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Method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress

A technology of suspension cells and Luo Han Guo, applied in plant cells, fermentation, etc., to achieve the effects of increased biomass, increased yield, and higher specific growth rate of cells

Active Publication Date: 2018-07-06
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, a lot of research has been done on the physiological, biochemical, and molecular biological response mechanisms of trees to salt, but there is no report on the effect of salt stress and potassium-sodium ratio on the suspension cells of Luo Han Guo

Method used

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  • Method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress
  • Method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress
  • Method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Step 1: Take the mature seed embryo of Luo Han Guo, and put it in B5 containing 25 g / L of sucrose, 0.05 mg / L of 6-benzylaminoadenine, 2 mg / L of naphthalene acetic acid, 50 mg / L of inositol, 4 g / L of agar, and a pH of 5.9. Subculture on solid medium, select the fresh embryogenic callus that has been subcultured continuously for 3 times and has vigorous growth, loose texture, and stable and uniform state; Step 2: Inoculate the fresh embryogenic callus obtained in Step 1 with an inoculation amount of 25 g / L was transferred to the B5 liquid medium containing sucrose 20g / L, 6-benzylaminoadenine 0.05mg / L, naphthalene acetic acid 3mg / L, inositol 50mg / L, pH 5.9, and at a speed of 80r / min, cultured at 24°C in a dark shaker to obtain the first-generation Luo Han Guo suspension cell system.

[0034] Step 3: Cultivate mature Luo Han Guo suspension cells with the first-generation Luo Han Guo suspension cell system obtained in Step 2; the ratio of potassium to sodium in the origina...

Embodiment 2

[0038] Step 1: Take the seed embryo of Luo Han Guo and place it on a B5 solid containing 35 g / L of sucrose, 0.15 mg / L of 6-benzylaminoadenine, 6 mg / L of naphthalene acetic acid, 150 mg / L of inositol, 6 g / L of agar, and a pH of 6.0 Subculture on the culture medium, select the embryogenic callus that has been subcultured for 5 times;

[0039]Step 2: Transfer the embryogenic callus tissue obtained in Step 1 to the inoculum containing 40 g / L of sucrose, 0.15 mg / L of 6-benzylaminoadenine, 5 mg / L of naphthalene acetic acid, and 150 mg / L of inositol at an inoculation amount of 75 g / L. , pH 6.0 B5 liquid medium, and cultivated in a dark shaker with a rotation speed of 150r / min and a culture temperature of 26°C to obtain the first-generation Luo Han Guo suspension cell system.

[0040] Step 3: Cultivate mature Luo Han Guo suspension cells with the first-generation Luo Han Guo suspension cell system obtained in Step 2; the ratio of potassium to sodium in the original medium is 25:1, and...

Embodiment 3

[0044] Step 1: Get the mature seed embryo of Luo Han Guo, and add 30 g / L of sucrose, 0.1 mg / L of 6-benzylaminoadenine, 4.0 mg / L of naphthalene acetic acid, 100 mg / L of inositol, 5.0 g / L of agar, and a pH range of Subculture on the B5 solid medium of 5.9, select fresh embryogenic callus that has been subcultured for 4 times and has vigorous growth, loose texture, stable and uniform state;

[0045] Step 2: Transfer the fresh embryogenic callus tissue obtained in step 1 to an inoculum containing 30 g / L of sucrose, 0.1 mg / L of 6-benzylaminoadenine, 4.0 mg / L of naphthalene acetic acid, and 100 mg of inositol with an inoculation amount of 50 g / L. / L, pH range of 5.9 in the B5 liquid medium, and cultured in a shaker with a rotation speed of 110r / min, a culture temperature of 25°C, and a dark shaker to obtain the first-generation Luo Han Guo suspension cell system;

[0046] Step 3: Cultivate mature Luo Han Guo suspension cells with the first-generation Luo Han Guo suspension cell syst...

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Abstract

The invention relates to a method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress. The method is characterized by comprising the steps: controlling a potassium ion concentration to be 100-2000 mg / kg in a process of culturing mature momordica grosvenori suspension cells by a primary momordica grosvenori suspension cell system, wherein a potassium-sodium ion concentration ratio is (5-20):1. According to the method disclosed by the invention, the growth amount and the specific growth rate of momordica grosvenori and the yield and synthesis rate of the mogroside V are improved, and momordica grosvenori suspension cell growth and large-scale market production of the mogroside V are facilitated; meanwhile, the production and culture cycle of cells is effectively shortened, the biomass of the momordica grosvenori suspension cells and the production process of the mogroside V are regulated in a manual manner, and quality control and management of the product can be facilitated.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for promoting the growth of monk fruit suspension cells and increasing the content of sweet glycoside V through salt stress. Background technique [0002] Mogroside V, also known as mogroside, is the main sweet component of Luo Han Guo in Cucurbitaceae, and its sweetness is 350 times that of sucrose. Currently, seven monomer components have been identified, among which mogroside V and Sai The sweetness of phylloside Ⅰ is the strongest. Mogroside Ⅴ has medicinal effects such as clearing away heat and moistening the lungs, soothing the throat, relaxing the bowels, etc. At the same time, Mogroside Ⅴ also has the characteristics of novel taste and pure taste, which makes it deeply loved by people. It is the most suitable sweetener and health care product for special groups such as patients with diabetes, high blood pressure, and diabetes, and has a wide range of application val...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12P33/00
CPCC12N5/04C12P33/00
Inventor 郭美锦宋云飞陈雨霞王泽建李杰杨文国庄英萍储炬肖慈英
Owner EAST CHINA UNIV OF SCI & TECH
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