Chinese saprophytic boletus liquid strain culture medium and culture method

Abstract

The invention belongs to the field of microorganisms, and discloses a Chinese saprophytic boletus liquid strain culture medium and a culture method. The culture medium is prepared from 150 to 200 g of potatoes, 10 to 25 g of bran, 10 to 15 g of glucose, 2 to 3 g of yeast powder, 1 to 2 g of CaCO3, 2 to 3 g of KH2PO4, 1 to 2 g of MgSO4, 1 to 2 tablets of vitamin B1 and 1000 mL of water. The culture medium provided by the invention is suitable for rapid growth of the Chinese saprophytic boletus, the rotating speed of a shaking table is controlled in different periods according to the growth characteristics of mycelia in the culture process, the recovery, germination and growth of the mycelia are promoted, the method is simple to operate and low in cost, mycelium pellets are fine and uniform, after mushroom sticks are inoculated, the mycelium pellets germinate in a multi-point mode, and therefore, the contact area between the mycelia and the culture material is effectively increased; compared with a solid strain, the mycelia are high in growth speed, low in pollution rate and consistent in strain age, and the strain culture period is effectively shortened.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a liquid culture medium and a culture method of boletus saprophyticus sinensis. Background technique [0002] Yunnan Institute of Tropical Crops Science and Hainan Medical College conducted molecular identification and biological characteristics research on wild Boletus sinensis collected from Xishuangbanna Prefecture, Yunnan Province and Haikou City, Hainan Province. In April 2021, Buchwaldoboletus xylophilus Named "Boletus saprophyticus", the Boletus saprophyticus strain BU001 was preserved in the General Microbiology Center of China Committee for Microbial Culture Collection on May 17, 2021, and the preservation number is: CGMCC No.: 21959. Address of Preservation Unit: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. [0003] Chinese saprophytic boletus (Buchwaldoboletus xylophilus) belongs to Fungi Kingdo...

AI Technical Summary

Problems solved by technology

[0004] The Chinese saprophytic boletus cultivars are inoculated with solid strains, the germination time of the strains is long, and the mycelium grows slowly, resulting in a long preparation period for the Chinese saprophytic boletus cultivars, a high pollution rate, and inconsistent growth of bacterial sticks, which restricts the growth of the Chinese saprophytic boletus. Industrialization and large-scale cultivation of saprophytic boletus