 14 million tons are used to make oil, while 10 million tons are used to make soybean products or other foods. Every time 1kg of soybeans are processed to produce soy products, about 0.3-0.4kg of bean dregs will be produced, which is quite a large amount. Dried bean dregs contain 15-25% protein and 40-60% dietary fiber. Due to the tight combination of soybean protein and cellulose, bean dregs are difficult to be decomposed by enzymes in th...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/645
Inventor 郑恒光陈君琛沈恒胜汤葆莎
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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